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Integrative Approach to Phlebotomus mascittii Grassi, 1908: First Record in Vienna with New Morphological and Molecular Insights

E. Kniha, V. Dvořák, P. Halada, M. Milchram, AG. Obwaller, K. Kuhls, S. Schlegel, M. Köhsler, W. Poeppl, K. Bakran-Lebl, HP. Fuehrer, V. Volfová, G. Mooseder, V. Ivovic, P. Volf, J. Walochnik,

. 2020 ; 9 (12) : . [pub] 20201209

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc21001578

Grantová podpora
DOC fellowship Austrian Academy of Sciences
CZ02/2020 Austrian Agency for International Cooperation (ÖAW)
FA623A0507 Austrian Ministry of Defence
BIOCEV CZ1.05/1.1.00/02.0109 European Regional Development Fund
CePaViP European Regional Development Fund
FKZ01DK14021 Bundesministerium für Bildung und Forschung
Infravec No. 731060 Horizon 2020

Sand flies (Diptera: Psychodidae: Phlebotominae) are blood-feeding insects that transmit the protozoan parasites Leishmania spp. and various arthropod-borne (arbo) viruses. While in Mediterranean parts of Europe the sand fly fauna is diverse, in Central European countries including Austria mainly Phlebotomus mascittii is found, an assumed but unproven vector of Leishmania infantum. To update the currently understudied sand fly distribution in Austria, a sand fly survey was performed and other entomological catches were screened for sand flies. Seven new trapping locations of Ph. mascittii are reported including the first record in Vienna, representing also one of the first findings of this species in a city. Morphological identification, supported by fluorescence microscopy, was confirmed by two molecular approaches, including sequencing and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) protein profiling. Sand fly occurrence and activity were evaluated based on surveyed locations, habitat requirements and climatic parameters. Moreover, a first comparison of European Ph. mascittii populations was made by two marker genes, cytochrome c oxidase subunit 1 (COI), and cytochrome b (cytb), as well as MALDI-TOF mass spectra. Our study provides new important records of Ph. mascittii in Austria and valuable data for prospective entomological surveys. MALDI-TOF MS protein profiling was shown to be a reliable tool for differentiation between sand fly species. Rising temperatures and globalization demand for regular entomological surveys to monitor changes in species distribution and composition. This is also important with respect to the possible vector competence of Ph. mascittii.

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$a Sand flies (Diptera: Psychodidae: Phlebotominae) are blood-feeding insects that transmit the protozoan parasites Leishmania spp. and various arthropod-borne (arbo) viruses. While in Mediterranean parts of Europe the sand fly fauna is diverse, in Central European countries including Austria mainly Phlebotomus mascittii is found, an assumed but unproven vector of Leishmania infantum. To update the currently understudied sand fly distribution in Austria, a sand fly survey was performed and other entomological catches were screened for sand flies. Seven new trapping locations of Ph. mascittii are reported including the first record in Vienna, representing also one of the first findings of this species in a city. Morphological identification, supported by fluorescence microscopy, was confirmed by two molecular approaches, including sequencing and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) protein profiling. Sand fly occurrence and activity were evaluated based on surveyed locations, habitat requirements and climatic parameters. Moreover, a first comparison of European Ph. mascittii populations was made by two marker genes, cytochrome c oxidase subunit 1 (COI), and cytochrome b (cytb), as well as MALDI-TOF mass spectra. Our study provides new important records of Ph. mascittii in Austria and valuable data for prospective entomological surveys. MALDI-TOF MS protein profiling was shown to be a reliable tool for differentiation between sand fly species. Rising temperatures and globalization demand for regular entomological surveys to monitor changes in species distribution and composition. This is also important with respect to the possible vector competence of Ph. mascittii.
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$a Dvořák, Vít $u Department of Parasitology, Faculty of Science, Charles University Prague, 128 43 Prague, Czech Republic.
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$a Halada, Petr $u BioCeV, Institute of Microbiology of the Czech Academy of Sciences, 252 50 Vestec, Czech Republic.
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$a Milchram, Markus $u Department of Integrative Biology and Biodiversity Research, Institute of Zoology, University of Natural Resources and Life Sciences Vienna, 1180 Vienna, Austria.
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$a Obwaller, Adelheid G $u Federal Ministry of Defence, Division of Science, Research and Development, 1090 Vienna, Austria.
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$a Kuhls, Katrin $u Division Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, 15745 Wildau, Germany. Research Platform "Models & Simulation", Leibniz Centre for Agricultural Landscape Research (ZALF), 15374 Müncheberg, Germany.
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$a Schlegel, Susanne $u Division Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, 15745 Wildau, Germany. Division Microsystems Engineering, Technical University of Applied Sciences Wildau, 15745 Wildau, Germany.
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$a Köhsler, Martina $u Institute of Specific Prophylaxis and Tropical Medicine, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, Austria.
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$a Poeppl, Wolfgang $u Department of Dermatology and Tropical Medicine, Military Medical Cluster East, Austrian Armed Forces, 1210 Vienna, Austria.
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$a Bakran-Lebl, Karin $u Department of Pathobiology, Institute of Parasitology, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.
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$a Fuehrer, Hans-Peter $u Department of Pathobiology, Institute of Parasitology, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.
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$a Volfová, Věra $u Department of Parasitology, Faculty of Science, Charles University Prague, 128 43 Prague, Czech Republic.
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$a Mooseder, Gerhard $u Department of Dermatology and Tropical Medicine, Military Medical Cluster East, Austrian Armed Forces, 1210 Vienna, Austria.
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$a Ivovic, Vladimir $u Department of Biodiversity, FAMNIT, University of Primorska, 6000 Koper-Capodistria, Slovenia.
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$a Volf, Petr $u Department of Parasitology, Faculty of Science, Charles University Prague, 128 43 Prague, Czech Republic.
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$a Walochnik, Julia $u Institute of Specific Prophylaxis and Tropical Medicine, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, Austria.
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