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Generation and characterization of Aldh3-Cre transgenic mice as a tool for conditional gene deletion in postnatal cornea
SS. Sunny, J. Lachova, N. Dupacova, A. Zitova, Z. Kozmik,
Jazyk angličtina Země Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
Directory of Open Access Journals
od 2011
Free Medical Journals
od 2011
PubMed Central
od 2011
Europe PubMed Central
od 2011
Open Access Digital Library
od 2011-01-01
Open Access Digital Library
od 2011-01-01
ROAD: Directory of Open Access Scholarly Resources
od 2011
Springer Nature OA/Free Journals
od 2011-12-01
Springer Nature - nature.com Journals - Fully Open Access
od 2011-12-01
- MeSH
- aldehyddehydrogenasa genetika MeSH
- alely MeSH
- delece genu MeSH
- epitelové buňky fyziologie MeSH
- genový targeting metody MeSH
- integrasy genetika MeSH
- konjunktiva fyziologie MeSH
- myši transgenní genetika fyziologie MeSH
- myši MeSH
- rohovka fyziologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Conditional gene targeting in mice by means of Cre-loxP strategy represents a powerful approach to study mammalian gene function. This approach is however dependent on the availability of suitable strains of mice with a tissue or time restricted activity of the Cre recombinase. Here we describe Aldh3-Cre transgenic mice as a useful tool to conditionally delete genes in cornea, a specialized transparent tissue found on the anterior-most part of the eye, which acts as a protective barrier and contributes to the refractive power. Using a set of floxed alleles we demonstrate high Aldh3-Cre activity in corneal epithelial cells, corneal stroma and conjunctival epithelial cells at postnatal stages. Aldh3-Cre will thus be particularly beneficial for functional analysis of genes which are vital for postnatal development of cornea and conjunctiva.
Citace poskytuje Crossref.org
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