• Something wrong with this record ?

Exon junction complex dependent mRNA localization is linked to centrosome organization during ciliogenesis

OS. Kwon, R. Mishra, A. Safieddine, E. Coleno, Q. Alasseur, M. Faucourt, I. Barbosa, E. Bertrand, N. Spassky, H. Le Hir

. 2021 ; 12 (1) : 1351. [pub] 20210301

Language English Country Great Britain

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc21011468

Exon junction complexes (EJCs) mark untranslated spliced mRNAs and are crucial for the mRNA lifecycle. An imbalance in EJC dosage alters mouse neural stem cell (mNSC) division and is linked to human neurodevelopmental disorders. In quiescent mNSC and immortalized human retinal pigment epithelial (RPE1) cells, centrioles form a basal body for ciliogenesis. Here, we report that EJCs accumulate at basal bodies of mNSC or RPE1 cells and decline when these cells differentiate or resume growth. A high-throughput smFISH screen identifies two transcripts accumulating at centrosomes in quiescent cells, NIN and BICD2. In contrast to BICD2, the localization of NIN transcripts is EJC-dependent. NIN mRNA encodes a core component of centrosomes required for microtubule nucleation and anchoring. We find that EJC down-regulation impairs both pericentriolar material organization and ciliogenesis. An EJC-dependent mRNA trafficking towards centrosome and basal bodies might contribute to proper mNSC division and brain development.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21011468
003      
CZ-PrNML
005      
20210714101520.0
007      
ta
008      
210420s2021 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1038/s41467-021-21590-w $2 doi
035    __
$a (PubMed)33649372
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Kwon, Oh Sung $u Institut de Biologie de l'Ecole Normale Supérieure (IBENS), Ecole Normale Supérieure, CNRS, INSERM, PSL Research University, Paris, France
245    10
$a Exon junction complex dependent mRNA localization is linked to centrosome organization during ciliogenesis / $c OS. Kwon, R. Mishra, A. Safieddine, E. Coleno, Q. Alasseur, M. Faucourt, I. Barbosa, E. Bertrand, N. Spassky, H. Le Hir
520    9_
$a Exon junction complexes (EJCs) mark untranslated spliced mRNAs and are crucial for the mRNA lifecycle. An imbalance in EJC dosage alters mouse neural stem cell (mNSC) division and is linked to human neurodevelopmental disorders. In quiescent mNSC and immortalized human retinal pigment epithelial (RPE1) cells, centrioles form a basal body for ciliogenesis. Here, we report that EJCs accumulate at basal bodies of mNSC or RPE1 cells and decline when these cells differentiate or resume growth. A high-throughput smFISH screen identifies two transcripts accumulating at centrosomes in quiescent cells, NIN and BICD2. In contrast to BICD2, the localization of NIN transcripts is EJC-dependent. NIN mRNA encodes a core component of centrosomes required for microtubule nucleation and anchoring. We find that EJC down-regulation impairs both pericentriolar material organization and ciliogenesis. An EJC-dependent mRNA trafficking towards centrosome and basal bodies might contribute to proper mNSC division and brain development.
650    _2
$a zvířata $7 D000818
650    _2
$a autoantigeny $x metabolismus $7 D001324
650    _2
$a buněčný cyklus $7 D002453
650    _2
$a proteiny buněčného cyklu $x metabolismus $7 D018797
650    _2
$a proliferace buněk $7 D049109
650    _2
$a centrozom $x metabolismus $7 D018385
650    _2
$a cilie $x metabolismus $7 D002923
650    _2
$a cytoskeletální proteiny $x metabolismus $7 D003598
650    _2
$a DEAD-box RNA-helikasy $x metabolismus $7 D053487
650    _2
$a eukaryotický iniciační faktor 4A $x metabolismus $7 D039601
650    _2
$a exony $x genetika $7 D005091
650    _2
$a lidé $7 D006801
650    _2
$a myši $7 D051379
650    _2
$a proteiny asociované s mikrotubuly $x metabolismus $7 D008869
650    _2
$a mikrotubuly $x metabolismus $7 D008870
650    _2
$a nervové kmenové buňky $x metabolismus $7 D058953
650    _2
$a jaderné proteiny $x metabolismus $7 D009687
650    _2
$a proteosyntéza $7 D014176
650    12
$a transport RNA $7 D034443
650    _2
$a messenger RNA $x metabolismus $7 D012333
650    _2
$a proteiny vázající RNA $x metabolismus $7 D016601
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Mishra, Rahul $u Institut de Biologie de l'Ecole Normale Supérieure (IBENS), Ecole Normale Supérieure, CNRS, INSERM, PSL Research University, Paris, France ; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, Ceske Budejovice, Czech Republic
700    1_
$a Safieddine, Adham $u Institut de Génétique Moléculaire de Montpellier, University of Montpellier, CNRS, Montpellier, France ; Equipe labélisée Ligue Nationale Contre le Cancer, University of Montpellier, CNRS, Montpellier, France
700    1_
$a Coleno, Emeline $u Institut de Génétique Moléculaire de Montpellier, University of Montpellier, CNRS, Montpellier, France ; Equipe labélisée Ligue Nationale Contre le Cancer, University of Montpellier, CNRS, Montpellier, France
700    1_
$a Alasseur, Quentin $u Institut de Biologie de l'Ecole Normale Supérieure (IBENS), Ecole Normale Supérieure, CNRS, INSERM, PSL Research University, Paris, France
700    1_
$a Faucourt, Marion $u Institut de Biologie de l'Ecole Normale Supérieure (IBENS), Ecole Normale Supérieure, CNRS, INSERM, PSL Research University, Paris, France
700    1_
$a Barbosa, Isabelle $u Institut de Biologie de l'Ecole Normale Supérieure (IBENS), Ecole Normale Supérieure, CNRS, INSERM, PSL Research University, Paris, France
700    1_
$a Bertrand, Edouard $u Institut de Génétique Moléculaire de Montpellier, University of Montpellier, CNRS, Montpellier, France ; Equipe labélisée Ligue Nationale Contre le Cancer, University of Montpellier, CNRS, Montpellier, France
700    1_
$a Spassky, Nathalie $u Institut de Biologie de l'Ecole Normale Supérieure (IBENS), Ecole Normale Supérieure, CNRS, INSERM, PSL Research University, Paris, France
700    1_
$a Le Hir, Hervé $u Institut de Biologie de l'Ecole Normale Supérieure (IBENS), Ecole Normale Supérieure, CNRS, INSERM, PSL Research University, Paris, France. lehir@ens.fr
773    0_
$w MED00184850 $t Nature communications $x 2041-1723 $g Roč. 12, č. 1 (2021), s. 1351
856    41
$u https://pubmed.ncbi.nlm.nih.gov/33649372 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20210420 $b ABA008
991    __
$a 20210714101518 $b ABA008
999    __
$a ok $b bmc $g 1649991 $s 1131847
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 12 $c 1 $d 1351 $e 20210301 $i 2041-1723 $m Nature communications $n Nat Commun $x MED00184850
LZP    __
$a Pubmed-20210420

Find record

Citation metrics

Archiving options