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The Effect of Chemical Structure of OEG Ligand Shells with Quaternary Ammonium Moiety on the Colloidal Stabilization, Cellular Uptake and Photothermal Stability of Gold Nanorods
S. Salajkova, F. Havel, M. Sramek, F. Novotny, D. Malinak, R. Dolezal, L. Prchal, M. Benkova, O. Soukup, K. Musilek, K. Kuca, J. Bartek, J. Proska, M. Zarska, Z. Hodny
Language English Country New Zealand
Document type Journal Article
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PubMed
34040371
DOI
10.2147/ijn.s304953
Knihovny.cz E-resources
- MeSH
- Biological Transport MeSH
- HeLa Cells MeSH
- Hydrophobic and Hydrophilic Interactions MeSH
- Colloids MeSH
- Quaternary Ammonium Compounds chemistry MeSH
- Humans MeSH
- Ligands MeSH
- Nanotubes chemistry MeSH
- Polyethylene Glycols chemistry MeSH
- Drug Stability MeSH
- Temperature * MeSH
- Gold chemistry metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
Purpose: Plasmonic photothermal cancer therapy by gold nanorods (GNRs) emerges as a promising tool for cancer treatment. The goal of this study was to design cationic oligoethylene glycol (OEG) compounds varying in hydrophobicity and molecular electrostatic potential as ligand shells of GNRs. Three series of ligands with different length of OEG chain (ethylene glycol units = 3, 4, 5) and variants of quaternary ammonium salts (QAS) as terminal functional group were synthesized and compared to a prototypical quaternary ammonium ligand with alkyl chain - (16-mercaptohexadecyl)trimethylammonium bromide (MTAB). Methods: Step-by-step research approach starting with the preparation of compounds characterized by NMR and HRMS spectra, GNRs ligand exchange evaluation through characterization of cytotoxicity and GNRs cellular uptake was used. A method quantifying the reshaping of GNRs was applied to determine the effect of ligand structure on the heat transport from GNRs under fs-laser irradiation. Results: Fourteen out of 18 synthesized OEG compounds successfully stabilized GNRs in the water. The colloidal stability of prepared GNRs in the cell culture medium decreased with the number of OEG units. In contrast, the cellular uptake of OEG+GNRs by HeLa cells increased with the length of OEG chain while the structure of the QAS group showed a minor role. Compared to MTAB, more hydrophilic OEG compounds exhibited nearly two order of magnitude lower cytotoxicity in free state and provided efficient cellular uptake of GNRs close to the level of MTAB. Regarding photothermal properties, OEG compounds evoked the photothermal reshaping of GNRs at lower peak fluence (14.8 mJ/cm2) of femtosecond laser irradiation than the alkanethiol MTAB. Conclusion: OEG+GNRs appear to be optimal for clinical applications with systemic administration of NPs not-requiring irradiation at high laser intensity such as drug delivery and photothermal therapy inducing apoptosis.
Biomedical Research Center University Hospital Hradec Kralove Hradec Kralove Czech Republic
Genome Integrity Unit Danish Cancer Society Research Center Copenhagen Denmark
References provided by Crossref.org
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