-
Je něco špatně v tomto záznamu ?
Mid-trimester amniotic fluid proteome's association with spontaneous preterm delivery and gestational duration
M. Hallingström, P. Zedníková, V. Tambor, M. Barman, M. Vajrychová, J. Lenčo, F. Viklund, L. Tancred, H. Rabe, D. Jonsson, A. Kachikis, S. Nilsson, M. Kacerovský, KM. Adams Waldorf, B. Jacobsson,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem
NLK
Directory of Open Access Journals
od 2006
Free Medical Journals
od 2006
Public Library of Science (PLoS)
od 2006
PubMed Central
od 2006
Europe PubMed Central
od 2006
ProQuest Central
od 2006-12-01
Open Access Digital Library
od 2006-01-01
Open Access Digital Library
od 2006-10-01
Open Access Digital Library
od 2006-01-01
Medline Complete (EBSCOhost)
od 2008-01-01
Nursing & Allied Health Database (ProQuest)
od 2006-12-01
Health & Medicine (ProQuest)
od 2006-12-01
Public Health Database (ProQuest)
od 2006-12-01
ROAD: Directory of Open Access Scholarly Resources
od 2006
- MeSH
- amniocentéza MeSH
- dospělí MeSH
- druhý trimestr těhotenství metabolismus MeSH
- gestační stáří * MeSH
- kohortové studie MeSH
- lidé MeSH
- plodová voda chemie metabolismus MeSH
- předčasný porod metabolismus MeSH
- proteom analýza MeSH
- těhotenství MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
BACKGROUND: Amniotic fluid is clinically accessible via amniocentesis and its protein composition may correspond to birth timing. Early changes in the amniotic fluid proteome could therefore be associated with the subsequent development of spontaneous preterm delivery. OBJECTIVE: The main objective of this study was to perform unbiased proteomics analysis of the association between mid-trimester amniotic fluid proteome and spontaneous preterm delivery and gestational duration, respectively. A secondary objective was to validate and replicate the findings by enzyme-linked immunosorbent assay using a second independent cohort. METHODS: Women undergoing a mid-trimester genetic amniocentesis at Sahlgrenska University Hospital/Östra between September 2008 and September 2011 were enrolled in this study, designed in three analytical stages; 1) an unbiased proteomic discovery phase using LC-MS analysis of 22 women with subsequent spontaneous preterm delivery (cases) and 37 women who delivered at term (controls), 2) a validation phase of proteins of interest identified in stage 1, and 3) a replication phase of the proteins that passed validation using a second independent cohort consisting of 20 cases and 40 matched controls. RESULTS: Nine proteins were nominally significantly associated with both spontaneous preterm delivery and gestational duration, after adjustment for gestational age at sampling, but none of the proteins were significant after correction for multiple testing. Several of these proteins have previously been described as being associated with spontaneous PTD etiology and six of them were thus validated using enzyme linked immunosorbent assay. Two of the proteins passed validation; Neutrophil gelatinase-associated lipocalin and plasminogen activator inhibitor 1, but the results could not be replicated in a second cohort. CONCLUSIONS: Neutrophil gelatinase-associated lipocalin and Plasminogen activator inhibitor 1 are potential biomarkers of spontaneous preterm delivery and gestational duration but the findings could not be replicated. The negative findings are supported by the fact that none of the nine proteins from the exploratory phase were significant after correction for multiple testing.
Biomedical Research Center University Hospital Hradec Kralove Hradec Kralove Czech Republic
Department of Obstetrics and Gynecology University of Washington Seattle Washington USA
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc20025021
- 003
- CZ-PrNML
- 005
- 20201222155016.0
- 007
- ta
- 008
- 201125s2020 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1371/journal.pone.0232553 $2 doi
- 035 __
- $a (PubMed)32379834
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Hallingström, Maria $u Department of Obstetrics and Gynecology, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden. Department of Obstetrics and Gynecology, Sahlgrenska University Hospital, Gothenburg, Sweden.
- 245 10
- $a Mid-trimester amniotic fluid proteome's association with spontaneous preterm delivery and gestational duration / $c M. Hallingström, P. Zedníková, V. Tambor, M. Barman, M. Vajrychová, J. Lenčo, F. Viklund, L. Tancred, H. Rabe, D. Jonsson, A. Kachikis, S. Nilsson, M. Kacerovský, KM. Adams Waldorf, B. Jacobsson,
- 520 9_
- $a BACKGROUND: Amniotic fluid is clinically accessible via amniocentesis and its protein composition may correspond to birth timing. Early changes in the amniotic fluid proteome could therefore be associated with the subsequent development of spontaneous preterm delivery. OBJECTIVE: The main objective of this study was to perform unbiased proteomics analysis of the association between mid-trimester amniotic fluid proteome and spontaneous preterm delivery and gestational duration, respectively. A secondary objective was to validate and replicate the findings by enzyme-linked immunosorbent assay using a second independent cohort. METHODS: Women undergoing a mid-trimester genetic amniocentesis at Sahlgrenska University Hospital/Östra between September 2008 and September 2011 were enrolled in this study, designed in three analytical stages; 1) an unbiased proteomic discovery phase using LC-MS analysis of 22 women with subsequent spontaneous preterm delivery (cases) and 37 women who delivered at term (controls), 2) a validation phase of proteins of interest identified in stage 1, and 3) a replication phase of the proteins that passed validation using a second independent cohort consisting of 20 cases and 40 matched controls. RESULTS: Nine proteins were nominally significantly associated with both spontaneous preterm delivery and gestational duration, after adjustment for gestational age at sampling, but none of the proteins were significant after correction for multiple testing. Several of these proteins have previously been described as being associated with spontaneous PTD etiology and six of them were thus validated using enzyme linked immunosorbent assay. Two of the proteins passed validation; Neutrophil gelatinase-associated lipocalin and plasminogen activator inhibitor 1, but the results could not be replicated in a second cohort. CONCLUSIONS: Neutrophil gelatinase-associated lipocalin and Plasminogen activator inhibitor 1 are potential biomarkers of spontaneous preterm delivery and gestational duration but the findings could not be replicated. The negative findings are supported by the fact that none of the nine proteins from the exploratory phase were significant after correction for multiple testing.
- 650 _2
- $a dospělí $7 D000328
- 650 _2
- $a amniocentéza $7 D000649
- 650 _2
- $a plodová voda $x chemie $x metabolismus $7 D000653
- 650 _2
- $a kohortové studie $7 D015331
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 12
- $a gestační stáří $7 D005865
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a těhotenství $7 D011247
- 650 _2
- $a druhý trimestr těhotenství $x metabolismus $7 D011262
- 650 _2
- $a předčasný porod $x metabolismus $7 D047928
- 650 _2
- $a proteom $x analýza $7 D020543
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a Research Support, N.I.H., Extramural $7 D052061
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Zedníková, Petra $u Biomedical Research Center, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic. Department of Biological and Biochemical Science, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic.
- 700 1_
- $a Tambor, Vojtěch $u Biomedical Research Center, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic.
- 700 1_
- $a Barman, Malin $u Department of Biology and Biological Engineering, Food and Nutrition Science, Chalmers University of Technology, Gothenburg, Sweden.
- 700 1_
- $a Vajrychová, Marie $u Biomedical Research Center, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic. Department of Molecular Pathology and Biology, Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic.
- 700 1_
- $a Lenčo, Juraj $u Biomedical Research Center, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic. Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Hradec Kralove, Czech Republic.
- 700 1_
- $a Viklund, Felicia $u Department of Obstetrics and Gynecology, Sahlgrenska University Hospital, Gothenburg, Sweden. Stockholm South General Hospital, Stockholm, Sweden.
- 700 1_
- $a Tancred, Linda $u Department of Obstetrics and Gynecology, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden. Biobank Väst, Sahlgrenska University Hospital, Gothenburg, Sweden.
- 700 1_
- $a Rabe, Hardis $u Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
- 700 1_
- $a Jonsson, Daniel $u Department of Obstetrics and Gynecology, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden. Department of Obstetrics and Gynecology, Sahlgrenska University Hospital, Gothenburg, Sweden.
- 700 1_
- $a Kachikis, Alisa $u Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington, USA.
- 700 1_
- $a Nilsson, Staffan $u Department of Mathematical Sciences, Chalmers University of Technology, Gothenburg, Sweden. Department of Pathology and Genetics, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
- 700 1_
- $a Kacerovský, Marian $u Biomedical Research Center, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic. Department of Obstetrics and Gynecology, Charles University in Prague, Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic.
- 700 1_
- $a Adams Waldorf, Kristina M $u Department of Obstetrics and Gynecology, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden. Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington, USA.
- 700 1_
- $a Jacobsson, Bo $u Department of Obstetrics and Gynecology, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden. Department of Obstetrics and Gynecology, Sahlgrenska University Hospital, Gothenburg, Sweden. Department of Genetics and Bioinformatics, Area of Health Data and Digitalisation, Institute of Public Health, Oslo, Norway.
- 773 0_
- $w MED00180950 $t PloS one $x 1932-6203 $g Roč. 15, č. 5 (2020), s. e0232553
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/32379834 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20201125 $b ABA008
- 991 __
- $a 20201222155012 $b ABA008
- 999 __
- $a ok $b bmc $g 1599166 $s 1115707
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2020 $b 15 $c 5 $d e0232553 $e 20200507 $i 1932-6203 $m PLoS One $n PLoS One $x MED00180950
- LZP __
- $a Pubmed-20201125
