• Je něco špatně v tomto záznamu ?

Comparative analysis of chromosome 2A molecular organization in diploid and hexaploid wheat

P. Kaur, S. Jindal, B. Yadav, I. Yadav, A. Mahato, P. Sharma, S. Kaur, OP. Gupta, J. Vrána, H. Šimková, J. Doležel, BS. Gill, KFX. Meyer, JP. Khurana, NK. Singh, P. Chhuneja, K. Singh,

. 2020 ; 47 (3) : 1991-2003. [pub] 20200208

Jazyk angličtina Země Nizozemsko

Typ dokumentu srovnávací studie, časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc20028459

Grantová podpora
BT/IWGSC/03/TF/2008 Department of Biotechnology, New Delhi
CZ.02.1.01/0.0/0.0/16_019/0000827 European Regional Development Fund

E-zdroje Online Plný text

NLK ProQuest Central od 1997-01-01 do Před 1 rokem
Medline Complete (EBSCOhost) od 2011-01-01 do Před 1 rokem
Health & Medicine (ProQuest) od 1997-01-01 do Před 1 rokem

Odkazy

PubMed 32034627
DOI 10.1007/s11033-020-05295-9
Knihovny.cz E-zdroje

Diploid A genome wheat species harbor immense genetic variability which has been targeted and proven useful in wheat improvement. Development and deployment of sequence-based markers has opened avenues for comparative analysis, gene transfer and marker assisted selection (MAS) using high throughput cost effective genotyping techniques. Chromosome 2A of wheat is known to harbor several economically important genes. The present study aimed at identification of genic sequences corresponding to full length cDNAs and mining of SSRs and ISBPs from 2A draft sequence assembly of hexaploid wheat cv. Chinese Spring for marker development. In total, 1029 primer pairs including 478 gene derived, 501 SSRs and 50 ISBPs were amplified in diploid A genome species Triticum monococcum and T. boeoticum identifying 221 polymorphic loci. Out of these, 119 markers were mapped onto a pre-existing chromosome 2A genetic map consisting of 42 mapped markers. The enriched genetic map constituted 161 mapped markers with final map length of 549.6 cM. Further, 2A genetic map of T. monococcum was anchored to the physical map of 2A of cv. Chinese Spring which revealed several rearrangements between the two species. The present study generated a highly saturated genetic map of 2A and physical anchoring of genetically mapped markers revealed a complex genetic architecture of chromosome 2A that needs to be investigated further.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc20028459
003      
CZ-PrNML
005      
20210114153931.0
007      
ta
008      
210105s2020 ne f 000 0|eng||
009      
AR
024    7_
$a 10.1007/s11033-020-05295-9 $2 doi
035    __
$a (PubMed)32034627
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Kaur, Parampreet $u School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141004, India. parampreet162906@pau.edu. School of Organic Farming, Punjab Agricultural University, Ludhiana, 141004, India. parampreet162906@pau.edu.
245    10
$a Comparative analysis of chromosome 2A molecular organization in diploid and hexaploid wheat / $c P. Kaur, S. Jindal, B. Yadav, I. Yadav, A. Mahato, P. Sharma, S. Kaur, OP. Gupta, J. Vrána, H. Šimková, J. Doležel, BS. Gill, KFX. Meyer, JP. Khurana, NK. Singh, P. Chhuneja, K. Singh,
520    9_
$a Diploid A genome wheat species harbor immense genetic variability which has been targeted and proven useful in wheat improvement. Development and deployment of sequence-based markers has opened avenues for comparative analysis, gene transfer and marker assisted selection (MAS) using high throughput cost effective genotyping techniques. Chromosome 2A of wheat is known to harbor several economically important genes. The present study aimed at identification of genic sequences corresponding to full length cDNAs and mining of SSRs and ISBPs from 2A draft sequence assembly of hexaploid wheat cv. Chinese Spring for marker development. In total, 1029 primer pairs including 478 gene derived, 501 SSRs and 50 ISBPs were amplified in diploid A genome species Triticum monococcum and T. boeoticum identifying 221 polymorphic loci. Out of these, 119 markers were mapped onto a pre-existing chromosome 2A genetic map consisting of 42 mapped markers. The enriched genetic map constituted 161 mapped markers with final map length of 549.6 cM. Further, 2A genetic map of T. monococcum was anchored to the physical map of 2A of cv. Chinese Spring which revealed several rearrangements between the two species. The present study generated a highly saturated genetic map of 2A and physical anchoring of genetically mapped markers revealed a complex genetic architecture of chromosome 2A that needs to be investigated further.
650    _2
$a mapování chromozomů $x metody $7 D002874
650    _2
$a chromozomy rostlin $x genetika $7 D032461
650    _2
$a diploidie $7 D004171
650    _2
$a vysoce účinné nukleotidové sekvenování $7 D059014
650    _2
$a mikrosatelitní repetice $7 D018895
650    _2
$a jednonukleotidový polymorfismus $7 D020641
650    _2
$a polyploidie $7 D011123
650    12
$a lokus kvantitativního znaku $7 D040641
650    _2
$a sekvenční analýza DNA $7 D017422
650    _2
$a pšenice $x genetika $7 D014908
655    _2
$a srovnávací studie $7 D003160
655    _2
$a časopisecké články $7 D016428
700    1_
$a Jindal, Suruchi $u School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141004, India.
700    1_
$a Yadav, Bharat $u School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141004, India.
700    1_
$a Yadav, Inderjit $u School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141004, India.
700    1_
$a Mahato, Ajay $u ICAR-National Institute for Plant Biotechnology, New Delhi, 110012, India.
700    1_
$a Sharma, Priti $u School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141004, India.
700    1_
$a Kaur, Satinder $u School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141004, India.
700    1_
$a Gupta, O P $u College of Agriculture, Punjab Agricultural University, Ludhiana, 141004, India.
700    1_
$a Vrána, Jan $u Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, CZ-78371, Olomouc, Czech Republic.
700    1_
$a Šimková, Hana $u Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, CZ-78371, Olomouc, Czech Republic.
700    1_
$a Doležel, Jaroslav $u Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, CZ-78371, Olomouc, Czech Republic.
700    1_
$a Gill, Bikram Singh $u Kansas State University, Manhattan, KS, 66506, USA.
700    1_
$a Meyer, Klaus F X $u MIPS/IBIS, Helmholtz- Zentrum München, Ingolstädter Landstrasse 1, 85764, Neuherberg, Germany.
700    1_
$a Khurana, J P $u University of Delhi, South Campus, New Delhi, 110021, India.
700    1_
$a Singh, N K $u ICAR-National Institute for Plant Biotechnology, New Delhi, 110012, India.
700    1_
$a Chhuneja, Parveen $u School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141004, India.
700    1_
$a Singh, Kuldeep $u School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141004, India. ICAR-National Bureau of Plant Genetic Resources, New Delhi, 110012, India.
773    0_
$w MED00003392 $t Molecular biology reports $x 1573-4978 $g Roč. 47, č. 3 (2020), s. 1991-2003
856    41
$u https://pubmed.ncbi.nlm.nih.gov/32034627 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20210105 $b ABA008
991    __
$a 20210114153928 $b ABA008
999    __
$a ok $b bmc $g 1608794 $s 1119639
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 47 $c 3 $d 1991-2003 $e 20200208 $i 1573-4978 $m Molecular biology reports $n Mol Biol Rep $x MED00003392
GRA    __
$a BT/IWGSC/03/TF/2008 $p Department of Biotechnology, New Delhi
GRA    __
$a CZ.02.1.01/0.0/0.0/16_019/0000827 $p European Regional Development Fund
LZP    __
$a Pubmed-20210105
Zpět

Najít záznam

Citační ukazatele

Možnosti archivace