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Modulation of non-bilayer lipid phases and the structure and functions of thylakoid membranes: effects on the water-soluble enzyme violaxanthin de-epoxidase

O. Dlouhý, I. Kurasová, V. Karlický, U. Javornik, P. Šket, NZ. Petrova, SB. Krumova, J. Plavec, B. Ughy, V. Špunda, G. Garab,

. 2020 ; 10 (1) : 11959. [pub] 20200720

Language English Country Great Britain

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc20028010

The role of non-bilayer lipids and non-lamellar lipid phases in biological membranes is an enigmatic problem of membrane biology. Non-bilayer lipids are present in large amounts in all membranes; in energy-converting membranes they constitute about half of their total lipid content-yet their functional state is a bilayer. In vitro experiments revealed that the functioning of the water-soluble violaxanthin de-epoxidase (VDE) enzyme of plant thylakoids requires the presence of a non-bilayer lipid phase. 31P-NMR spectroscopy has provided evidence on lipid polymorphism in functional thylakoid membranes. Here we reveal reversible pH- and temperature-dependent changes of the lipid-phase behaviour, particularly the flexibility of isotropic non-lamellar phases, of isolated spinach thylakoids. These reorganizations are accompanied by changes in the permeability and thermodynamic parameters of the membranes and appear to control the activity of VDE and the photoprotective mechanism of non-photochemical quenching of chlorophyll-a fluorescence. The data demonstrate, for the first time in native membranes, the modulation of the activity of a water-soluble enzyme by a non-bilayer lipid phase.

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$a The role of non-bilayer lipids and non-lamellar lipid phases in biological membranes is an enigmatic problem of membrane biology. Non-bilayer lipids are present in large amounts in all membranes; in energy-converting membranes they constitute about half of their total lipid content-yet their functional state is a bilayer. In vitro experiments revealed that the functioning of the water-soluble violaxanthin de-epoxidase (VDE) enzyme of plant thylakoids requires the presence of a non-bilayer lipid phase. 31P-NMR spectroscopy has provided evidence on lipid polymorphism in functional thylakoid membranes. Here we reveal reversible pH- and temperature-dependent changes of the lipid-phase behaviour, particularly the flexibility of isotropic non-lamellar phases, of isolated spinach thylakoids. These reorganizations are accompanied by changes in the permeability and thermodynamic parameters of the membranes and appear to control the activity of VDE and the photoprotective mechanism of non-photochemical quenching of chlorophyll-a fluorescence. The data demonstrate, for the first time in native membranes, the modulation of the activity of a water-soluble enzyme by a non-bilayer lipid phase.
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$a Kurasová, Irena $u Faculty of Science, University of Ostrava, Ostrava, Czech Republic. Global Change Research Institute, Czech Academy of Sciences, Brno, Czech Republic.
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$a Karlický, Václav $u Faculty of Science, University of Ostrava, Ostrava, Czech Republic. Global Change Research Institute, Czech Academy of Sciences, Brno, Czech Republic.
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$a Javornik, Uroš $u Slovenian NMR Center, National Institute of Chemistry, Ljubljana, Slovenia.
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$a Šket, Primož $u Slovenian NMR Center, National Institute of Chemistry, Ljubljana, Slovenia. EN-FIST Center of Excellence, Ljubljana, Slovenia.
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$a Petrova, Nia Z $u Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia, Bulgaria.
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$a Krumova, Sashka B $u Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia, Bulgaria.
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$a Plavec, Janez $u Slovenian NMR Center, National Institute of Chemistry, Ljubljana, Slovenia. EN-FIST Center of Excellence, Ljubljana, Slovenia. Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ljubljana, Slovenia.
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$a Ughy, Bettina $u Faculty of Science, University of Ostrava, Ostrava, Czech Republic. ughy.bettina@brc.hu. Institute of Plant Biology, Biological Research Centre, Szeged, Hungary. ughy.bettina@brc.hu.
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$a Špunda, Vladimír $u Faculty of Science, University of Ostrava, Ostrava, Czech Republic. vladimir.spunda@osu.cz. Global Change Research Institute, Czech Academy of Sciences, Brno, Czech Republic. vladimir.spunda@osu.cz.
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$a Garab, Győző $u Faculty of Science, University of Ostrava, Ostrava, Czech Republic. garab.gyozo@brc.hu. Institute of Plant Biology, Biological Research Centre, Szeged, Hungary. garab.gyozo@brc.hu.
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