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PRP8A and PRP8B spliceosome subunits act coordinately to control pollen tube attraction in Arabidopsis thaliana
K. Kulichová, V. Kumar, L. Steinbachová, B. Klodová, L. Timofejeva, M. Juříček, D. Honys, SS. Hafidh
Language English Country Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Free Medical Journals
from 1953 to 6 months ago
Open Access Digital Library
from 1953-03-01 to 6 months ago
PubMed
32345744
DOI
10.1242/dev.186742
Knihovny.cz E-resources
- MeSH
- Arabidopsis metabolism MeSH
- Microscopy, Fluorescence MeSH
- Plants, Genetically Modified metabolism MeSH
- RNA Splice Sites MeSH
- Mutagenesis MeSH
- Protein Subunits genetics metabolism MeSH
- Arabidopsis Proteins chemistry genetics metabolism MeSH
- RNA-Binding Proteins chemistry genetics metabolism MeSH
- Pollen Tube growth & development metabolism MeSH
- Gene Expression Regulation, Plant MeSH
- Amino Acid Sequence MeSH
- Sequence Alignment MeSH
- RNA Splicing Factors genetics metabolism MeSH
- Spliceosomes metabolism MeSH
- Transcription Factors genetics metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Precise guided pollen tube growth by the female gametophyte is a prerequisite for successful sexual reproduction in flowering plants. Cysteine-rich proteins (CRPs) secreted from the embryo sac are known pollen tube attractants perceived by pollen tube receptor-like kinases. How pre-mRNA splicing facilitates this cell-to-cell communication is not understood. Here, we report a novel function of Pre-mRNA PROCESSING factor 8 paralogs, PRP8A and PRP8B, as regulators of pollen tube attraction. Double mutant prp8a prp8b ovules cannot attract pollen tubes, and prp8a prp8b pollen tubes fail to sense the ovule's attraction signals. Only 3% of ovule-expressed genes were misregulated in prp8a prp8b Combination of RNA sequencing and the MYB98/LURE1.2-YFP reporter revealed that the expression of MYB98, LUREs and 49 other CRPs were downregulated, suggesting loss of synergid cell fate. Differential exon usage and intron retention analysis revealed autoregulation of PPR8A/PRP8B splicing. In vivo, PRP8A co-immunoprecipitates with splicing enhancer AtSF3A1, suggesting involvement of PRP8A in 3'-splice site selection. Our data hint that the PRP8A/PRP8B module exhibits spliceosome autoregulation to facilitate pollen tube attraction via transcriptional regulation of MYB98, CRPs and LURE pollen tube attractants.
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