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Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

G. Pramanik, A. Keprova, J. Valenta, V. Bocan, K. Kvaková, L. Libusova, P. Cigler,

. 2020 ; (157) : . [pub] 20200322

Language English Country United States

Document type Journal Article, Research Support, Non-U.S. Gov't, Video-Audio Media

Persistent link   https://www.medvik.cz/link/bmc20025139

Over the past decade, fluorescent gold nanoclusters (AuNCs) have witnessed growing popularity in biological applications and enormous efforts have been devoted to their development. In this protocol, a recently developed, facile method for preparation of water soluble, biocompatible, and colloidally stable near-infrared emitting AuNCs have been described in detail. This room-temperature, bottom-up chemical synthesis provides easily functionalizable AuNCs capped with thioctic acid and thiol-modified polyethylene glycol in aqueous solution. The synthetic approach requires neither organic solvents or additional ligand exchange nor extensive knowledge of synthetic chemistry to reproduce. The resulting AuNCs offer free surface carboxylic acids, which can be functionalized with various biological molecules bearing a free amine group without adversely affecting the photoluminescent properties of the AuNCs. A quick, reliable procedure for flow cytometric quantification and confocal microscopic imaging of AuNC uptake by HeLa cells also been described. Due to the large Stokes shift, proper setting of filters in flow cytometry and confocal microscopy is necessary for efficient detection of near-infrared photoluminescence of AuNCs.

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$a Keprova, Alena $u Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences.
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$a Valenta, Jan $u Department of Chemical Physics and Optics, Faculty of Mathematics and Physics, Charles University.
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$a Bocan, Vaclav $u Department of Cell Biology, Faculty of Science, Charles University.
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$a Kvaková, Klaudia $u Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences; First Faculty of Medicine, Charles University.
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$a Libusova, Lenka $u Department of Cell Biology, Faculty of Science, Charles University.
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$a Cigler, Petr $u Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences; petr.cigler@uochb.cas.cz.
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