How the acetate and propionate accumulation impact anaerobic syntrophy during methane formation is not well understood. To investigate such effect, continuous acetate (35 g/L), propionate (11.25 g/L) and bicarbonate (30 g/L) supplementation were used during mesophilic anaerobic digestion. The high throughput sequencing (16S rRNA and mcrA), Real-Time quantitative PCR, and stable carbon isotope fingerprinting were applied to investigate the structure and activity of microbial community members. The results demonstrated that the abundance of syntrophic acetate oxidizing bacteria exhibited a gradual decrease coupled with heavier stable carbon isotopic signature of methane (δ 13CH4) in the three reagents impacted reactors. The increased acetate and propionate concentrations exerted negative influence on biogas production but the relatively stable hydrogenotrophic methanogens together with syntrophic acetate/propionate oxidizing bacteria kept the stable methane formation facing acetate and propionate accumulation. The functional genes copy number of the hydrogenotrophic Methanocellaceae and Methanomicrobiaceae correlated significantly with δ 13CH4 (R2 > 0.74), but only the abundance of Methanocellaceae fitted well with δ 13CH4 (p < 0.05). The δ 13CH4 signatures can predict methanogenesis, as it directly reflects the main methanogenic pathway; yet, further investigation of isotope fractionation in acetate/propionate coupled with δ 13CH4 is needed. Collectively, these results provide deep insight into anaerobic syntrophy and reveal changes of synergistic relationships, both of which may contribute to the stability of biogas reactors.

Bioenergy Research Institute College of Biotechnology and Pharmaceutical Engineering Nanjing Tech University Nanjing 211816 China

Department of Applied Ecology Faculty of Environmental Sciences Czech University of Life Sciences Prague Kamýcká 129 16500 Prague Czech Republic

Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences Beijing 100081 China

School of Life Jiangsu Normal University Shanghai Road 101 221116 Xuzhou China

000      

00000naa a2200000 a 4500

001      

bmc21011417

003      

CZ-PrNML

005      

20210507105121.0

007      

ta

008      

210420s2021 xxu f 000 0|eng||

009      

AR

024    7_

$a 10.1016/j.wasman.2021.01.038 $2 doi

035    __

$a (PubMed)33611157

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a xxu

100    1_

$a Yue, Yanan $u School of Life, Jiangsu Normal University, Shanghai Road 101, 221116 Xuzhou, China

245    14

$a The fate of anaerobic syntrophy in anaerobic digestion facing propionate and acetate accumulation / $c Y. Yue, J. Wang, X. Wu, J. Zhang, Z. Chen, X. Kang, Z. Lv

520    9_

$a How the acetate and propionate accumulation impact anaerobic syntrophy during methane formation is not well understood. To investigate such effect, continuous acetate (35 g/L), propionate (11.25 g/L) and bicarbonate (30 g/L) supplementation were used during mesophilic anaerobic digestion. The high throughput sequencing (16S rRNA and mcrA), Real-Time quantitative PCR, and stable carbon isotope fingerprinting were applied to investigate the structure and activity of microbial community members. The results demonstrated that the abundance of syntrophic acetate oxidizing bacteria exhibited a gradual decrease coupled with heavier stable carbon isotopic signature of methane (δ 13CH4) in the three reagents impacted reactors. The increased acetate and propionate concentrations exerted negative influence on biogas production but the relatively stable hydrogenotrophic methanogens together with syntrophic acetate/propionate oxidizing bacteria kept the stable methane formation facing acetate and propionate accumulation. The functional genes copy number of the hydrogenotrophic Methanocellaceae and Methanomicrobiaceae correlated significantly with δ 13CH4 (R2 > 0.74), but only the abundance of Methanocellaceae fitted well with δ 13CH4 (p < 0.05). The δ 13CH4 signatures can predict methanogenesis, as it directly reflects the main methanogenic pathway; yet, further investigation of isotope fractionation in acetate/propionate coupled with δ 13CH4 is needed. Collectively, these results provide deep insight into anaerobic syntrophy and reveal changes of synergistic relationships, both of which may contribute to the stability of biogas reactors.

650    _2

$a acetáty $7 D000085

650    _2

$a anaerobióza $7 D000693

650    12

$a bioreaktory $7 D019149

650    _2

$a methan $7 D008697

650    12

$a propionáty $7 D011422

650    _2

$a RNA ribozomální 16S $x genetika $7 D012336

655    _2

$a časopisecké články $7 D016428

700    1_

$a Wang, Junyu $u School of Life, Jiangsu Normal University, Shanghai Road 101, 221116 Xuzhou, China

700    1_

$a Wu, Xiayuan $u Bioenergy Research Institute, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China

700    1_

$a Zhang, Jianfeng $u Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China

700    1_

$a Chen, Zhongbing $u Department of Applied Ecology, Faculty of Environmental Sciences, Czech University of Life Sciences Prague, Kamýcká 129, 16500 Prague, Czech Republic. Electronic address: chenz@fzp.czu.cz

700    1_

$a Kang, Xuejing $u Department of Applied Ecology, Faculty of Environmental Sciences, Czech University of Life Sciences Prague, Kamýcká 129, 16500 Prague, Czech Republic

700    1_

$a Lv, Zuopeng $u School of Life, Jiangsu Normal University, Shanghai Road 101, 221116 Xuzhou, China. Electronic address: zuopeng.lv@jsnu.edu.cn

773    0_

$w MED00006459 $t Waste management (New York, N.Y.) $x 1879-2456 $g Roč. 124, č. - (2021), s. 128-135

856    41

$u https://pubmed.ncbi.nlm.nih.gov/33611157 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y p $z 0

990    __

$a 20210420 $b ABA008

991    __

$a 20210507105120 $b ABA008

999    __

$a ok $b bmc $g 1649951 $s 1131796

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2021 $b 124 $c - $d 128-135 $e 20210219 $i 1879-2456 $m Waste management $n Waste Manag $x MED00006459

LZP    __

$a Pubmed-20210420