• Je něco špatně v tomto záznamu ?

Circulation of Babesia Species and Their Exposure to Humans through Ixodes Ricinus

T. Azagi, RI. Jaarsma, A. Docters van Leeuwen, M. Fonville, M. Maas, FFJ. Franssen, M. Kik, JM. Rijks, MG. Montizaan, M. Groenevelt, M. Hoyer, HJ. Esser, AI. Krawczyk, D. Modrý, H. Sprong, S. Demir

. 2021 ; 10 (4) : . [pub] 20210324

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc21018107

Grantová podpora
522003007 ZonMw - Netherlands

Human babesiosis in Europe has been attributed to infection with Babesia divergens and, to a lesser extent, with Babesia venatorum and Babesia microti, which are all transmitted to humans through a bite of Ixodes ricinus. These Babesia species circulate in the Netherlands, but autochthonous human babesiosis cases have not been reported so far. To gain more insight into the natural sources of these Babesia species, their presence in reservoir hosts and in I. ricinus was examined. Moreover, part of the ticks were tested for co-infections with other tick borne pathogens. In a cross-sectional study, qPCR-detection was used to determine the presence of Babesia species in 4611 tissue samples from 27 mammalian species and 13 bird species. Reverse line blotting (RLB) and qPCR detection of Babesia species were used to test 25,849 questing I. ricinus. Fragments of the 18S rDNA and cytochrome c oxidase subunit I (COI) gene from PCR-positive isolates were sequenced for confirmation and species identification and species-specific PCR reactions were performed on samples with suspected mixed infections. Babesia microti was found in two widespread rodent species: Myodes glareolus and Apodemus sylvaticus, whereas B. divergens was detected in the geographically restricted Cervus elaphus and Bison bonasus, and occasionally in free-ranging Ovis aries. B. venatorum was detected in the ubiquitous Capreolus capreolus, and occasionally in free-ranging O. aries. Species-specific PCR revealed co-infections in C. capreolus and C. elaphus, resulting in higher prevalence of B. venatorum and B. divergens than disclosed by qPCR detection, followed by 18S rDNA and COI sequencing. The non-zoonotic Babesia species found were Babesia capreoli, Babesia vulpes, Babesia sp. deer clade, and badger-associated Babesia species. The infection rate of zoonotic Babesia species in questing I. ricinus ticks was higher for Babesia clade I (2.6%) than Babesia clade X (1.9%). Co-infection of B. microti with Borrelia burgdorferi sensu lato and Neoehrlichia mikurensis in questing nymphs occurred more than expected, which reflects their mutual reservoir hosts, and suggests the possibility of co-transmission of these three pathogens to humans during a tick bite. The ubiquitous spread and abundance of B. microti and B. venatorum in their reservoir hosts and questing ticks imply some level of human exposure through tick bites. The restricted distribution of the wild reservoir hosts for B. divergens and its low infection rate in ticks might contribute to the absence of reported autochthonous cases of human babesiosis in the Netherlands.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21018107
003      
CZ-PrNML
005      
20210729104132.0
007      
ta
008      
210726s2021 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/pathogens10040386 $2 doi
035    __
$a (PubMed)33804875
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Azagi, Tal $u Centre for Infectious Disease Control, National Institute for Public Health and the Environment, 3720 BA Bilthoven, The Netherlands
245    10
$a Circulation of Babesia Species and Their Exposure to Humans through Ixodes Ricinus / $c T. Azagi, RI. Jaarsma, A. Docters van Leeuwen, M. Fonville, M. Maas, FFJ. Franssen, M. Kik, JM. Rijks, MG. Montizaan, M. Groenevelt, M. Hoyer, HJ. Esser, AI. Krawczyk, D. Modrý, H. Sprong, S. Demir
520    9_
$a Human babesiosis in Europe has been attributed to infection with Babesia divergens and, to a lesser extent, with Babesia venatorum and Babesia microti, which are all transmitted to humans through a bite of Ixodes ricinus. These Babesia species circulate in the Netherlands, but autochthonous human babesiosis cases have not been reported so far. To gain more insight into the natural sources of these Babesia species, their presence in reservoir hosts and in I. ricinus was examined. Moreover, part of the ticks were tested for co-infections with other tick borne pathogens. In a cross-sectional study, qPCR-detection was used to determine the presence of Babesia species in 4611 tissue samples from 27 mammalian species and 13 bird species. Reverse line blotting (RLB) and qPCR detection of Babesia species were used to test 25,849 questing I. ricinus. Fragments of the 18S rDNA and cytochrome c oxidase subunit I (COI) gene from PCR-positive isolates were sequenced for confirmation and species identification and species-specific PCR reactions were performed on samples with suspected mixed infections. Babesia microti was found in two widespread rodent species: Myodes glareolus and Apodemus sylvaticus, whereas B. divergens was detected in the geographically restricted Cervus elaphus and Bison bonasus, and occasionally in free-ranging Ovis aries. B. venatorum was detected in the ubiquitous Capreolus capreolus, and occasionally in free-ranging O. aries. Species-specific PCR revealed co-infections in C. capreolus and C. elaphus, resulting in higher prevalence of B. venatorum and B. divergens than disclosed by qPCR detection, followed by 18S rDNA and COI sequencing. The non-zoonotic Babesia species found were Babesia capreoli, Babesia vulpes, Babesia sp. deer clade, and badger-associated Babesia species. The infection rate of zoonotic Babesia species in questing I. ricinus ticks was higher for Babesia clade I (2.6%) than Babesia clade X (1.9%). Co-infection of B. microti with Borrelia burgdorferi sensu lato and Neoehrlichia mikurensis in questing nymphs occurred more than expected, which reflects their mutual reservoir hosts, and suggests the possibility of co-transmission of these three pathogens to humans during a tick bite. The ubiquitous spread and abundance of B. microti and B. venatorum in their reservoir hosts and questing ticks imply some level of human exposure through tick bites. The restricted distribution of the wild reservoir hosts for B. divergens and its low infection rate in ticks might contribute to the absence of reported autochthonous cases of human babesiosis in the Netherlands.
655    _2
$a časopisecké články $7 D016428
700    1_
$a Jaarsma, Ryanne I $u Centre for Infectious Disease Control, National Institute for Public Health and the Environment, 3720 BA Bilthoven, The Netherlands
700    1_
$a Docters van Leeuwen, Arieke $u Centre for Infectious Disease Control, National Institute for Public Health and the Environment, 3720 BA Bilthoven, The Netherlands
700    1_
$a Fonville, Manoj $u Centre for Infectious Disease Control, National Institute for Public Health and the Environment, 3720 BA Bilthoven, The Netherlands
700    1_
$a Maas, Miriam $u Centre for Infectious Disease Control, National Institute for Public Health and the Environment, 3720 BA Bilthoven, The Netherlands
700    1_
$a Franssen, Frits F J $u Centre for Infectious Disease Control, National Institute for Public Health and the Environment, 3720 BA Bilthoven, The Netherlands
700    1_
$a Kik, Marja $u Dutch Wildlife Health Centre, Utrecht University, 3584 CL Utrecht, The Netherlands
700    1_
$a Rijks, Jolianne M $u Dutch Wildlife Health Centre, Utrecht University, 3584 CL Utrecht, The Netherlands
700    1_
$a Montizaan, Margriet G $u Dutch Wildlife Health Centre, Utrecht University, 3584 CL Utrecht, The Netherlands
700    1_
$a Groenevelt, Margit $u Diergeneeskundig Centrum Zuid-Oost Drenthe, 7741 EE Coevorden, The Netherlands
700    1_
$a Hoyer, Mark $u Veterinair en Immobilisatie Adviesbureau, 1697 KW Schellinkhout, The Netherlands
700    1_
$a Esser, Helen J $u Wildlife Ecology & Conservation Group, Wageningen University, 6708 PB Wageningen, The Netherlands
700    1_
$a Krawczyk, Aleksandra I $u Centre for Infectious Disease Control, National Institute for Public Health and the Environment, 3720 BA Bilthoven, The Netherlands $u Laboratory of Entomology, Wageningen University, 6708 PB Wageningen, The Netherlands
700    1_
$a Modrý, David $u Institute of Parasitology, Biology Centre CAS, 370 05 Ceske Budejovice, Czech Republic $u Department of Botany and Zoology, Faculty of Science, Masaryk University, 611 37 Brno, Czech Republic $u Department of Veterinary Sciences/CINeZ, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, 165 00 Prague, Czech Republic
700    1_
$a Sprong, Hein $u Centre for Infectious Disease Control, National Institute for Public Health and the Environment, 3720 BA Bilthoven, The Netherlands $u Laboratory of Entomology, Wageningen University, 6708 PB Wageningen, The Netherlands
700    1_
$a Demir, Samiye $u Centre for Infectious Disease Control, National Institute for Public Health and the Environment, 3720 BA Bilthoven, The Netherlands
773    0_
$w MED00195469 $t Pathogens (Basel, Switzerland) $x 2076-0817 $g Roč. 10, č. 4 (2021)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/33804875 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20210726 $b ABA008
991    __
$a 20210729104131 $b ABA008
999    __
$a ind $b bmc $g 1676538 $s 1138551
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 10 $c 4 $e 20210324 $i 2076-0817 $m Pathogens $n Pathogens $x MED00195469
GRA    __
$a 522003007 $p ZonMw $2 Netherlands
LZP    __
$a Pubmed-20210726

Najít záznam

Citační ukazatele

Možnosti archivace