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The molecular structure of mammalian primary cilia revealed by cryo-electron tomography
P. Kiesel, G. Alvarez Viar, N. Tsoy, R. Maraspini, P. Gorilak, V. Varga, A. Honigmann, G. Pigino
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
ProQuest Central
from 2004-01-01 to 1 year ago
Health & Medicine (ProQuest)
from 2004-01-01 to 1 year ago
- MeSH
- Cell Culture Techniques MeSH
- Madin Darby Canine Kidney Cells MeSH
- Chlamydomonas metabolism ultrastructure MeSH
- Cilia metabolism ultrastructure MeSH
- Cryoelectron Microscopy MeSH
- Gene Expression MeSH
- Humans MeSH
- Actin Cytoskeleton metabolism ultrastructure MeSH
- Microtubules metabolism ultrastructure MeSH
- Microtubule-Associated Proteins genetics metabolism MeSH
- Dogs MeSH
- Electron Microscope Tomography MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Dogs MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Primary cilia are microtubule-based organelles that are important for signaling and sensing in eukaryotic cells. Unlike the thoroughly studied motile cilia, the three-dimensional architecture and molecular composition of primary cilia are largely unexplored. Yet, studying these aspects is necessary to understand how primary cilia function in health and disease. We developed an enabling method for investigating the structure of primary cilia isolated from MDCK-II cells at molecular resolution by cryo-electron tomography. We show that the textbook '9 + 0' arrangement of microtubule doublets is only present at the primary cilium base. A few microns out, the architecture changes into an unstructured bundle of EB1-decorated microtubules and actin filaments, putting an end to a long debate on the presence or absence of actin filaments in primary cilia. Our work provides a plethora of insights into the molecular structure of primary cilia and offers a methodological framework to study these important organelles.
Institute of Molecular Genetics of the Czech Academy of Sciences Prague Czech Republic
Max Planck Institute of Molecular Cell Biology and Genetics Dresden Germany
References provided by Crossref.org
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