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A combined transcriptomic approach to identify candidates for an anti-tick vaccine blocking B. afzelii transmission
JJA. Trentelman, R. Sima, N. Krezdorn, J. Tomás-Cortázar, D. Barriales, K. Takumi, JM. Butler, H. Sprong, MJ. Klouwens, V. Urbanova, S. Mahmood, P. Winter, P. Kopacek, J. Anguita, O. Hajdusek, JW. Hovius
Jazyk angličtina Země Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
Free Medical Journals od 2011
Nature Open Access od 2011-12-01
PubMed Central od 2011
Europe PubMed Central od 2011
ProQuest Central od 2011-01-01
Open Access Digital Library od 2011-01-01
Open Access Digital Library od 2011-01-01
Health & Medicine (ProQuest) od 2011-01-01
ROAD: Directory of Open Access Scholarly Resources od 2011
Odkazy
PubMed
33208766
DOI
10.1038/s41598-020-76268-y
Knihovny.cz E-zdroje
- MeSH
- arachnida jako vektory mikrobiologie MeSH
- bakteriální vakcíny aplikace a dávkování MeSH
- Borrelia burgdorferi komplex účinky léků MeSH
- infestace klíšťaty genetika mikrobiologie prevence a kontrola přenos MeSH
- klíště účinky léků MeSH
- lymeská nemoc genetika mikrobiologie prevence a kontrola přenos MeSH
- myši MeSH
- proteiny členovců genetika MeSH
- slinné žlázy mikrobiologie MeSH
- transkriptom * MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Ixodes ricinus is the vector for Borrelia afzelii, the predominant cause of Lyme borreliosis in Europe, whereas Ixodes scapularis is the vector for Borrelia burgdorferi in the USA. Transcription of several I. scapularis genes changes in the presence of B. burgdorferi and contributes to successful infection. To what extend B. afzelii influences gene expression in I. ricinus salivary glands is largely unknown. Therefore, we measured expression of uninfected vs. infected tick salivary gland genes during tick feeding using Massive Analysis of cDNA Ends (MACE) and RNAseq, quantifying 26.179 unique transcripts. While tick feeding was the main differentiator, B. afzelii infection significantly affected expression of hundreds of transcripts, including 465 transcripts after 24 h of tick feeding. Validation of the top-20 B. afzelii-upregulated transcripts at 24 h of tick feeding in ten biological genetic distinct replicates showed that expression varied extensively. Three transcripts could be validated, a basic tail protein, a lipocalin and an ixodegrin, and might be involved in B. afzelii transmission. However, vaccination with recombinant forms of these proteins only marginally altered B. afzelii infection in I. ricinus-challenged mice for one of the proteins. Collectively, our data show that identification of tick salivary genes upregulated in the presence of pathogens could serve to identify potential pathogen-blocking vaccine candidates.
Biology Centre Institute of Parasitology Czech Academy of Sciences Ceske Budejovice Czech Republic
CIC bioGUNE Basque Research and Technology Alliance 48160 Derio Spain
Faculty of Science University of South Bohemia Ceske Budejovice Czech Republic
GenXPro GmbH Frankfurt Innovation Center Biotechnology Frankfurt am Main Germany
Ikerbasque Basque Foundation for Science 48012 Bilbao Spain
National Institute for Public Health and the Environment Bilthoven The Netherlands
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