Being a chilling-sensitive staple crop, rice (Oryza sativa L.) is vulnerable to climate change. The competence of rice to withstand chilling stress should, therefore, be enhanced through technological tools. The present study employed chemical intervention like application of sodium nitroprusside (SNP) as nitric oxide (NO) donor and elucidated the underlying morpho-physiological and biochemical mechanisms of NO-mediated chilling tolerance in rice plants. At germination stage, germination indicators were interrupted by chilling stress (5.0 ± 1.0 °C for 8 h day-1), while pretreatment with 100 μM SNP markedly improved all the indicators. At seedling stage (14-day-old), chilling stress caused stunted growth with visible toxicity along with alteration of biochemical markers, for example, increase in oxidative stress markers (superoxide, hydrogen peroxide, and malondialdehyde) and osmolytes (total soluble sugar; proline and soluble protein content, SPC), and decrease in chlorophyll (Chl), relative water content (RWC), and antioxidants. However, NO application attenuated toxicity symptoms with improving growth attributes which might be related to enhance activities of antioxidants, mineral contents, Chl, RWC and SPC. Furthermore, principal component analysis indicated that water imbalance and increased oxidative damage were the main contributors to chilling injury, whereas NO-mediated mineral homeostasis and antioxidant defense were the critical determinants for chilling tolerance in rice. Collectively, our findings revealed that NO protects against chilling stress through valorizing cellular defense mechanisms, suggesting that exogenous application of NO could be a potential tool to evolve cold tolerance as well as climate resilience in rice.

Department of Biochemistry and Molecular Biology Faculty of Agriculture Bangladesh Agricultural University Mymensingh 2202 Bangladesh

Department of Biochemistry and Molecular Biology Shahjalal University of Science and Technology Sylhet 3114 Bangladesh

Department of Botany and Plant Physiology Faculty of Agrobiology Food and Natural Resources Czech University of Life Sciences 16500 Prague Czech Republic

Department of Plant Physiology Faculty of Agrobiology and Food Resources Slovak University of Agriculture 94976 Nitra Slovakia

Graduate School of Environmental and Life Science Okayama University Kita ku Okayama 700 8530 Japan

000      

00000naa a2200000 a 4500

001      

bmc20027989

003      

CZ-PrNML

005      

20210114152742.0

007      

ta

008      

210105s2020 xxu f 000 0|eng||

009      

AR

024    7_

$a 10.1016/j.niox.2020.04.001 $2 doi

035    __

$a (PubMed)32283262

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a xxu

100    1_

$a Sohag, Abdullah Al Mamun $u Department of Biochemistry and Molecular Biology, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh. Electronic address: sohag2010bmb.sust@gmail.com.

245    10

$a Insights into nitric oxide-mediated water balance, antioxidant defence and mineral homeostasis in rice (Oryza sativa L.) under chilling stress / $c AAM. Sohag, M. Tahjib-Ul-Arif, S. Afrin, MK. Khan, MA. Hannan, M. Skalicky, MG. Mortuza, M. Brestic, MA. Hossain, Y. Murata,

520    9_

$a Being a chilling-sensitive staple crop, rice (Oryza sativa L.) is vulnerable to climate change. The competence of rice to withstand chilling stress should, therefore, be enhanced through technological tools. The present study employed chemical intervention like application of sodium nitroprusside (SNP) as nitric oxide (NO) donor and elucidated the underlying morpho-physiological and biochemical mechanisms of NO-mediated chilling tolerance in rice plants. At germination stage, germination indicators were interrupted by chilling stress (5.0 ± 1.0 °C for 8 h day-1), while pretreatment with 100 μM SNP markedly improved all the indicators. At seedling stage (14-day-old), chilling stress caused stunted growth with visible toxicity along with alteration of biochemical markers, for example, increase in oxidative stress markers (superoxide, hydrogen peroxide, and malondialdehyde) and osmolytes (total soluble sugar; proline and soluble protein content, SPC), and decrease in chlorophyll (Chl), relative water content (RWC), and antioxidants. However, NO application attenuated toxicity symptoms with improving growth attributes which might be related to enhance activities of antioxidants, mineral contents, Chl, RWC and SPC. Furthermore, principal component analysis indicated that water imbalance and increased oxidative damage were the main contributors to chilling injury, whereas NO-mediated mineral homeostasis and antioxidant defense were the critical determinants for chilling tolerance in rice. Collectively, our findings revealed that NO protects against chilling stress through valorizing cellular defense mechanisms, suggesting that exogenous application of NO could be a potential tool to evolve cold tolerance as well as climate resilience in rice.

650    _2

$a nízká teplota $7 D003080

650    _2

$a reakce na chladový šok $x fyziologie $7 D058639

650    _2

$a ochrana úrody $x metody $7 D000069601

650    _2

$a klíčení $x účinky léků $7 D018525

650    _2

$a homeostáza $x účinky léků $7 D006706

650    _2

$a peroxid vodíku $x metabolismus $7 D006861

650    _2

$a peroxidace lipidů $x účinky léků $7 D015227

650    _2

$a malondialdehyd $x metabolismus $7 D008315

650    _2

$a oxid dusnatý $x metabolismus $7 D009569

650    _2

$a donory oxidu dusnatého $x farmakologie $7 D020030

650    _2

$a nitroprusid $x farmakologie $7 D009599

650    _2

$a rýže (rod) $x účinky léků $7 D012275

650    _2

$a peroxidasy $x metabolismus $7 D010544

650    _2

$a semenáček $x účinky léků $7 D036226

650    _2

$a superoxidy $x metabolismus $7 D013481

655    _2

$a časopisecké články $7 D016428

655    _2

$a práce podpořená grantem $7 D013485

700    1_

$a Tahjib-Ul-Arif, Md $u Department of Biochemistry and Molecular Biology, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh; Graduate School of Environmental and Life Science, Okayama University, Kita-ku, Okayama, 700-8530, Japan. Electronic address: tahjib@bau.edu.bd.

700    1_

$a Afrin, Sonya $u Graduate School of Environmental and Life Science, Okayama University, Kita-ku, Okayama, 700-8530, Japan. Electronic address: sonya.bau288@gmail.com.

700    1_

$a Khan, Md Kawsar $u Department of Biochemistry and Molecular Biology, Shahjalal University of Science & Technology, Sylhet, 3114, Bangladesh. Electronic address: bmbkawsar@gmail.com.

700    1_

$a Hannan, Md Abdul $u Department of Biochemistry and Molecular Biology, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh. Electronic address: hannanbmb@bau.edu.bd.

700    1_

$a Skalicky, Milan $u Department of Botany and Plant Physiology, Faculty of Agrobiology, Food, and Natural Resources, Czech University of Life Sciences, 16500, Prague, Czech Republic. Electronic address: skalicky@af.czu.cz.

700    1_

$a Mortuza, Md Golam $u Department of Biochemistry and Molecular Biology, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh. Electronic address: gmortuza2003@yahoo.com.

700    1_

$a Brestic, Marian $u Department of Botany and Plant Physiology, Faculty of Agrobiology, Food, and Natural Resources, Czech University of Life Sciences, 16500, Prague, Czech Republic; Department of Plant Physiology, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture, 94976, Nitra, Slovakia. Electronic address: marian.brestic@uniag.sk.

700    1_

$a Hossain, M Afzal $u Department of Biochemistry and Molecular Biology, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh. Electronic address: mafzal.bau@gmail.com.

700    1_

$a Murata, Yoshiyuki $u Graduate School of Environmental and Life Science, Okayama University, Kita-ku, Okayama, 700-8530, Japan. Electronic address: muta@cc.okayama-u.ac.jp.

773    0_

$w MED00004882 $t Nitric oxide : biology and chemistry $x 1089-8611 $g Roč. 100-101, č. - (2020), s. 7-16

856    41

$u https://pubmed.ncbi.nlm.nih.gov/32283262 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y a $z 0

990    __

$a 20210105 $b ABA008

991    __

$a 20210114152740 $b ABA008

999    __

$a ok $b bmc $g 1608324 $s 1119169

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2020 $b 100-101 $c - $d 7-16 $e 20200410 $i 1089-8611 $m Nitric oxide $n Nitric oxide $x MED00004882

LZP    __

$a Pubmed-20210105