Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Bacterial immunogenic α-galactosylceramide identified in the murine large intestine: dependency on diet and inflammation

J. von Gerichten, D. Lamprecht, L. Opálka, D. Soulard, C. Marsching, R. Pilz, V. Sencio, S. Herzer, B. Galy, V. Nordström, C. Hopf, HJ. Gröne, F. Trottein, R. Sandhoff,

. 2019 ; 60 (11) : 1892-1904. [pub] 20190904

Language English Country United States

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc20025544

The glycosphingolipid, α-galactosylceramide (αGalCer), when presented by CD1d on antigen-presenting cells, efficiently activates invariant natural killer T (iNKT) cells. Thereby, it modulates immune responses against tumors, microbial and viral infections, and autoimmune diseases. Recently, the production of αGalCer by Bacteroidetes from the human gut microbiome was elucidated. Using hydrophilic interaction chromatography coupled to MS2, we screened murine intestinal tracts to identify and quantify αGalCers, and we investigated the αGalCer response to different dietary and physiologic conditions. In both the cecum and the colon of mice, we found 1-15 pmol of αGalCer per milligram of protein; in contrast, mice lacking microbiota (germ-free mice) and fed identical diet did not harbor αGalCer. The identified αGalCer contained a β(R)-hydroxylated hexadecanoyl chain N-linked to C18-sphinganine, which differed from what has been reported with Bacteroides fragilis Unlike β-anomeric structures, but similar to αGalCers from B. fragilis, the synthetic form of the murine αGalCer induced iNKT cell activation in vitro. Last, we observed a decrease in αGalCer production in mice exposed to conditions that alter the composition of the gut microbiota, including Western type diet, colitis, and influenza A virus infection. Collectively, this study suggests that αGalCer is produced by commensals in the mouse intestine and reveals that stressful conditions causing dysbiosis alter its synthesis. The consequences of this altered production on iNKT cell-mediated local and systemic immune responses are worthy of future studies.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc20025544
003      
CZ-PrNML
005      
20201222155245.0
007      
ta
008      
201125s2019 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1194/jlr.RA119000236 $2 doi
035    __
$a (PubMed)31484693
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a von Gerichten, Johanna $u Lipid Pathobiochemistry Group, Department of Cellular and Molecular Pathology, German Cancer Research Center, Heidelberg, Germany. Faculty of Biosciences, University of Heidelberg, Heidelberg, Germany.
245    10
$a Bacterial immunogenic α-galactosylceramide identified in the murine large intestine: dependency on diet and inflammation / $c J. von Gerichten, D. Lamprecht, L. Opálka, D. Soulard, C. Marsching, R. Pilz, V. Sencio, S. Herzer, B. Galy, V. Nordström, C. Hopf, HJ. Gröne, F. Trottein, R. Sandhoff,
520    9_
$a The glycosphingolipid, α-galactosylceramide (αGalCer), when presented by CD1d on antigen-presenting cells, efficiently activates invariant natural killer T (iNKT) cells. Thereby, it modulates immune responses against tumors, microbial and viral infections, and autoimmune diseases. Recently, the production of αGalCer by Bacteroidetes from the human gut microbiome was elucidated. Using hydrophilic interaction chromatography coupled to MS2, we screened murine intestinal tracts to identify and quantify αGalCers, and we investigated the αGalCer response to different dietary and physiologic conditions. In both the cecum and the colon of mice, we found 1-15 pmol of αGalCer per milligram of protein; in contrast, mice lacking microbiota (germ-free mice) and fed identical diet did not harbor αGalCer. The identified αGalCer contained a β(R)-hydroxylated hexadecanoyl chain N-linked to C18-sphinganine, which differed from what has been reported with Bacteroides fragilis Unlike β-anomeric structures, but similar to αGalCers from B. fragilis, the synthetic form of the murine αGalCer induced iNKT cell activation in vitro. Last, we observed a decrease in αGalCer production in mice exposed to conditions that alter the composition of the gut microbiota, including Western type diet, colitis, and influenza A virus infection. Collectively, this study suggests that αGalCer is produced by commensals in the mouse intestine and reveals that stressful conditions causing dysbiosis alter its synthesis. The consequences of this altered production on iNKT cell-mediated local and systemic immune responses are worthy of future studies.
650    _2
$a zvířata $7 D000818
650    _2
$a Bacteroides fragilis $x chemie $x imunologie $7 D001441
650    12
$a dieta $7 D004032
650    _2
$a galaktosylceramidy $x genetika $x imunologie $7 D005699
650    _2
$a zánět $x imunologie $x mikrobiologie $7 D007249
650    _2
$a tlusté střevo $x imunologie $x metabolismus $x mikrobiologie $7 D007420
650    _2
$a myši $7 D051379
650    _2
$a inbrední kmeny myší $7 D008815
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Lamprecht, Dominic $u Lipid Pathobiochemistry Group, Department of Cellular and Molecular Pathology, German Cancer Research Center, Heidelberg, Germany.
700    1_
$a Opálka, Lukáš $u Lipid Pathobiochemistry Group, Department of Cellular and Molecular Pathology, German Cancer Research Center, Heidelberg, Germany. Skin Barrier Research Group, Department of Organic and Bioorganic Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Hradec Králové, Czech Republic.
700    1_
$a Soulard, Daphnée $u Centre d'Infection et d'Immunité de Lille, Inserm U1019, CNRS UMR 8204, University of Lille, CHU Lille, Institut Pasteur de Lille, Lille, France.
700    1_
$a Marsching, Christian $u Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences, Mannheim, Germany.
700    1_
$a Pilz, Robert $u Lipid Pathobiochemistry Group, Department of Cellular and Molecular Pathology, German Cancer Research Center, Heidelberg, Germany. Faculty of Biosciences, University of Heidelberg, Heidelberg, Germany.
700    1_
$a Sencio, Valentin $u Centre d'Infection et d'Immunité de Lille, Inserm U1019, CNRS UMR 8204, University of Lille, CHU Lille, Institut Pasteur de Lille, Lille, France.
700    1_
$a Herzer, Silke $u Department of Cellular and Molecular Pathology, German Cancer Research Center, Heidelberg, Germany.
700    1_
$a Galy, Bruno $u Division of Virus-Associated Carcinogenesis, German Cancer Research Center, Heidelberg, Germany.
700    1_
$a Nordström, Viola $u Department of Cellular and Molecular Pathology, German Cancer Research Center, Heidelberg, Germany.
700    1_
$a Hopf, Carsten $u Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences, Mannheim, Germany.
700    1_
$a Gröne, Hermann-Josef $u Department of Cellular and Molecular Pathology, German Cancer Research Center, Heidelberg, Germany. Institute of Pharmacology, University of Marburg, Marburg, Germany.
700    1_
$a Trottein, François $u Centre d'Infection et d'Immunité de Lille, Inserm U1019, CNRS UMR 8204, University of Lille, CHU Lille, Institut Pasteur de Lille, Lille, France.
700    1_
$a Sandhoff, Roger $u Lipid Pathobiochemistry Group, Department of Cellular and Molecular Pathology, German Cancer Research Center, Heidelberg, Germany r.sandhoff@dkfz.de.
773    0_
$w MED00002766 $t Journal of lipid research $x 1539-7262 $g Roč. 60, č. 11 (2019), s. 1892-1904
856    41
$u https://pubmed.ncbi.nlm.nih.gov/31484693 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20201125 $b ABA008
991    __
$a 20201222155241 $b ABA008
999    __
$a ok $b bmc $g 1599689 $s 1116230
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2019 $b 60 $c 11 $d 1892-1904 $e 20190904 $i 1539-7262 $m Journal of lipid research $n J Lipid Res $x MED00002766
LZP    __
$a Pubmed-20201125

Find record

Citation metrics

Archiving options