• Je něco špatně v tomto záznamu ?

Rapid and reliable HILIC-MS/MS method for monitoring allantoin as a biomarker of oxidative stress

P. Kozlik, L. Hasikova, B. Stiburkova, J. Zavada, K. Kalikova,

. 2020 ; 589 (-) : 113509. [pub] 20191117

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem, validační studie

Perzistentní odkaz   https://www.medvik.cz/link/bmc20025266

Allantoin is an excellent biomarker of oxidative stress in humans as the main product of uric acid oxidation by reactive oxygen species. Yet, allantoin determination is still not routinely performed in clinical laboratories. Therefore, we developed a fast, simple, selective, and sensitive UHPLC-MS/MS method for allantoin determination in human serum using an isotopically labeled internal standard. Our analytical protocol provided high sensitivity by mass spectrometry detection and high throughput by HILIC-MS/MS analysis within 4 min, with one-step serum sample preparation approximately within 7 min. Lastly, our protocol was fully validated to demonstrate its reliability in allantoin determination in human serum. The method showed an excellent linear range from 0.05 to 100 μM, with precision ranging from 1.8 to 11.3% (RSD), and with accuracy (relative error %) within ±6.0%. The method was then applied to analyze the concentration of allantoin in serum samples from 71 patients with chronic gout without treatment with xanthine oxidase inhibitors. The median serum allantoin concentration in the cohort was 2.8 μM (n = 71). Overall, our simple analytical protocol has the potential to be easily implemented in clinical routine practice for monitoring allantoin as a key oxidative stress biomarker.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc20025266
003      
CZ-PrNML
005      
20210217114355.0
007      
ta
008      
201125s2020 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.ab.2019.113509 $2 doi
035    __
$a (PubMed)31747555
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Kozlík, Petr $u Department of Analytical Chemistry, Faculty of Science, Charles University, Prague, Czech Republic. Electronic address: kozlik@natur.cuni.cz. $7 xx0257220
245    10
$a Rapid and reliable HILIC-MS/MS method for monitoring allantoin as a biomarker of oxidative stress / $c P. Kozlik, L. Hasikova, B. Stiburkova, J. Zavada, K. Kalikova,
520    9_
$a Allantoin is an excellent biomarker of oxidative stress in humans as the main product of uric acid oxidation by reactive oxygen species. Yet, allantoin determination is still not routinely performed in clinical laboratories. Therefore, we developed a fast, simple, selective, and sensitive UHPLC-MS/MS method for allantoin determination in human serum using an isotopically labeled internal standard. Our analytical protocol provided high sensitivity by mass spectrometry detection and high throughput by HILIC-MS/MS analysis within 4 min, with one-step serum sample preparation approximately within 7 min. Lastly, our protocol was fully validated to demonstrate its reliability in allantoin determination in human serum. The method showed an excellent linear range from 0.05 to 100 μM, with precision ranging from 1.8 to 11.3% (RSD), and with accuracy (relative error %) within ±6.0%. The method was then applied to analyze the concentration of allantoin in serum samples from 71 patients with chronic gout without treatment with xanthine oxidase inhibitors. The median serum allantoin concentration in the cohort was 2.8 μM (n = 71). Overall, our simple analytical protocol has the potential to be easily implemented in clinical routine practice for monitoring allantoin as a key oxidative stress biomarker.
650    _2
$a alantoin $x krev $7 D000481
650    _2
$a biologické markery $x krev $7 D015415
650    _2
$a vysokoúčinná kapalinová chromatografie $x metody $7 D002851
650    _2
$a chronická nemoc $7 D002908
650    _2
$a kohortové studie $7 D015331
650    _2
$a dna (nemoc) $x metabolismus $7 D006073
650    _2
$a lidé $7 D006801
650    _2
$a oxidační stres $7 D018384
650    _2
$a tandemová hmotnostní spektrometrie $x metody $7 D053719
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
655    _2
$a validační studie $7 D023361
700    1_
$a Hasikova, Lenka $u Institute of Rheumatology, Prague, Czech Republic; Department of Rheumatology, First Faculty of Medicine, Charles University, Prague, Czech Republic.
700    1_
$a Stiburkova, Blanka $u Institute of Rheumatology, Prague, Czech Republic; Department of Pediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech Republic.
700    1_
$a Zavada, Jakub $u Institute of Rheumatology, Prague, Czech Republic; Department of Rheumatology, First Faculty of Medicine, Charles University, Prague, Czech Republic.
700    1_
$a Kalikova, Kveta $u Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University, Prague, Czech Republic.
773    0_
$w MED00000335 $t Analytical biochemistry $x 1096-0309 $g Roč. 589, č. - (2020), s. 113509
856    41
$u https://pubmed.ncbi.nlm.nih.gov/31747555 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20201125 $b ABA008
991    __
$a 20210217114309 $b ABA008
999    __
$a ok $b bmc $g 1599411 $s 1115952
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 589 $c - $d 113509 $e 20191117 $i 1096-0309 $m Analytical biochemistry $n Anal Biochem $x MED00000335
LZP    __
$a Pubmed-20201125

Najít záznam

Citační ukazatele

Možnosti archivace