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Hydrogel Containing Anti-CD44-Labeled Microparticles, Guide Bone Tissue Formation in Osteochondral Defects in Rabbits

E. Filová, Z. Tonar, V. Lukášová, M. Buzgo, A. Litvinec, M. Rampichová, J. Beznoska, M. Plencner, A. Staffa, J. Daňková, M. Soural, J. Chvojka, A. Malečková, M. Králíčková, E. Amler,

. 2020 ; 10 (8) : . [pub] 20200731

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc20021905

Grantová podpora
16-29680A and 17-32285A the Ministry of Health of the Czech Republic
18-09306S and CTATL03000207 the Czech Science Foundation
project no. LO1304 the National Program of sustainability
projects No. LO1605, LO1309, LO1508 the Ministry of Education, Youth and Sports within National Sustainability Programme I
NV17-32285A MZ0 CEP - Centrální evidence projektů

Hydrogels are suitable for osteochondral defect regeneration as they mimic the viscoelastic environment of cartilage. However, their biomechanical properties are not sufficient to withstand high mechanical forces. Therefore, we have prepared electrospun poly-ε-caprolactone-chitosan (PCL-chit) and poly(ethylene oxide)-chitosan (PEO-chit) nanofibers, and FTIR analysis confirmed successful blending of chitosan with other polymers. The biocompatibility of PCL-chit and PEO-chit scaffolds was tested; fibrochondrocytes and chondrocytes seeded on PCL-chit showed superior metabolic activity. The PCL-chit nanofibers were cryogenically grinded into microparticles (mean size of about 500 µm) and further modified by polyethylene glycol-biotin in order to bind the anti-CD44 antibody, a glycoprotein interacting with hyaluronic acid (PCL-chit-PEGb-antiCD44). The PCL-chit or PCL-chit-PEGb-antiCD44 microparticles were mixed with a composite gel (collagen/fibrin/platelet rich plasma) to improve its biomechanical properties. The storage modulus was higher in the composite gel with microparticles compared to fibrin. The Eloss of the composite gel and fibrin was higher than that of the composite gel with microparticles. The composite gel either with or without microparticles was further tested in vivo in a model of osteochondral defects in rabbits. PCL-chit-PEGb-antiCD44 significantly enhanced osteogenic regeneration, mainly by desmogenous ossification, but decreased chondrogenic differentiation in the defects. PCL-chit-PEGb showed a more homogeneous distribution of hyaline cartilage and enhanced hyaline cartilage differentiation.

Citace poskytuje Crossref.org

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$a Hydrogels are suitable for osteochondral defect regeneration as they mimic the viscoelastic environment of cartilage. However, their biomechanical properties are not sufficient to withstand high mechanical forces. Therefore, we have prepared electrospun poly-ε-caprolactone-chitosan (PCL-chit) and poly(ethylene oxide)-chitosan (PEO-chit) nanofibers, and FTIR analysis confirmed successful blending of chitosan with other polymers. The biocompatibility of PCL-chit and PEO-chit scaffolds was tested; fibrochondrocytes and chondrocytes seeded on PCL-chit showed superior metabolic activity. The PCL-chit nanofibers were cryogenically grinded into microparticles (mean size of about 500 µm) and further modified by polyethylene glycol-biotin in order to bind the anti-CD44 antibody, a glycoprotein interacting with hyaluronic acid (PCL-chit-PEGb-antiCD44). The PCL-chit or PCL-chit-PEGb-antiCD44 microparticles were mixed with a composite gel (collagen/fibrin/platelet rich plasma) to improve its biomechanical properties. The storage modulus was higher in the composite gel with microparticles compared to fibrin. The Eloss of the composite gel and fibrin was higher than that of the composite gel with microparticles. The composite gel either with or without microparticles was further tested in vivo in a model of osteochondral defects in rabbits. PCL-chit-PEGb-antiCD44 significantly enhanced osteogenic regeneration, mainly by desmogenous ossification, but decreased chondrogenic differentiation in the defects. PCL-chit-PEGb showed a more homogeneous distribution of hyaline cartilage and enhanced hyaline cartilage differentiation.
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$a Litvinec, Andrej $u Department of Tissue Engineering, Institute of Experimental Medicine of the Czech Academy of Science, Videnska 1083, 142 20 Prague 4, Czech Republic.
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$a Beznoska, Jiří $u Hospital of Rudolfa and Stefanie, a. s., Máchova 400, 256 30 Benešov, Czech Republic.
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$a Daňková, Jana $u Department of Tissue Engineering, Institute of Experimental Medicine of the Czech Academy of Science, Videnska 1083, 142 20 Prague 4, Czech Republic.
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$a Soural, Miroslav $u Department of Organic Chemistry, Faculty of Science, Palacky University, 17. listopadu 12, 771 46 Olomouc, Czech Republic.
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$a Chvojka, Jiří $u Faculty of Textile Engineering, Technical University of Liberec, Studentská 2, 461 17 Liberec, Czech Republic.
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$a Amler, Evžen $u Department of Tissue Engineering, Institute of Experimental Medicine of the Czech Academy of Science, Videnska 1083, 142 20 Prague 4, Czech Republic. Institute of Biophysics, 2nd Faculty of Medicine, Charles University, V Uvalu 84, 150 06 Prague 5, Czech Republic. Student Science s.r.o., Národních Hrdinů 279, Dolní Počernice, 190 12 Prague, Czech Republic.
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