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Important parameters affecting quality of vitrified donor oocytes

O. Teplá, Z. Topurko, J. Mašata, S. Jirsová, M. Frolíková, K. Komrsková, A. Minks, J. Turánek, A. Lynnyk, I. Kratochvílová

. 2021 ; 100 (-) : 110-116. [pub] 20210305

Jazyk angličtina Země Nizozemsko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc21018498

For group of 281 oocytes obtained from 43 stimulated donors and cryopreserved by vitrification protocol using Cryotop and Kitazato medium we determined important parameters of oocytes collection and vitrification processes which strongly affect the probability that warmed oocytes will produce high-quality embryos for transfer. The probability to obtain high-quality embryos for transfer from vitrified and warmed oocytes was highest when two conditions were fulfilled: 1. oocytes were incubated before vitrification for 7-10 h and 2. stimulated ovaries of donors in one cycle produced a smaller number of oocytes (<7 oocytes from one donor per stimulated cycle). The probable reasons for these observations were: 1. early vitrification (less than 7 h) before final oocyte metaphase II maturation negatively affected the crucial process of post-warm remodelling of spindles and chromosomes, which reduced the fertilization and utilization rates, 2. the evaluated vitrification protocol amplifies negative impact of membrane defects of oocytes of those cohorts containing more than 6 oocytes - freezing places great demands on the integrity and elasticity of the cell membranes. The fact that cryopreservation influences a complex state of oocytes was confirmed by confocal microscopy.

Citace poskytuje Crossref.org

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$a Important parameters affecting quality of vitrified donor oocytes / $c O. Teplá, Z. Topurko, J. Mašata, S. Jirsová, M. Frolíková, K. Komrsková, A. Minks, J. Turánek, A. Lynnyk, I. Kratochvílová
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$a For group of 281 oocytes obtained from 43 stimulated donors and cryopreserved by vitrification protocol using Cryotop and Kitazato medium we determined important parameters of oocytes collection and vitrification processes which strongly affect the probability that warmed oocytes will produce high-quality embryos for transfer. The probability to obtain high-quality embryos for transfer from vitrified and warmed oocytes was highest when two conditions were fulfilled: 1. oocytes were incubated before vitrification for 7-10 h and 2. stimulated ovaries of donors in one cycle produced a smaller number of oocytes (<7 oocytes from one donor per stimulated cycle). The probable reasons for these observations were: 1. early vitrification (less than 7 h) before final oocyte metaphase II maturation negatively affected the crucial process of post-warm remodelling of spindles and chromosomes, which reduced the fertilization and utilization rates, 2. the evaluated vitrification protocol amplifies negative impact of membrane defects of oocytes of those cohorts containing more than 6 oocytes - freezing places great demands on the integrity and elasticity of the cell membranes. The fact that cryopreservation influences a complex state of oocytes was confirmed by confocal microscopy.
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$a Topurko, Zinovij $u Department of Obstetrics and Gynecology of the First Faculty of Medicine and General Teaching Hospital, Apolinářská 18, 128 51, Prague 2, Czech Republic. Electronic address: Zinovij.Topurko@vfn.cz
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$a Mašata, Jaromír $u Department of Obstetrics and Gynecology of the First Faculty of Medicine and General Teaching Hospital, Apolinářská 18, 128 51, Prague 2, Czech Republic. Electronic address: Jaromir.Masata@vfn.cz
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$a Jirsová, Simona $u Department of Obstetrics and Gynecology of the First Faculty of Medicine and General Teaching Hospital, Apolinářská 18, 128 51, Prague 2, Czech Republic. Electronic address: Simona.Jirsova@vfn.cz
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$a Frolíková, Michaela $u Laboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Průmyslová 595, 252 50, Vestec, Czech Republic. Electronic address: Michaela.Frolikova@ibt.cas.cz
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$a Komrsková, Kateřina $u Laboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Průmyslová 595, 252 50, Vestec, Czech Republic; Department of Zoology, Faculty of Science, Charles University, Vinicna 7, 128 44, Prague 2, Czech Republic. Electronic address: Katerina.Komrskova@ibt.cas.cz
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$a Minks, Adela $u ISCARE a.s, Českomoravská 2510/19, 190 00, Praha, Czech Republic. Electronic address: Minks@iscare.cz
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$a Turánek, Jaroslav $u Department of Immunology and Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc, 77515, Olomouc, Czech Republic. Electronic address: Turanek@seznam.cz
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$a Lynnyk, Anna $u Institute of Physics of the Czech Academy of Sciences, Na Slovance 2, CZ-182 21, Prague 8, Czech Republic. Electronic address: lynnyk@fzu.cz
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$a Kratochvílová, Irena $u Institute of Physics of the Czech Academy of Sciences, Na Slovance 2, CZ-182 21, Prague 8, Czech Republic. Electronic address: krat@fzu.cz
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