Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

The juvenile hormone described in Rhodnius prolixus by Wigglesworth is juvenile hormone III skipped bisepoxide

MJ. Villalobos-Sambucaro, M. Nouzova, CE. Ramirez, M. Eugenia Alzugaray, F. Fernandez-Lima, JR. Ronderos, FG. Noriega,

. 2020 ; 10 (1) : 3091. [pub] 20200220

Language English Country Great Britain

Document type Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc20028490

Grant support
R21AI135469 NIH HHS - United States
R01AI045545 NIH HHS - United States

Juvenile hormones (JHs) are sesquiterpenoids synthesized by the corpora allata (CA). They play critical roles during insect development and reproduction. The first JH was described in 1934 as a "metamorphosis inhibitory hormone" in Rhodnius prolixus by Sir Vincent B. Wigglesworth. Remarkably, in spite of the importance of R. prolixus as vectors of Chagas disease and model organisms in insect physiology, the original JH that Wigglesworth described for the kissing-bug R. prolixus remained unidentified. We employed liquid chromatography mass spectrometry to search for the JH homologs present in the hemolymph of fourth instar nymphs of R. prolixus. Wigglesworth's original JH is the JH III skipped bisepoxide (JHSB3), a homolog identified in other heteropteran species. Changes in the titer of JHSB3 were studied during the 10-day long molting cycle of 4th instar nymph, between a blood meal and the ecdysis to 5th instar. In addition we measured the changes of mRNA levels in the CA for the 13 enzymes of the JH biosynthetic pathway during the molting cycle of 4th instar. Almost 90 years after the first descriptions of the role of JH in insects, this study finally reveals that the specific JH homolog responsible for Wigglesworth's original observations is JHSB3.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc20028490
003      
CZ-PrNML
005      
20210114154030.0
007      
ta
008      
210105s2020 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1038/s41598-020-59495-1 $2 doi
035    __
$a (PubMed)32080221
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Villalobos-Sambucaro, Maria Jose $u Cátedra Histología y Embriología Animal (FCNyM-UNLP), La Plata, Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET-ARGENTINA), Buenos Aires, Argentina.
245    14
$a The juvenile hormone described in Rhodnius prolixus by Wigglesworth is juvenile hormone III skipped bisepoxide / $c MJ. Villalobos-Sambucaro, M. Nouzova, CE. Ramirez, M. Eugenia Alzugaray, F. Fernandez-Lima, JR. Ronderos, FG. Noriega,
520    9_
$a Juvenile hormones (JHs) are sesquiterpenoids synthesized by the corpora allata (CA). They play critical roles during insect development and reproduction. The first JH was described in 1934 as a "metamorphosis inhibitory hormone" in Rhodnius prolixus by Sir Vincent B. Wigglesworth. Remarkably, in spite of the importance of R. prolixus as vectors of Chagas disease and model organisms in insect physiology, the original JH that Wigglesworth described for the kissing-bug R. prolixus remained unidentified. We employed liquid chromatography mass spectrometry to search for the JH homologs present in the hemolymph of fourth instar nymphs of R. prolixus. Wigglesworth's original JH is the JH III skipped bisepoxide (JHSB3), a homolog identified in other heteropteran species. Changes in the titer of JHSB3 were studied during the 10-day long molting cycle of 4th instar nymph, between a blood meal and the ecdysis to 5th instar. In addition we measured the changes of mRNA levels in the CA for the 13 enzymes of the JH biosynthetic pathway during the molting cycle of 4th instar. Almost 90 years after the first descriptions of the role of JH in insects, this study finally reveals that the specific JH homolog responsible for Wigglesworth's original observations is JHSB3.
650    _2
$a zvířata $7 D000818
650    _2
$a corpora allata $x chemie $7 D003335
650    _2
$a epoxidové sloučeniny $x chemie $7 D004852
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a hemolymfa $x chemie $7 D006458
650    12
$a biologická proměna $7 D008675
650    _2
$a shazování tělního pokryvu $x fyziologie $7 D018978
650    _2
$a nymfa $x chemie $x fyziologie $7 D009758
650    _2
$a kukla $x chemie $x fyziologie $7 D011679
650    _2
$a Rhodnius $x chemie $x fyziologie $7 D012239
650    _2
$a seskviterpeny $x chemie $7 D012717
655    _2
$a časopisecké články $7 D016428
655    _2
$a Research Support, N.I.H., Extramural $7 D052061
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Nouzova, Marcela $u Department of Biological Sciences and Biomolecular Science Institute, Florida International University, Miami, FL, USA. Institute of Parasitology, Biology Centre CAS, Ceske Budejovice, Czech Republic.
700    1_
$a Ramirez, Cesar E $u Department of Chemistry and Biochemistry and Biomolecular Science Institute, Florida International University, Miami, USA.
700    1_
$a Eugenia Alzugaray, María $u Cátedra Histología y Embriología Animal (FCNyM-UNLP), La Plata, Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET-ARGENTINA), Buenos Aires, Argentina.
700    1_
$a Fernandez-Lima, Francisco $u Department of Chemistry and Biochemistry and Biomolecular Science Institute, Florida International University, Miami, USA.
700    1_
$a Ronderos, Jorge Rafael $u Cátedra Histología y Embriología Animal (FCNyM-UNLP), La Plata, Argentina. jronderos@fcnym.unlp.edu.ar.
700    1_
$a Noriega, Fernando G $u Department of Biological Sciences and Biomolecular Science Institute, Florida International University, Miami, FL, USA. noriegaf@fiu.edu.
773    0_
$w MED00182195 $t Scientific reports $x 2045-2322 $g Roč. 10, č. 1 (2020), s. 3091
856    41
$u https://pubmed.ncbi.nlm.nih.gov/32080221 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20210105 $b ABA008
991    __
$a 20210114154027 $b ABA008
999    __
$a ok $b bmc $g 1608825 $s 1119670
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 10 $c 1 $d 3091 $e 20200220 $i 2045-2322 $m Scientific reports $n Sci Rep $x MED00182195
GRA    __
$a R21AI135469 $p NIH HHS $2 United States
GRA    __
$a R01AI045545 $p NIH HHS $2 United States
LZP    __
$a Pubmed-20210105

Find record

Citation metrics

Archiving options