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Chemopreventive and Therapeutic Efficacy of Cinnamomum zeylanicum L. Bark in Experimental Breast Carcinoma: Mechanistic In Vivo and In Vitro Analyses
P. Kubatka, M. Kello, K. Kajo, M. Samec, K. Jasek, D. Vybohova, S. Uramova, A. Liskova, V. Sadlonova, L. Koklesova, R. Murin, M. Adamkov, K. Smejkal, E. Svajdlenka, P. Solar, SM. Samuel, M. Kassayova, TK. Kwon, P. Zubor, M. Pec, J. Danko, D....
Language English Country Switzerland
Document type Journal Article
Grant support
1/0136/19, 1/0653/19, and 1/0753/17
Vedecká Grantová Agentúra MŠVVaŠ SR a SAV
APVV-16-0021 and APVV-16-0446
Agentúra na Podporu Výskumu a Vývoja
code ITMS2014 + 313011D103
"Medicínsky univerzitný vedecký park v Košiciach (MediPark, Košice - Fáza II.)",
ITMS: 26220120053
"CENTER OF EXCELLENCE FOR RESEARCH IN PERSONALIZED THERAPY (CEVYPET)",
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- MeSH
- Antineoplastic Agents, Phytogenic administration & dosage chemistry pharmacology MeSH
- Histones metabolism MeSH
- Rats MeSH
- Plant Bark chemistry MeSH
- Humans MeSH
- MCF-7 Cells MeSH
- MicroRNAs genetics MeSH
- Mice MeSH
- Cell Line, Tumor MeSH
- Breast Neoplasms drug therapy genetics metabolism MeSH
- Oils, Volatile administration & dosage chemistry pharmacology MeSH
- Plant Oils administration & dosage chemistry pharmacology MeSH
- Cell Proliferation drug effects MeSH
- Cinnamomum zeylanicum chemistry MeSH
- Cell Survival drug effects MeSH
- Dose-Response Relationship, Drug MeSH
- Xenograft Model Antitumor Assays MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Humans MeSH
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Comprehensive oncology research suggests an important role of phytochemicals or whole plant foods in the modulation of signaling pathways associated with anticancer action. The goal of this study is to assess the anticancer activities of Cinnamomum zeylanicum L. using rat, mouse, and cell line breast carcinoma models. C. zeylanicum (as bark powder) was administered in the diet at two concentrations of 0.1% (w/w) and 1% (w/w) during the whole experiment in chemically induced rat mammary carcinomas and a syngeneic 4T1 mouse model. After autopsy, histopathological and molecular evaluations of mammary gland tumors in rodents were carried out. Moreover, in vitro analyses using MCF-7 and MDA-MB-231 cells were performed. The dominant metabolites present in the tested C. zeylanicum essential oil (with relative content over 1%) were cinnamaldehyde, cinnamaldehyde dimethyl acetal, cinnamyl acetate, eugenol, linalool, eucalyptol, limonene, o-cymol, and α-terpineol. The natural mixture of mentioned molecules demonstrated significant anticancer effects in our study. In the mouse model, C. zeylanicum at a higher dose (1%) significantly decreased tumor volume by 44% when compared to controls. In addition, treated tumors showed a significant dose-dependent decrease in mitotic activity index by 29% (0.1%) and 45.5% (1%) in comparison with the control group. In rats, C. zeylanicum in both doses significantly reduced the tumor incidence by 15.5% and non-significantly suppressed tumor frequency by more than 30% when compared to controls. An evaluation of the mechanism of anticancer action using valid oncological markers showed several positive changes after treatment with C. zeylanicum. Histopathological analysis of treated rat tumor specimens showed a significant decrease in the ratio of high-/low-grade carcinomas compared to controls. In treated rat carcinomas, we found caspase-3 and Bax expression increase. On the other hand, we observed a decrease in Bcl-2, Ki67, VEGF, and CD24 expressions and MDA levels. Assessment of epigenetic changes in rat tumor cells in vivo showed a significant decrease in lysine methylation status of H3K4m3 and H3K9m3 in the high-dose treated group, a dose-dependent increase in H4K16ac levels (H4K20m3 was not changed), down-regulations of miR21 and miR155 in low-dose cinnamon groups (miR22 and miR34a were not modulated), and significant reduction of the methylation status of two out of five gene promoters-ATM and TIMP3 (PITX2, RASSF1, PTEN promoters were not changed). In vitro study confirmed results of animal studies, in that the essential oil of C. zeylanicum displayed significant anticancer efficacy in MCF-7 and MDA-MB-231 cells (using MTS, BrdU, cell cycle, annexin V/PI, caspase-3/7, Bcl-2, PARP, and mitochondrial membrane potential analyses). As a conclusion, C. zeylanicum L. showed chemopreventive and therapeutic activities in animal breast carcinoma models that were also significantly confirmed by mechanistic evaluations in vitro and in vivo.
Department of Immunology and School of Medicine Keimyung University Dalseo Gu 42601 Daegu Korea
Department of Medical Biology Faculty of Medicine P J Safarik University 04011 Kosice Slovakia
Department of Pharmacology Faculty of Medicine P J Šafarik University 040 11 Košice Slovakia
OBGY Health and Care Ltd 01001 Zilina Slovakia
Weill Cornell Medicine in Qatar Qatar Foundation Education City 24144 Doha Qatar
References provided by Crossref.org
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