Precise guided pollen tube growth by the female gametophyte is a prerequisite for successful sexual reproduction in flowering plants. Cysteine-rich proteins (CRPs) secreted from the embryo sac are known pollen tube attractants perceived by pollen tube receptor-like kinases. How pre-mRNA splicing facilitates this cell-to-cell communication is not understood. Here, we report a novel function of Pre-mRNA PROCESSING factor 8 paralogs, PRP8A and PRP8B, as regulators of pollen tube attraction. Double mutant prp8a prp8b ovules cannot attract pollen tubes, and prp8a prp8b pollen tubes fail to sense the ovule's attraction signals. Only 3% of ovule-expressed genes were misregulated in prp8a prp8b Combination of RNA sequencing and the MYB98/LURE1.2-YFP reporter revealed that the expression of MYB98, LUREs and 49 other CRPs were downregulated, suggesting loss of synergid cell fate. Differential exon usage and intron retention analysis revealed autoregulation of PPR8A/PRP8B splicing. In vivo, PRP8A co-immunoprecipitates with splicing enhancer AtSF3A1, suggesting involvement of PRP8A in 3'-splice site selection. Our data hint that the PRP8A/PRP8B module exhibits spliceosome autoregulation to facilitate pollen tube attraction via transcriptional regulation of MYB98, CRPs and LURE pollen tube attractants.

Department of Plant Experimental Biology Faculty of Science Charles University Viničná 5 128 44 Prague 2 Czech Republic

Institute of Experimental Botany of the Czech Academy of Sciences Rozvojová 263 165 02 Prague 6 Czech Republic

000      

00000naa a2200000 a 4500

001      

bmc21012475

003      

CZ-PrNML

005      

20210507102003.0

007      

ta

008      

210420s2020 xxk f 000 0|eng||

009      

AR

024    7_

$a 10.1242/dev.186742 $2 doi

035    __

$a (PubMed)32345744

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a xxk

100    1_

$a Kulichová, Katarína $u Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Prague 6, Czech Republic

245    10

$a PRP8A and PRP8B spliceosome subunits act coordinately to control pollen tube attraction in Arabidopsis thaliana / $c K. Kulichová, V. Kumar, L. Steinbachová, B. Klodová, L. Timofejeva, M. Juříček, D. Honys, SS. Hafidh

520    9_

$a Precise guided pollen tube growth by the female gametophyte is a prerequisite for successful sexual reproduction in flowering plants. Cysteine-rich proteins (CRPs) secreted from the embryo sac are known pollen tube attractants perceived by pollen tube receptor-like kinases. How pre-mRNA splicing facilitates this cell-to-cell communication is not understood. Here, we report a novel function of Pre-mRNA PROCESSING factor 8 paralogs, PRP8A and PRP8B, as regulators of pollen tube attraction. Double mutant prp8a prp8b ovules cannot attract pollen tubes, and prp8a prp8b pollen tubes fail to sense the ovule's attraction signals. Only 3% of ovule-expressed genes were misregulated in prp8a prp8b Combination of RNA sequencing and the MYB98/LURE1.2-YFP reporter revealed that the expression of MYB98, LUREs and 49 other CRPs were downregulated, suggesting loss of synergid cell fate. Differential exon usage and intron retention analysis revealed autoregulation of PPR8A/PRP8B splicing. In vivo, PRP8A co-immunoprecipitates with splicing enhancer AtSF3A1, suggesting involvement of PRP8A in 3'-splice site selection. Our data hint that the PRP8A/PRP8B module exhibits spliceosome autoregulation to facilitate pollen tube attraction via transcriptional regulation of MYB98, CRPs and LURE pollen tube attractants.

650    _2

$a sekvence aminokyselin $7 D000595

650    _2

$a Arabidopsis $x metabolismus $7 D017360

650    _2

$a proteiny huseníčku $x chemie $x genetika $x metabolismus $7 D029681

650    _2

$a regulace genové exprese u rostlin $7 D018506

650    _2

$a fluorescenční mikroskopie $7 D008856

650    _2

$a mutageneze $7 D016296

650    _2

$a geneticky modifikované rostliny $x metabolismus $7 D030821

650    _2

$a pylová láčka $x růst a vývoj $x metabolismus $7 D053205

650    _2

$a podjednotky proteinů $x genetika $x metabolismus $7 D021122

650    _2

$a místa sestřihu RNA $7 D022821

650    _2

$a sestřihové faktory $x genetika $x metabolismus $7 D000072260

650    _2

$a proteiny vázající RNA $x chemie $x genetika $x metabolismus $7 D016601

650    _2

$a sekvenční seřazení $7 D016415

650    _2

$a spliceozomy $x metabolismus $7 D017381

650    _2

$a transkripční faktory $x genetika $x metabolismus $7 D014157

655    _2

$a časopisecké články $7 D016428

655    _2

$a práce podpořená grantem $7 D013485

700    1_

$a Kumar, Vinod $u Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Prague 6, Czech Republic

700    1_

$a Steinbachová, Lenka $u Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Prague 6, Czech Republic

700    1_

$a Klodová, Božena $u Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Prague 6, Czech Republic

700    1_

$a Timofejeva, Ljudmilla $u Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Prague 6, Czech Republic

700    1_

$a Juříček, Miloslav $u Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Prague 6, Czech Republic

700    1_

$a Honys, David $u Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Prague 6, Czech Republic $u Department of Plant Experimental Biology, Faculty of Science, Charles University, Viničná 5, 128 44 Prague 2, Czech Republic

700    1_

$a Hafidh, Said S $u Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Prague 6, Czech Republic hafidh@ueb.cas.cz

773    0_

$w MED00001363 $t Development (Cambridge, England) $x 1477-9129 $g Roč. 147, č. 11 (2020)

856    41

$u https://pubmed.ncbi.nlm.nih.gov/32345744 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y p $z 0

990    __

$a 20210420 $b ABA008

991    __

$a 20210507102002 $b ABA008

999    __

$a ok $b bmc $g 1650772 $s 1132854

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2020 $b 147 $c 11 $e 20200614 $i 1477-9129 $m Development $n Development $x MED00001363

LZP    __

$a Pubmed-20210420