• Je něco špatně v tomto záznamu ?

Methods to assess DC-dependent priming of T cell responses by dying cells

AM. Chalupova, S. Vosahlikova, D. Rozkova, K. Sochorova, L. Palova-Jelinkova, J. Bartunkova, R. Spisek, J. Fucikova,

. 2020 ; 632 (-) : 55-65. [pub] 20190703

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc20028649

Dendritic cells have been widely investigated in cancer immunotherapy clinical trials for the last two decades mainly due to their robust ability to elicit an adaptive anticancer immune response of the cellular and humoral types. Immature DCs can be easily loaded with desired antigens. However, to become efficient antigen-presenting cells, DCs must first undergo a process of maturation. Protocols for the generation of DCs for use in cancer immunotherapy, including the generation of a large number of immature DCs for antigen pulsing and the selection of a well-defined immunostimulatory agent to achieve complete and reproducible maturation, which is a crucial step for further stimulation of T cell activation, must carefully consider the characteristics of DC physiology. In this report, we provided a detailed protocol for DC generation, pulsation and activation with the subsequent induction of T cell-specific immune responses.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc20028649
003      
CZ-PrNML
005      
20210114154555.0
007      
ta
008      
210105s2020 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1016/bs.mie.2019.05.045 $2 doi
035    __
$a (PubMed)32000914
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Chalupova, Anne-Marie $u Sotio, Prague, Czech Republic.
245    10
$a Methods to assess DC-dependent priming of T cell responses by dying cells / $c AM. Chalupova, S. Vosahlikova, D. Rozkova, K. Sochorova, L. Palova-Jelinkova, J. Bartunkova, R. Spisek, J. Fucikova,
520    9_
$a Dendritic cells have been widely investigated in cancer immunotherapy clinical trials for the last two decades mainly due to their robust ability to elicit an adaptive anticancer immune response of the cellular and humoral types. Immature DCs can be easily loaded with desired antigens. However, to become efficient antigen-presenting cells, DCs must first undergo a process of maturation. Protocols for the generation of DCs for use in cancer immunotherapy, including the generation of a large number of immature DCs for antigen pulsing and the selection of a well-defined immunostimulatory agent to achieve complete and reproducible maturation, which is a crucial step for further stimulation of T cell activation, must carefully consider the characteristics of DC physiology. In this report, we provided a detailed protocol for DC generation, pulsation and activation with the subsequent induction of T cell-specific immune responses.
650    _2
$a zvířata $7 D000818
650    _2
$a apoptóza $7 D017209
650    _2
$a kultivované buňky $7 D002478
650    _2
$a dendritické buňky $x imunologie $7 D003713
650    _2
$a průtoková cytometrie $x metody $7 D005434
650    _2
$a lidé $7 D006801
650    12
$a aktivace lymfocytů $7 D008213
650    _2
$a nádory $x imunologie $7 D009369
650    _2
$a fagocytóza $7 D010587
650    _2
$a T-lymfocyty $x imunologie $7 D013601
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Vosahlikova, Sarka $u Sotio, Prague, Czech Republic.
700    1_
$a Rozkova, Daniela $u Sotio, Prague, Czech Republic.
700    1_
$a Sochorova, Klara $u Sotio, Prague, Czech Republic.
700    1_
$a Palova-Jelinkova, Lenka $u Department of Immunology, Charles University, 2(nd) Faculty of Medicine and University Hospital Motol, Prague, Czech Republic; Sotio, Prague, Czech Republic.
700    1_
$a Bartunkova, Jirina $u Department of Immunology, Charles University, 2(nd) Faculty of Medicine and University Hospital Motol, Prague, Czech Republic; Sotio, Prague, Czech Republic.
700    1_
$a Spisek, Radek $u Department of Immunology, Charles University, 2(nd) Faculty of Medicine and University Hospital Motol, Prague, Czech Republic; Sotio, Prague, Czech Republic.
700    1_
$a Fucikova, Jitka $u Department of Immunology, Charles University, 2(nd) Faculty of Medicine and University Hospital Motol, Prague, Czech Republic; Sotio, Prague, Czech Republic. Electronic address: fucikova@sotio.com.
773    0_
$w MED00008502 $t Methods in enzymology $x 1557-7988 $g Roč. 632, č. - (2020), s. 55-65
856    41
$u https://pubmed.ncbi.nlm.nih.gov/32000914 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20210105 $b ABA008
991    __
$a 20210114154551 $b ABA008
999    __
$a ok $b bmc $g 1608984 $s 1119829
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 632 $c - $d 55-65 $e 20190703 $i 1557-7988 $m Methods in enzymology $n Methods Enzymol $x MED00008502
LZP    __
$a Pubmed-20210105

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace