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Activity and inactivity of moth sex chromosomes in somatic and meiotic cells
W. Traut, V. Schubert, M. Daliková, F. Marec, K. Sahara,
Jazyk angličtina Země Rakousko
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
ProQuest Central
od 1997-03-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 1997-03-01 do Před 1 rokem
- MeSH
- bourec genetika metabolismus MeSH
- chromatin metabolismus MeSH
- chromozomy hmyzu metabolismus MeSH
- meióza MeSH
- můry genetika metabolismus MeSH
- pohlavní chromozomy metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Moths and butterflies (Lepidoptera) are the most species-rich group of animals with female heterogamety, females mostly having a WZ, males a ZZ sex chromosome constitution. We studied chromatin conformation, activity, and inactivity of the sex chromosomes in the flour moth Ephestia kuehniella and the silkworm Bombyx mori, using immunostaining with anti-H3K9me2/3, anti-RNA polymerase II, and fluoro-uridine (FU) labelling of nascent transcripts, with conventional widefield fluorescence microscopy and 'spatial structured illumination microscopy' (3D-SIM). The Z chromosome is euchromatic in somatic cells and throughout meiosis. It is transcriptionally active in somatic cells and in the postpachaytene stage of meiosis. The W chromosome in contrast is heterochromatic in somatic cells as well as in meiotic cells at pachytene, but euchromatic and transcriptionally active like all other chromosomes at postpachytene. As the W chromosomes are apparently devoid of protein-coding genes, their transcripts must be non-coding. We found no indication of 'meiotic sex chromosome inactivation' (MSCI) in the two species.
Laboratory of Applied Entomology Faculty of Agriculture Iwate University Morioka 020 8550 Japan
Leibniz Institute of Plant Genetics and Crop Plant Research Gatersleben 06466 Seeland Germany
Citace poskytuje Crossref.org
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