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Acrosin is essential for sperm penetration through the zona pellucida in hamsters
M. Hirose, A. Honda, H. Fulka, M. Tamura-Nakano, S. Matoba, T. Tomishima, K. Mochida, A. Hasegawa, K. Nagashima, K. Inoue, M. Ohtsuka, T. Baba, R. Yanagimachi, A. Ogura,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Free Medical Journals
from 1915
Freely Accessible Science Journals
from 1915 to 6 months ago
PubMed Central
from 1915 to 6 months ago
Europe PubMed Central
from 1915 to 6 months ago
Open Access Digital Library
from 1915-01-15
Open Access Digital Library
from 1915-01-01
- MeSH
- Acrosin genetics metabolism MeSH
- Acrosome metabolism MeSH
- Fertilization in Vitro MeSH
- Gene Knockout Techniques MeSH
- Sperm-Ovum Interactions * MeSH
- Cricetinae genetics metabolism MeSH
- Spermatozoa enzymology physiology MeSH
- Zona Pellucida metabolism MeSH
- Animals MeSH
- Check Tag
- Cricetinae genetics metabolism MeSH
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
During natural fertilization, mammalian spermatozoa must pass through the zona pellucida before reaching the plasma membrane of the oocyte. It is assumed that this step involves partial lysis of the zona by sperm acrosomal enzymes, but there has been no unequivocal evidence to support this view. Here we present evidence that acrosin, an acrosomal serine protease, plays an essential role in sperm penetration of the zona. We generated acrosin-knockout (KO) hamsters, using an in vivo transfection CRISPR/Cas9 system. Homozygous mutant males were completely sterile. Acrosin-KO spermatozoa ascended the female genital tract and reached ovulated oocytes in the oviduct ampulla, but never fertilized them. In vitro fertilization (IVF) experiments revealed that mutant spermatozoa attached to the zona, but failed to penetrate it. When the zona pellucida was removed before IVF, all oocytes were fertilized. This indicates that in hamsters, acrosin plays an indispensable role in allowing fertilizing spermatozoa to penetrate the zona. This study also suggests that the KO hamster system would be a useful model for identifying new gene functions or analyzing human and animal disorders because of its technical facility and reproducibility.
Bioresource Engineering Division RIKEN BioResource Research Center 305 0074 Ibaraki Japan
Communal Laboratory National Center for Global Health and Medicine 162 8655 Tokyo Japan
Faculty of Life and Environmental Sciences University of Tsukuba 305 8572 Ibaraki Japan
School of Medicine Tokai University Isehara 259 1193 Kanagawa Japan
References provided by Crossref.org
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- $a During natural fertilization, mammalian spermatozoa must pass through the zona pellucida before reaching the plasma membrane of the oocyte. It is assumed that this step involves partial lysis of the zona by sperm acrosomal enzymes, but there has been no unequivocal evidence to support this view. Here we present evidence that acrosin, an acrosomal serine protease, plays an essential role in sperm penetration of the zona. We generated acrosin-knockout (KO) hamsters, using an in vivo transfection CRISPR/Cas9 system. Homozygous mutant males were completely sterile. Acrosin-KO spermatozoa ascended the female genital tract and reached ovulated oocytes in the oviduct ampulla, but never fertilized them. In vitro fertilization (IVF) experiments revealed that mutant spermatozoa attached to the zona, but failed to penetrate it. When the zona pellucida was removed before IVF, all oocytes were fertilized. This indicates that in hamsters, acrosin plays an indispensable role in allowing fertilizing spermatozoa to penetrate the zona. This study also suggests that the KO hamster system would be a useful model for identifying new gene functions or analyzing human and animal disorders because of its technical facility and reproducibility.
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