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Repeatability and Reproducibility of the RTgill-W1 Cell Line Assay for Predicting Fish Acute Toxicity
M. Fischer, SE. Belanger, P. Berckmans, MJ. Bernhard, L. Bláha, DE. Coman Schmid, SD. Dyer, T. Haupt, JLM. Hermens, MT. Hultman, H. Laue, A. Lillicrap, M. Mlnaříková, A. Natsch, J. Novák, TL. Sinnige, KE. Tollefsen, V. von Niederhäusern, H....
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
Free Medical Journals
od 1996 do Před 1 rokem
Open Access Digital Library
od 1996-01-01
Medline Complete (EBSCOhost)
od 1998-01-01 do Před 1 rokem
PubMed
30825313
DOI
10.1093/toxsci/kfz057
Knihovny.cz E-zdroje
- MeSH
- aniliny toxicita MeSH
- buněčné linie MeSH
- laboratoře MeSH
- Oncorhynchus mykiss MeSH
- reprodukovatelnost výsledků MeSH
- testy akutní toxicity metody MeSH
- viabilita buněk účinky léků MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Predicting fish acute toxicity of chemicals in vitro is an attractive alternative method to the conventional approach using juvenile and adult fish. The rainbow trout (Oncorhynchus mykiss) cell line assay with RTgill-W1 cells has been designed for this purpose. It quantifies cell viability using fluorescent measurements for metabolic activity, cell- and lysosomal-membrane integrity on the same set of cells. Results from over 70 organic chemicals attest to the high predictive capacity of this test. We here report on the repeatability (intralaboratory variability) and reproducibility (interlaboratory variability) of the RTgill-W1 cell line assay in a round-robin study focusing on 6 test chemicals involving 6 laboratories from the industrial and academic sector. All participating laboratories were able to establish the assay according to preset quality criteria even though, apart from the lead laboratory, none had previously worked with the RTgill-W1 cell line. Concentration-response modeling, based on either nominal or geometric mean-derived measured concentrations, yielded effect concentrations (EC50) that spanned approximately 4 orders of magnitude over the chemical range, covering all fish acute toxicity categories. Coefficients of variation for intralaboratory and interlaboratory variability for the average of the 3 fluorescent cell viability measurements were 15.5% and 30.8%, respectively, which is comparable to other fish-derived, small-scale bioassays. This study therefore underlines the robustness of the RTgill-W1 cell line assay and its accurate performance when carried out by operators in different laboratory settings.
Givaudan Schweiz AG Department of In vitro Molecular Screening 8310 Kemptthal Switzerland
Norwegian Institute for Water Research Section for ecotoxicology 0349 Oslo Norway
RECETOX Faculty of Science Masaryk University 625 00 Brno Bohunice Czech Republic
The Procter and Gamble Company Global Product Stewardship Mason Ohio 45040 USA
Citace poskytuje Crossref.org
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