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Tick-Borne Encephalitis Virus Adaptation in Different Host Environments and Existence of Quasispecies

R. Helmová, V. Hönig, H. Tykalová, M. Palus, L. Bell-Sakyi, L. Grubhoffer

. 2020 ; 12 (8) : . [pub] 20200818

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc21012257

Grantová podpora
BB/P024270/1 Biotechnology and Biological Sciences Research Council - United Kingdom

A highly virulent strain (Hypr) of tick-borne encephalitis virus (TBEV) was serially subcultured in the mammalian porcine kidney stable (PS) and Ixodes ricinus tick (IRE/CTVM19) cell lines, producing three viral variants. These variants exhibited distinct plaque sizes and virulence in a mouse model. Comparing the full-genome sequences of all variants, several nucleotide changes were identified in different genomic regions. Furthermore, different sequential variants were revealed to co-exist within one sample as quasispecies. Interestingly, the above-mentioned nucleotide changes found within the whole genome sequences of the new variants were present alongside the nucleotide sequence of the parental strain, which was represented as a minority quasispecies. These observations further imply that TBEV exists as a heterogeneous population that contains virus variants pre-adapted to reproduction in different environments, probably enabling virus survival in ticks and mammals.

Citace poskytuje Crossref.org

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