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Lytic and genomic properties of spontaneous host-range Kayvirus mutants prove their suitability for upgrading phage therapeutics against staphylococci
T. Botka, R. Pantůček, I. Mašlaňová, M. Benešík, P. Petráš, V. Růžičková, P. Havlíčková, M. Varga, H. Žemličková, I. Koláčková, M. Florianová, V. Jakubů, R. Karpíšková, J. Doškař,
Jazyk angličtina Země Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
NV16-29916A
MZ0
CEP - Centrální evidence projektů
Digitální knihovna NLK
Plný text - Článek
Free Medical Journals od 2011
Nature Open Access od 2011-12-01
PubMed Central od 2011
Europe PubMed Central od 2011
ProQuest Central od 2011-01-01
Open Access Digital Library od 2011-01-01
Open Access Digital Library od 2011-01-01
Health & Medicine (ProQuest) od 2011-01-01
ROAD: Directory of Open Access Scholarly Resources od 2011
Odkazy
PubMed
30940900
DOI
10.1038/s41598-019-41868-w
Knihovny.cz E-zdroje
- MeSH
- bakteriální léková rezistence MeSH
- bakteriofágy genetika MeSH
- délka genomu MeSH
- genom virový MeSH
- genomika MeSH
- jednonukleotidový polymorfismus MeSH
- methicilin farmakologie MeSH
- mutace * MeSH
- Staphylococcus aureus růst a vývoj virologie MeSH
- zastoupení bazí MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Lytic bacteriophages are valuable therapeutic agents against bacterial infections. There is continual effort to obtain new phages to increase the effectivity of phage preparations against emerging phage-resistant strains. Here we described the genomic diversity of spontaneous host-range mutants of kayvirus 812. Five mutant phages were isolated as rare plaques on phage-resistant Staphylococcus aureus strains. The host range of phage 812-derived mutants was 42% higher than the wild type, determined on a set of 186 methicillin-resistant S. aureus strains representing the globally circulating human and livestock-associated clones. Comparative genomics revealed that single-nucleotide polymorphisms from the parental phage 812 population were fixed in next-step mutants, mostly in genes for tail and baseplate components, and the acquired point mutations led to diverse receptor binding proteins in the phage mutants. Numerous genome changes associated with rearrangements between direct repeat motifs or intron loss were found. Alterations occurred in host-takeover and terminal genomic regions or the endolysin gene of mutants that exhibited the highest lytic activity, which implied various mechanisms of overcoming bacterial resistance. The genomic data revealed that Kayvirus spontaneous mutants are free from undesirable genes and their lytic properties proved their suitability for rapidly updating phage therapeutics.
Department of Experimental Biology Faculty of Science Masaryk University Brno 611 37 Czech Republic
National Institute of Public Health Praha 100 42 Czech Republic
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