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Acrosin is essential for sperm penetration through the zona pellucida in hamsters
M. Hirose, A. Honda, H. Fulka, M. Tamura-Nakano, S. Matoba, T. Tomishima, K. Mochida, A. Hasegawa, K. Nagashima, K. Inoue, M. Ohtsuka, T. Baba, R. Yanagimachi, A. Ogura,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
Freely Accessible Science Journals od 1915 do Před 6 měsíci
PubMed Central od 1915 do Před 6 měsíci
Europe PubMed Central od 1915 do Před 6 měsíci
Open Access Digital Library od 1915-01-01
Open Access Digital Library od 1915-01-15
Odkazy
PubMed
31964830
DOI
10.1073/pnas.1917595117
Knihovny.cz E-zdroje
- MeSH
- akrosin genetika metabolismus MeSH
- akrozom metabolismus MeSH
- fertilizace in vitro MeSH
- genový knockout MeSH
- interakce spermie a vajíčka * MeSH
- křečci praví genetika metabolismus MeSH
- spermie enzymologie fyziologie MeSH
- zona pellucida metabolismus MeSH
- zvířata MeSH
- Check Tag
- křečci praví genetika metabolismus MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
During natural fertilization, mammalian spermatozoa must pass through the zona pellucida before reaching the plasma membrane of the oocyte. It is assumed that this step involves partial lysis of the zona by sperm acrosomal enzymes, but there has been no unequivocal evidence to support this view. Here we present evidence that acrosin, an acrosomal serine protease, plays an essential role in sperm penetration of the zona. We generated acrosin-knockout (KO) hamsters, using an in vivo transfection CRISPR/Cas9 system. Homozygous mutant males were completely sterile. Acrosin-KO spermatozoa ascended the female genital tract and reached ovulated oocytes in the oviduct ampulla, but never fertilized them. In vitro fertilization (IVF) experiments revealed that mutant spermatozoa attached to the zona, but failed to penetrate it. When the zona pellucida was removed before IVF, all oocytes were fertilized. This indicates that in hamsters, acrosin plays an indispensable role in allowing fertilizing spermatozoa to penetrate the zona. This study also suggests that the KO hamster system would be a useful model for identifying new gene functions or analyzing human and animal disorders because of its technical facility and reproducibility.
Bioresource Engineering Division RIKEN BioResource Research Center 305 0074 Ibaraki Japan
Communal Laboratory National Center for Global Health and Medicine 162 8655 Tokyo Japan
Faculty of Life and Environmental Sciences University of Tsukuba 305 8572 Ibaraki Japan
School of Medicine Tokai University Isehara 259 1193 Kanagawa Japan
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- $a During natural fertilization, mammalian spermatozoa must pass through the zona pellucida before reaching the plasma membrane of the oocyte. It is assumed that this step involves partial lysis of the zona by sperm acrosomal enzymes, but there has been no unequivocal evidence to support this view. Here we present evidence that acrosin, an acrosomal serine protease, plays an essential role in sperm penetration of the zona. We generated acrosin-knockout (KO) hamsters, using an in vivo transfection CRISPR/Cas9 system. Homozygous mutant males were completely sterile. Acrosin-KO spermatozoa ascended the female genital tract and reached ovulated oocytes in the oviduct ampulla, but never fertilized them. In vitro fertilization (IVF) experiments revealed that mutant spermatozoa attached to the zona, but failed to penetrate it. When the zona pellucida was removed before IVF, all oocytes were fertilized. This indicates that in hamsters, acrosin plays an indispensable role in allowing fertilizing spermatozoa to penetrate the zona. This study also suggests that the KO hamster system would be a useful model for identifying new gene functions or analyzing human and animal disorders because of its technical facility and reproducibility.
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- $a Honda, Arata $u Bioresource Engineering Division, RIKEN BioResource Research Center, 305-0074 Ibaraki, Japan. Institute of Laboratory Animals, Kyoto University Graduate School of Medicine, 606-8501 Kyoto, Japan.
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- $a Fulka, Helena $u Laboratory of Epigenetic Regulations, Institute of Molecular Genetics of the ASCR, the Czech Academy of Sciences, Prague 142 20, Czech Republic. Department of Developmental Biology, Institute of Experimental Medicine, The Czech Academy of Sciences, Prague 142 20, Czech Republic.
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