Wound dressings with silver have been shown to be cytotoxic in vitro. However, the extrapolation of this cytotoxicity to clinical settings is unclear. We applied dressings with various forms of silver on porcine skin ex vivo and investigated silver penetration and DNA damage. We assessed antimicrobial efficacy, cytotoxicity to skin cells, and immune response induced by the dressings. All dressings elevated the DNA damage marker γ-H2AX and the expression of stress-related genes in explanted skin relative to control. This corresponded with the amount of silver in the skin. The dressings reduced viability, induced oxidative stress and DNA damage in skin cells, and induced the production of pro-inflammatory IL-6 by monocytes. The oxidative burst and viability of activated neutrophils decreased. The amount of silver released into the culture medium varied among the dressings and correlated with in vitro toxicity. However, antimicrobial efficiencies did not correlate strongly with the amount of silver released from the dressings. Antimicrobial efficiency and toxicity are driven by the form of silver and the construction of dressings and not only by the silver concentration. The damaging effects of silver dressings in ex vivo skin highlight the importance of thorough in vivo investigation of silver dressing toxicity.

Contipro a s Dolni Dobrouc 401 56102 Dolni Dobrouc Czech Republic

Contipro a s Dolni Dobrouc 401 56102 Dolni Dobrouc Czech Republic 3rd Faculty of Medicine Charles University Prague Czech Republic

Contipro a s Dolni Dobrouc 401 56102 Dolni Dobrouc Czech Republic Faculty of Natural Sciences Charles University Prague Czech Republic

Department of Biophysics of Immune System Institute of Biophysics of the Czech Academy of Sciences Brno Czech Republic Department of Experimental Biology Faculty of Science Masaryk University Brno Czech Republic

Department of Biophysics of Immune System Institute of Biophysics of the Czech Academy of Sciences Brno Czech Republic Department of Experimental Biology Faculty of Science Masaryk University Brno Czech Republic International Clinical Research Center St Anne's University Hospital Brno Czech Republic

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$a Effects of wound dressings containing silver on skin and immune cells / $c K. Nešporová, V. Pavlík, B. Šafránková, H. Vágnerová, P. Odráška, O. Žídek, N. Císařová, S. Skoroplyas, L. Kubala, V. Velebný,

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$a Wound dressings with silver have been shown to be cytotoxic in vitro. However, the extrapolation of this cytotoxicity to clinical settings is unclear. We applied dressings with various forms of silver on porcine skin ex vivo and investigated silver penetration and DNA damage. We assessed antimicrobial efficacy, cytotoxicity to skin cells, and immune response induced by the dressings. All dressings elevated the DNA damage marker γ-H2AX and the expression of stress-related genes in explanted skin relative to control. This corresponded with the amount of silver in the skin. The dressings reduced viability, induced oxidative stress and DNA damage in skin cells, and induced the production of pro-inflammatory IL-6 by monocytes. The oxidative burst and viability of activated neutrophils decreased. The amount of silver released into the culture medium varied among the dressings and correlated with in vitro toxicity. However, antimicrobial efficiencies did not correlate strongly with the amount of silver released from the dressings. Antimicrobial efficiency and toxicity are driven by the form of silver and the construction of dressings and not only by the silver concentration. The damaging effects of silver dressings in ex vivo skin highlight the importance of thorough in vivo investigation of silver dressing toxicity.

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$a Pavlík, Vojtěch $u Contipro a.s., Dolni Dobrouc 401, 56102, Dolni Dobrouc, Czech Republic. Third Faculty of Medicine, Charles University, Prague, Czech Republic.

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$a Císařová, Natálie $u Contipro a.s., Dolni Dobrouc 401, 56102, Dolni Dobrouc, Czech Republic. Faculty of Natural Sciences, Charles University, Prague, Czech Republic.

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$a Skoroplyas, Svitlana $u Department of Biophysics of Immune System, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic. Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.

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$a Kubala, Lukáš $u Department of Biophysics of Immune System, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic. Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic. International Clinical Research Center, St. Anne's University Hospital, Brno, Czech Republic.

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