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Genes regulating hormone stimulus and response to protein signaling revealed differential expression pattern during porcine oocyte in vitro maturation, confirmed by lipid concentration
B. Chermuła, M. Jeseta, P. Sujka-Kordowska, A. Konwerska, M. Jankowski, W. Kranc, I. Kocherova, P. Celichowski, P. Antosik, D. Bukowska, I. Milakovic, M. Machatkova, L. Pawelczyk, D. Iżycki, M. Zabel, P. Mozdziak, B. Kempisty, H. Piotrowska-Kempisty,
Language English Country Germany
Document type Journal Article
Grant support
FNBr, 65269705
Ministry of Health, Czech Republic
NLK
ProQuest Central
from 1997-01-01 to 1 year ago
Medline Complete (EBSCOhost)
from 2000-01-01 to 1 year ago
Nursing & Allied Health Database (ProQuest)
from 1997-01-01 to 1 year ago
Health & Medicine (ProQuest)
from 1997-01-01 to 1 year ago
Public Health Database (ProQuest)
from 1997-01-01 to 1 year ago
- MeSH
- Eosine Yellowish-(YS) chemistry MeSH
- Hematoxylin chemistry MeSH
- Hormones genetics metabolism MeSH
- In Vitro Oocyte Maturation Techniques * MeSH
- Cells, Cultured MeSH
- Lipids analysis MeSH
- Oocytes growth & development metabolism MeSH
- Oxazines chemistry MeSH
- Swine MeSH
- Signal Transduction MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Genes influencing oocyte maturation may be valuable for predicting their developmental potential, as well as discerning the mechanistic pathways regulating oocyte development. In the presented research microarray gene expression analysis of immature and in vitro matured porcine oocytes was performed. Two groups of oocytes were compared in the study: before (3 × n = 50) and after in vitro maturation (3 × n = 50). The selection of viable oocytes was performed using the brilliant cresyl blue (BCB) test. Furthermore, microarrays and RT-qPCR was used to analyze the transcriptome of the oocytes before and after IVM. The study focused on the genes undergoing differential expression in two gene-ontology groups: "Cellular response to hormone stimulus" and "Cellular response to unfolded protein", which contain genes that may directly or indirectly be involved in signal transduction during oocyte maturation. Examination of all the genes of interest showed a lower level of their expression after IVM. From the total number of genes in these gene ontologies ten of the highest change in expression were identified: FOS, ID2, BTG2, CYR61, ESR1, AR, TACR3, CCND2, EGR2 and TGFBR3. The successful maturation of the oocytes was additionally confirmed with the use of lipid droplet assay. The genes were briefly described and related to the literature sources, to investigate their potential roles in the process of oocyte maturation. The results of the study may serve as a basic molecular reference for further research aimed at improving the methods of oocyte in vitro maturation, which plays an important role in the procedures of assisted reproduction.
Department of Anatomy Poznan University of Medical Sciences Poznan Poland
Department of Toxicology Poznan University of Medical Sciences Poznan Poland
Department of Veterinary Surgery Nicolaus Copernicus University in Torun Toruń Poland
Physiology Graduate Program North Carolina State University Raleigh NC USA
References provided by Crossref.org
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- $a Chermuła, Błażej $u Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, Poznan, Poland.
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- $a Genes regulating hormone stimulus and response to protein signaling revealed differential expression pattern during porcine oocyte in vitro maturation, confirmed by lipid concentration / $c B. Chermuła, M. Jeseta, P. Sujka-Kordowska, A. Konwerska, M. Jankowski, W. Kranc, I. Kocherova, P. Celichowski, P. Antosik, D. Bukowska, I. Milakovic, M. Machatkova, L. Pawelczyk, D. Iżycki, M. Zabel, P. Mozdziak, B. Kempisty, H. Piotrowska-Kempisty,
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- $a Genes influencing oocyte maturation may be valuable for predicting their developmental potential, as well as discerning the mechanistic pathways regulating oocyte development. In the presented research microarray gene expression analysis of immature and in vitro matured porcine oocytes was performed. Two groups of oocytes were compared in the study: before (3 × n = 50) and after in vitro maturation (3 × n = 50). The selection of viable oocytes was performed using the brilliant cresyl blue (BCB) test. Furthermore, microarrays and RT-qPCR was used to analyze the transcriptome of the oocytes before and after IVM. The study focused on the genes undergoing differential expression in two gene-ontology groups: "Cellular response to hormone stimulus" and "Cellular response to unfolded protein", which contain genes that may directly or indirectly be involved in signal transduction during oocyte maturation. Examination of all the genes of interest showed a lower level of their expression after IVM. From the total number of genes in these gene ontologies ten of the highest change in expression were identified: FOS, ID2, BTG2, CYR61, ESR1, AR, TACR3, CCND2, EGR2 and TGFBR3. The successful maturation of the oocytes was additionally confirmed with the use of lipid droplet assay. The genes were briefly described and related to the literature sources, to investigate their potential roles in the process of oocyte maturation. The results of the study may serve as a basic molecular reference for further research aimed at improving the methods of oocyte in vitro maturation, which plays an important role in the procedures of assisted reproduction.
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