Glycosylation is a topic of intense current interest in the development of biopharmaceuticals because it is related to drug safety and efficacy. This work describes results of an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material. Seventy-six laboratories from industry, university, research, government, and hospital sectors in Europe, North America, Asia, and Australia submitted a total of 103 reports on glycan distributions. The principal objective of this study was to report and compare results for the full range of analytical methods presently used in the glycosylation analysis of mAbs. Therefore, participation was unrestricted, with laboratories choosing their own measurement techniques. Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification. Consequently, the diversity of results was enormous, with the number of glycan compositions identified by each laboratory ranging from 4 to 48. In total, one hundred sixteen glycan compositions were reported, of which 57 compositions could be assigned consensus abundance values. These consensus medians provide community-derived values for NISTmAb PS. Agreement with the consensus medians did not depend on the specific method or laboratory type. The study provides a view of the current state-of-the-art for biologic glycosylation measurement and suggests a clear need for harmonization of glycosylation analysis methods.

Advanced Therapy Products Research Division Korea National Institute of Food and Drug Safety 187 Osongsaengmyeong 2 ro Osong eup Heungdeok gu Cheongju si Chungcheongbuk do 363 700 Korea

Agilent Technologies Inc 5301 Stevens Creek Blvd Santa Clara California 95051

Alberta Glycomics Centre University of Alberta Edmonton Alberta T6G 2G2 Canada Department of Chemistry University of Alberta Edmonton Alberta T6G 2G2 Canada

Analytical Development Agensys Inc 1800 Steward Street Santa Monica California 90404

Analytical Development Biogen 14 Cambridge Center Cambridge Massachusetts 02142

Analytical R and D MilliporeSigma 2909 Laclede Ave St Louis Missouri 63103

Analytical Services Complex Carbohydrate Research Center University of Georgia 315 Riverbend Road Athens Georgia 30602

Astellas Pharma 5 2 3 Tokodai Tsukiba Ibaraki 300 2698 Japan

AstraZeneca Granta Park Cambridgeshire CB21 6GH United Kingdom

Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences Utrecht University Padualaan 8 3584 CH Utrecht The Netherlands

Biochemistry Method Development and Validation Eurofins Lancaster Laboratories Inc 2425 New Holland Pike Lancaster Pennsylvania 17601

BioCMC Solutions Covance Laboratories Limited Otley Road Harrogate North Yorks HG3 1PY United Kingdom

Biomolecular Discovery and Design Research Centre and ARC Centre of Excellence for Nanoscale BioPhotonics Macquarie University North Ryde Australia

Bioprocessing Technology Institute 20 Biopolis Way Level 3 Singapore 138668

Bruker Daltonik GmbH Fahrenheitstr 4 28359 Bremen Germany

Bruker Daltonik GmbH Fahrenheitstr 4 28359 Bremen Germany Department of Life Sciences and Technology Beuth Hochschule für Technik Berlin Seestraβe 64 13347 Berlin Germany

Center for Biologics Evaluation and Research Food and Drug Administration 10903 New Hampshire Avenue Silver Spring Maryland 20993

Center for Biomedical Mass Spectrometry Boston University School of Medicine 670 Albany Street Boston Massachusetts 02118

Center for Proteomics and Metabolomics Leiden University Medical Center P O Box 9600 2300 RC Leiden The Netherlands

Centre d'Immunologie Pierre Fabre 5 Avenue Napoléon 3 BP 60497 74164 St Julien en Genevois France

Chemical Sciences Division Material Measurement Laboratory National Institute of Standards and Technology 100 Bureau Drive Gaithersburg Maryland 20899

Children's GMP LLC St Jude Children's Research Hospital 262 Danny Thomas Place Memphis Tennessee 38105

CICbiomaGUNE Paseo Miramon 182 20009 San Sebastian Spain

Delaware Biotechnology Institute University of Delaware 15 Innovation Way Newark Delaware 19711

Department of Biomolecular Sciences Max Planck Institute of Colloids and Interfaces 14424 Potsdam Germany

Department of Chemistry and Biochemistry Texas Tech University 2500 Broadway Lubbock Texas 79409

Department of Chemistry Georgia State University 100 Piedmont Avenue Atlanta Georgia 30303

Department of Chemistry North Carolina State University 2620 Yarborough Drive Raleigh North Carolina 27695

Department of Chemistry University of Alberta Edmonton Alberta T6G 2G2 Canada

Department of Chemistry University of California One Shields Ave Davis California 95616

Department of Chemistry University of Hamburg Martin Luther King Pl 6 20146 Hamburg Germany

Department of Chemistry University of Manitoba 144 Dysart Road Winnipeg Manitoba Canada R3T 2N2

Department of Chemistry University of Manitoba 144 Dysart Road Winnipeg Manitoba Canada R3T 2N2 Agilent Technologies Inc 5301 Stevens Creek Blvd Santa Clara California 95051

Department of Chemistry University of Natural Resources and Life Science Vienna Muthgasse 18 1190 Wien Austria

Department of Chemistry Waters Corporation 34 Maple Street Milford Massachusetts 01757

Department of Clinical Chemistry and Transfusion Medicine Sahlgrenska Academy at the University of Gothenburg Bruna Straket 16 41345 Gothenburg Sweden

Department of Medical Biochemistry and Cell Biology University of Gothenburg Institute of Biomedicine Sahlgrenska Academy Medicinaregatan 9A Box 440 405 30 Gothenburg Sweden

Department of Medical Physiology Jagiellonian University Medical College ul Michalowskiego 12 31 126 Krakow Poland

Department of Pathology Johns Hopkins University 400 N Broadway Street Baltimore Maryland 21287

Department of Urology Boston Children's Hospital 300 Longwood Avenue Boston Massachusetts 02115

Division of Bioanalytical Chemistry Amsterdam Institute for Molecules Medicines and Systems Vrije Universiteit Amsterdam de Boelelaan 1085 1081 HV Amsterdam The Netherlands

Division of Biological Chemistry and Biologicals National Institute of Health Sciences 1 18 1 Kamiyoga Setagaya ku Tokyo 158 8501 Japan

Division of Mass Spectrometry Korea Basic Science Institute 162 YeonGuDanji Ro Ochang eup Cheongwon gu Cheongju Chungbuk 363 883 Korea

Exploratory Research Center on Life and Living Systems National Institutes of Natural Sciences 5 1 Higashiyama Myodaiji Okazaki 444 8787 Japan Graduate School of Pharmaceutical Sciences Nagoya City University 3 1 Tanabe dori Mizuhoku Nagoya 467 8603 Japan

GlycoScience Group The National Institute for Bioprocessing Research and Training Fosters Avenue Mount Merrion Blackrock Co Dublin Ireland

Glycoscience Research Laboratory Genos Borongajska cesta 83h 10 000 Zagreb Croatia

Glycoscience Research Laboratory Genos Borongajska cesta 83h 10 000 Zagreb Croatia Faculty of Pharmacy and Biochemistry University of Zagreb A Kovačića 1 10 000 Zagreb Croatia

glyXera GmbH Brenneckestrasse 20 * ZENIT 39120 Magdeburg Germany

glyXera GmbH Brenneckestrasse 20 * ZENIT 39120 Magdeburg Germany AstraZeneca Granta Park Cambridgeshire CB21 6GH United Kingdom

Graduate School of Advanced Sciences of Matter Hiroshima University 1 3 1 Kagamiyama Higashi Hiroshima 739 8530 Japan

Graduate School of Analytical Science and Technology Chungnam National University Gung dong 220 Yuseong Gu Daejeon 305 764 Korea

Graduate School of Pharmaceutical Sciences Nagoya City University 3 1 Tanabe dori Mizuhoku Nagoya 467 8603 Japan

Graduate School of Pharmaceutical Sciences Nagoya City University 3 1 Tanabe dori Mizuhoku Nagoya 467 8603 Japan Medical and Biological Laboratories Co Ltd 2 22 8 Chikusa Chikusa ku Nagoya 464 0858 Japan

Health Products and Foods Branch Health Canada AL 2201E 251 Sir Frederick Banting Driveway Ottawa Ontario K1A 0K9 Canada

Horváth Csaba Memorial Laboratory for Bioseparation Sciences Research Center for Molecular Medicine Doctoral School of Molecular Medicine Faculty of Medicine University of Debrecen Debrecen Egyetem ter 1 Hungary

Horváth Csaba Memorial Laboratory for Bioseparation Sciences Research Center for Molecular Medicine Doctoral School of Molecular Medicine Faculty of Medicine University of Debrecen Debrecen Egyetem ter 1 Hungary Translational Glycomics Research Group Research Institute of Biomolecular and Chemical Engineering University of Pannonia Veszprem Egyetem ut 10 Hungary

ImmunoGen 830 Winter Street Waltham Massachusetts 02451

Institute of Biophysics Chinese Academy of Sciences 15 Da Tun Road Chaoyang District Beijing 100101 China

Key Lab of Intelligent Information Processing Institute of Computing Technology Chinese Academy of Sciences 15 Da Tun Road Chaoyang District Beijing 100101 China

Koichi Tanaka Mass Spectrometry Research Laboratory Shimadzu Corporation 1 Nishinokyo Kuwabara cho Nakagyo ku Kyoto 604 8511 Japan

Laboratory of Mass Spectrometry of Interactions and Systems University of Strasbourg UMR Unistra CNRS 7140 France

Ludger Limited Culham Science Centre Abingdon Oxfordshire OX14 3EB United Kingdom

Mass Spec Core Facility KBI Biopharma 1101 Hamlin Road Durham North Carolina 27704

Mass Spectrometry Data Center Biomolecular Measurement Division Material Measurement Laboratory National Institute of Standards and Technology 100 Bureau Drive Gaithersburg Maryland 20899

Max Planck Institute for Dynamics of Complex Technical Systems Sandtorstrasse 1 39106 Magdeburg Germany

Merck 2015 Galloping Hill Rd Kenilworth New Jersey 07033

MS Bioworks LLC 3950 Varsity Drive Ann Arbor Michigan 48108

MSD Molenstraat 110 5342 CC Oss The Netherlands

National Institute for Biological Standards and Control Blanche Lane South Mimms Potters Bar Hertfordshire EN6 3QG United Kingdom

Natural and Medical Sciences Institute University of Tübingen Markwiesenstraβe 55 72770 Reutlingen Germany

New England Biolabs Inc 240 County Road Ipswich Massachusetts 01938

New York University 100 Washington Square East New York City New York 10003

Pantheon 201 College Road East Princeton New Jersey 08540

Pfizer Inc 1 Burtt Road Andover Massachusetts 01810

Proteodynamics ZI La Varenne 20 22 rue Henri et Gilberte Goudier 63200 RIOM France

Proteomics Central European Institute for Technology Masaryk University Kamenice 5 A26 625 00 BRNO Czech Republic

Proteomics Core Facility University of Gothenburg Medicinaregatan 1G SE 41390 Gothenburg Sweden

ProZyme Inc 3832 Bay Center Place Hayward California 94545

Sumitomo Bakelite Co Ltd 1 5 Muromati 1 Chome Nishiku Kobe 651 2241 Japan

Synthon Biopharmaceuticals Microweg 22 P O Box 7071 6503 GN Nijmegen The Netherlands

Takeda Pharmaceuticals International Co 40 Landsdowne Street Cambridge Massachusetts 02139

Target Discovery Institute Nuffield Department of Medicine University of Oxford Roosevelt Drive Oxford OX3 7FZ United Kingdom

Thermo Fisher Scientific 1214 Oakmead Parkway Sunnyvale California 94085

United States Pharmacopeia India Pvt Ltd IKP Knowledge Park Genome Valley Shamirpet Turkapally Village Medchal District Hyderabad 500 101 Telangana India

Zoetis 333 Portage St Kalamazoo Michigan 49007

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520    9_

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$a Duewer, David L $u Chemical Sciences Division, Material Measurement Laboratory, National Institute of Standards and Technology, 100 Bureau Drive Gaithersburg, Maryland 20899.

700    1_

$a Fung, Adam $u Analytical Development, Agensys, Inc., 1800 Steward Street Santa Monica, California 90404.

700    1_

$a Liu, Lily $u Analytical Development, Agensys, Inc., 1800 Steward Street Santa Monica, California 90404.

700    1_

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700    1_

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700    1_

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700    1_

$a Furuki, Kenichiro $u Astellas Pharma, 5-2-3 Tokodai, Tsukiba, Ibaraki, 300-2698, Japan.

700    1_

$a Frenkel, Ruth $u Analytical Development, Biogen, 14 Cambridge Center Cambridge, Massachusetts 02142.

700    1_

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700    1_

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700    1_

$a Zhang, Peiqing $u Bioprocessing Technology Institute, 20 Biopolis Way, Level 3 Singapore 138668.

700    1_

$a Altmann, Friedrich $u Department of Chemistry, University of Natural Resources and Life Science, Vienna (BOKU), Muthgasse 18 1190 Wien, Austria.

700    1_

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700    1_

$a Shao, Chun $u Center for Biomedical Mass Spectrometry, Boston University School of Medicine, 670 Albany Street Boston, Massachusetts 02118.

700    1_

$a Zaia, Joseph $u Center for Biomedical Mass Spectrometry, Boston University School of Medicine, 670 Albany Street Boston, Massachusetts 02118.

700    1_

$a Evers, Waltraud $u Bruker Daltonik GmbH, Fahrenheitstr. 4, 28359 Bremen, Germany.

700    1_

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700    1_

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700    1_

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700    1_

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700    1_

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700    1_

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700    1_

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700    1_

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700    1_

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700    1_

$a Liu, Yaming $u Institute of Biophysics, Chinese Academy of Sciences, 15 Da Tun Road, Chaoyang District, Beijing 100101 China.

700    1_

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700    1_

$a Wang, Yaojun $u Key Lab of Intelligent Information Processing, Institute of Computing Technology, Chinese Academy of Sciences, 15 Da Tun Road, Chaoyang District, Beijing 100101 China.

700    1_

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700    1_

$a An, Hyun Joo $u Graduate School of Analytical Science and Technology, Chungnam National University, Gung-dong 220, Yuseong-Gu, Daejeon 305-764, Korea (South).

700    1_

$a Reichardt, Niels-Christian $u CICbiomaGUNE, Paseo Miramon 182, 20009 San Sebastian, Spain.

700    1_

$a Ruiz, Juan Echevarria $u CICbiomaGUNE, Paseo Miramon 182, 20009 San Sebastian, Spain.

700    1_

$a Archer-Hartmann, Stephanie $u Analytical Services, Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road Athens, Georgia 30602.

700    1_

$a Azadi, Parastoo $u Analytical Services, Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road Athens, Georgia 30602.

700    1_

$a Bell, Len $u BioCMC Solutions (Large Molecules), Covance Laboratories Limited, Otley Road, Harrogate, North Yorks HG3 1PY, United Kingdom.

700    1_

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700    1_

$a An, Yanming $u Center for Biologics Evaluation and Research, Food and Drug Administration, 10903 New Hampshire Avenue, Silver Spring, Maryland 20993.

700    1_

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700    1_

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700    1_

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700    1_

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700    1_

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700    1_

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700    1_

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700    1_

$a Cyr, Terry D $u Health Products and Foods Branch, Health Canada, AL 2201E, 251 Sir Frederick Banting Driveway, Ottawa, Ontario, K1A 0K9 Canada.

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700    1_

$a Sugiyama, Taiki $u Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama Higashi-Hiroshima 739-8530 Japan.

700    1_

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700    1_

$a Jaworek, Jolanta $u Department of Medical Physiology, Jagiellonian University Medical College, ul. Michalowskiego 12, 31-126 Krakow, Poland.

700    1_

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700    1_

$a Zhang, Hui $u Department of Pathology, Johns Hopkins University, 400 N. Broadway Street Baltimore, Maryland 21287.

700    1_

$a Kelly, Tim $u Mass Spec Core Facility, KBI Biopharma, 1101 Hamlin Road Durham, North Carolina 27704.

700    1_

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700    1_

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700    1_

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700    1_

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700    1_

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700    1_

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$a Lindo, Viv $u AstraZeneca, Granta Park, Cambridgeshire, CB21 6GH United Kingdom.

700    1_

$a Chen, Yuetian $u Merck, 2015 Galloping Hill Rd, Kenilworth, New Jersey 07033.

700    1_

$a Goswami, Kudrat $u Merck, 2015 Galloping Hill Rd, Kenilworth, New Jersey 07033.

700    1_

$a Gau, Brian $u Analytical R&D, MilliporeSigma, 2909 Laclede Ave. St. Louis, Missouri 63103.

700    1_

$a Amunugama, Ravi $u MS Bioworks, LLC, 3950 Varsity Drive Ann Arbor, Michigan 48108.

700    1_

$a Jones, Richard $u MS Bioworks, LLC, 3950 Varsity Drive Ann Arbor, Michigan 48108.

700    1_

$a Stroop, Corné J M $u MSD, Molenstraat 110, 5342 CC Oss, The Netherlands.

700    1_

$a Kato, Koichi $u Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji, Okazaki 444-8787 Japan. Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuhoku, Nagoya 467-8603 Japan.

700    1_

$a Yagi, Hirokazu $u Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuhoku, Nagoya 467-8603 Japan.

700    1_

$a Kondo, Sachiko $u Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuhoku, Nagoya 467-8603 Japan. Medical & Biological Laboratories Co., Ltd, 2-22-8 Chikusa, Chikusa-ku, Nagoya 464-0858 Japan.

700    1_

$a Yuen, C T $u National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG United Kingdom.

700    1_

$a Harazono, Akira $u Division of Biological Chemistry & Biologicals, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501 Japan.

700    1_

$a Shi, Xiaofeng $u New England Biolabs, Inc., 240 County Road, Ipswich, Massachusetts 01938.

700    1_

$a Magnelli, Paula E $u New England Biolabs, Inc., 240 County Road, Ipswich, Massachusetts 01938.

700    1_

$a Kasper, Brian T $u New York University, 100 Washington Square East New York City, New York 10003.

700    1_

$a Mahal, Lara $u New York University, 100 Washington Square East New York City, New York 10003.

700    1_

$a Harvey, David J $u Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Roosevelt Drive, Oxford, OX3 7FZ, United Kingdom.

700    1_

$a O'Flaherty, Roisin $u GlycoScience Group, The National Institute for Bioprocessing Research and Training, Fosters Avenue, Mount Merrion, Blackrock, Co. Dublin, Ireland.

700    1_

$a Rudd, Pauline M $u GlycoScience Group, The National Institute for Bioprocessing Research and Training, Fosters Avenue, Mount Merrion, Blackrock, Co. Dublin, Ireland.

700    1_

$a Saldova, Radka $u GlycoScience Group, The National Institute for Bioprocessing Research and Training, Fosters Avenue, Mount Merrion, Blackrock, Co. Dublin, Ireland.

700    1_

$a Hecht, Elizabeth S $u Department of Chemistry, North Carolina State University, 2620 Yarborough Drive Raleigh, North Carolina 27695.

700    1_

$a Muddiman, David C $u Department of Chemistry, North Carolina State University, 2620 Yarborough Drive Raleigh, North Carolina 27695.

700    1_

$a Kang, Jichao $u Pantheon, 201 College Road East Princeton, New Jersey 08540.

700    1_

$a Bhoskar, Prachi $u Pfizer Inc., 1 Burtt Road Andover, Massachusetts 01810.

700    1_

$a Menard, Daniele $u Pfizer Inc., 1 Burtt Road Andover, Massachusetts 01810.

700    1_

$a Saati, Andrew $u Pfizer Inc., 1 Burtt Road Andover, Massachusetts 01810.

700    1_

$a Merle, Christine $u Proteodynamics, ZI La Varenne 20-22 rue Henri et Gilberte Goudier 63200 RIOM, France.

700    1_

$a Mast, Steven $u ProZyme, Inc., 3832 Bay Center Place Hayward, California 94545.

700    1_

$a Tep, Sam $u ProZyme, Inc., 3832 Bay Center Place Hayward, California 94545.

700    1_

$a Truong, Jennie $u ProZyme, Inc., 3832 Bay Center Place Hayward, California 94545.

700    1_

$a Nishikaze, Takashi $u Koichi Tanaka Mass Spectrometry Research Laboratory, Shimadzu Corporation, 1 Nishinokyo Kuwabara-cho Nakagyo-ku, Kyoto, 604 8511 Japan.

700    1_

$a Sekiya, Sadanori $u Koichi Tanaka Mass Spectrometry Research Laboratory, Shimadzu Corporation, 1 Nishinokyo Kuwabara-cho Nakagyo-ku, Kyoto, 604 8511 Japan.

700    1_

$a Shafer, Aaron $u Children's GMP LLC, St. Jude Children's Research Hospital, 262 Danny Thomas Place Memphis, Tennessee 38105.

700    1_

$a Funaoka, Sohei $u Sumitomo Bakelite Co., Ltd., 1-5 Muromati 1-Chome, Nishiku, Kobe, 651-2241 Japan.

700    1_

$a Toyoda, Masaaki $u Sumitomo Bakelite Co., Ltd., 1-5 Muromati 1-Chome, Nishiku, Kobe, 651-2241 Japan.

700    1_

$a de Vreugd, Peter $u Synthon Biopharmaceuticals, Microweg 22 P.O. Box 7071, 6503 GN Nijmegen, The Netherlands.

700    1_

$a Caron, Cassie $u Takeda Pharmaceuticals International Co., 40 Landsdowne Street Cambridge, Massachusetts 02139.

700    1_

$a Pradhan, Pralima $u Takeda Pharmaceuticals International Co., 40 Landsdowne Street Cambridge, Massachusetts 02139.

700    1_

$a Tan, Niclas Chiang $u Takeda Pharmaceuticals International Co., 40 Landsdowne Street Cambridge, Massachusetts 02139.

700    1_

$a Mechref, Yehia $u Department of Chemistry and Biochemistry, Texas Tech University, 2500 Broadway, Lubbock, Texas 79409.

700    1_

$a Patil, Sachin $u Thermo Fisher Scientific, 1214 Oakmead Parkway Sunnyvale, California 94085.

700    1_

$a Rohrer, Jeffrey S $u Thermo Fisher Scientific, 1214 Oakmead Parkway Sunnyvale, California 94085.

700    1_

$a Chakrabarti, Ranjan $u United States Pharmacopeia India Pvt. Ltd. IKP Knowledge Park, Genome Valley, Shamirpet, Turkapally Village, Medchal District, Hyderabad 500 101 Telangana, India.

700    1_

$a Dadke, Disha $u United States Pharmacopeia India Pvt. Ltd. IKP Knowledge Park, Genome Valley, Shamirpet, Turkapally Village, Medchal District, Hyderabad 500 101 Telangana, India.

700    1_

$a Lahori, Mohammedazam $u United States Pharmacopeia India Pvt. Ltd. IKP Knowledge Park, Genome Valley, Shamirpet, Turkapally Village, Medchal District, Hyderabad 500 101 Telangana, India.

700    1_

$a Zou, Chunxia $u Alberta Glycomics Centre, University of Alberta, Edmonton, Alberta T6G 2G2 Canada. Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2 Canada.

700    1_

$a Cairo, Christopher $u Alberta Glycomics Centre, University of Alberta, Edmonton, Alberta T6G 2G2 Canada. Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2 Canada.

700    1_

$a Reiz, Béla $u Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2 Canada.

700    1_

$a Whittal, Randy M $u Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2 Canada.

700    1_

$a Lebrilla, Carlito B $u Department of Chemistry, University of California, One Shields Ave, Davis, California 95616.

700    1_

$a Wu, Lauren $u Department of Chemistry, University of California, One Shields Ave, Davis, California 95616.

700    1_

$a Guttman, Andras $u Horváth Csaba Memorial Laboratory for Bioseparation Sciences, Research Center for Molecular Medicine, Doctoral School of Molecular Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Egyetem ter 1, Hungary.

700    1_

$a Szigeti, Marton $u Horváth Csaba Memorial Laboratory for Bioseparation Sciences, Research Center for Molecular Medicine, Doctoral School of Molecular Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Egyetem ter 1, Hungary. Translational Glycomics Research Group, Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, Veszprem, Egyetem ut 10, Hungary.

700    1_

$a Kremkow, Benjamin G $u Delaware Biotechnology Institute, University of Delaware, 15 Innovation Way Newark, Delaware 19711.

700    1_

$a Lee, Kelvin H $u Delaware Biotechnology Institute, University of Delaware, 15 Innovation Way Newark, Delaware 19711.

700    1_

$a Sihlbom, Carina $u Proteomics Core Facility, University of Gothenburg, Medicinaregatan 1G SE 41390 Gothenburg, Sweden.

700    1_

$a Adamczyk, Barbara $u Department of Medical Biochemistry and Cell Biology, University of Gothenburg, Institute of Biomedicine, Sahlgrenska Academy, Medicinaregatan 9A, Box 440, 405 30, Gothenburg, Sweden.

700    1_

$a Jin, Chunsheng $u Department of Medical Biochemistry and Cell Biology, University of Gothenburg, Institute of Biomedicine, Sahlgrenska Academy, Medicinaregatan 9A, Box 440, 405 30, Gothenburg, Sweden.

700    1_

$a Karlsson, Niclas G $u Department of Medical Biochemistry and Cell Biology, University of Gothenburg, Institute of Biomedicine, Sahlgrenska Academy, Medicinaregatan 9A, Box 440, 405 30, Gothenburg, Sweden.

700    1_

$a Örnros, Jessica $u Department of Medical Biochemistry and Cell Biology, University of Gothenburg, Institute of Biomedicine, Sahlgrenska Academy, Medicinaregatan 9A, Box 440, 405 30, Gothenburg, Sweden.

700    1_

$a Larson, Göran $u Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska Academy at the University of Gothenburg, Bruna Straket 16, 41345 Gothenburg, Sweden.

700    1_

$a Nilsson, Jonas $u Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska Academy at the University of Gothenburg, Bruna Straket 16, 41345 Gothenburg, Sweden.

700    1_

$a Meyer, Bernd $u Department of Chemistry, University of Hamburg, Martin Luther King Pl. 6 20146 Hamburg, Germany.

700    1_

$a Wiegandt, Alena $u Department of Chemistry, University of Hamburg, Martin Luther King Pl. 6 20146 Hamburg, Germany.

700    1_

$a Komatsu, Emy $u Department of Chemistry, University of Manitoba, 144 Dysart Road, Winnipeg, Manitoba, Canada R3T 2N2.

700    1_

$a Perreault, Helene $u Department of Chemistry, University of Manitoba, 144 Dysart Road, Winnipeg, Manitoba, Canada R3T 2N2.

700    1_

$a Bodnar, Edward D $u Department of Chemistry, University of Manitoba, 144 Dysart Road, Winnipeg, Manitoba, Canada R3T 2N2. Agilent Technologies, Inc., 5301 Stevens Creek Blvd Santa Clara, California 95051.

700    1_

$a Said, Nassur $u Laboratory of Mass Spectrometry of Interactions and Systems, University of Strasbourg, UMR Unistra-CNRS 7140, France.

700    1_

$a Francois, Yannis-Nicolas $u Laboratory of Mass Spectrometry of Interactions and Systems, University of Strasbourg, UMR Unistra-CNRS 7140, France.

700    1_

$a Leize-Wagner, Emmanuelle $u Laboratory of Mass Spectrometry of Interactions and Systems, University of Strasbourg, UMR Unistra-CNRS 7140, France.

700    1_

$a Maier, Sandra $u Natural and Medical Sciences Institute, University of Tübingen, Markwiesenstraβe 55, 72770 Reutlingen, Germany.

700    1_

$a Zeck, Anne $u Natural and Medical Sciences Institute, University of Tübingen, Markwiesenstraβe 55, 72770 Reutlingen, Germany.

700    1_

$a Heck, Albert J R $u Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.

700    1_

$a Yang, Yang $u Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.

700    1_

$a Haselberg, Rob $u Division of Bioanalytical Chemistry, Amsterdam Institute for Molecules, Medicines and Systems, Vrije Universiteit Amsterdam, de Boelelaan 1085, 1081 HV Amsterdam, The Netherlands.

700    1_

$a Yu, Ying Qing $u Department of Chemistry, Waters Corporation, 34 Maple Street Milford, Massachusetts 01757.

700    1_

$a Alley, William $u Department of Chemistry, Waters Corporation, 34 Maple Street Milford, Massachusetts 01757.

700    1_

$a Leone, Joseph W $u Zoetis, 333 Portage St. Kalamazoo, Michigan 49007.

700    1_

$a Yuan, Hua $u Zoetis, 333 Portage St. Kalamazoo, Michigan 49007.

700    1_

$a Stein, Stephen E $u Mass Spectrometry Data Center, Biomolecular Measurement Division, Material Measurement Laboratory, National Institute of Standards and Technology, 100 Bureau Drive Gaithersburg, Maryland 20899.

773    0_

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856    41

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