-
Je něco špatně v tomto záznamu ?
Influence of Lipid Membranes on α-Synuclein Aggregation
AS. Kurochka, DA. Yushchenko, P. Bouř, VV. Shvadchak
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- alfa-synuklein * MeSH
- amyloid MeSH
- kinetika MeSH
- lidé MeSH
- lipidy MeSH
- Parkinsonova nemoc * MeSH
- patologická konformace proteinů MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
α-Synuclein is a neuronal protein involved in synaptic vesicle trafficking. During the course of Parkinson's disease, it aggregates, forming amyloid fibrils that accumulate in the midbrain. This pathological fibrillization process is strongly modulated by physiological interactions of α-synuclein with lipid membranes. However, the detailed mechanism of this effect remains unclear. In this work, we used environment-sensitive fluorescent dyes to study the influence of model lipid membranes on the kinetics of α-synuclein fibrillization. We observed that formation of the fibrils from α-synuclein monomers is strongly delayed even by small amounts of lipids. Furthermore, we found that membrane-bound α-synuclein monomers are not involved in fibril elongation. Hence, presence of lipids slows down fibril growth proportionally to the fraction of membrane-bound protein.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc21019101
- 003
- CZ-PrNML
- 005
- 20210830100700.0
- 007
- ta
- 008
- 210728s2021 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1021/acschemneuro.0c00819 $2 doi
- 035 __
- $a (PubMed)33566579
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Kurochka, Andrii S $u Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry, Prague 166 10, Czech Republic $u Department of Analytical Chemistry, University of Chemistry and Technology, Prague 166 28, Czech Republic
- 245 10
- $a Influence of Lipid Membranes on α-Synuclein Aggregation / $c AS. Kurochka, DA. Yushchenko, P. Bouř, VV. Shvadchak
- 520 9_
- $a α-Synuclein is a neuronal protein involved in synaptic vesicle trafficking. During the course of Parkinson's disease, it aggregates, forming amyloid fibrils that accumulate in the midbrain. This pathological fibrillization process is strongly modulated by physiological interactions of α-synuclein with lipid membranes. However, the detailed mechanism of this effect remains unclear. In this work, we used environment-sensitive fluorescent dyes to study the influence of model lipid membranes on the kinetics of α-synuclein fibrillization. We observed that formation of the fibrils from α-synuclein monomers is strongly delayed even by small amounts of lipids. Furthermore, we found that membrane-bound α-synuclein monomers are not involved in fibril elongation. Hence, presence of lipids slows down fibril growth proportionally to the fraction of membrane-bound protein.
- 650 _2
- $a amyloid $7 D000682
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a kinetika $7 D007700
- 650 _2
- $a lipidy $7 D008055
- 650 12
- $a Parkinsonova nemoc $7 D010300
- 650 _2
- $a patologická konformace proteinů $7 D066263
- 650 12
- $a alfa-synuklein $7 D051844
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Yushchenko, Dmytro A $u Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry, Prague 166 10, Czech Republic $u Miltenyi Biotec GmbH, Bergisch Gladbach 51429, Germany
- 700 1_
- $a Bouř, Petr $u Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry, Prague 166 10, Czech Republic $u Department of Analytical Chemistry, University of Chemistry and Technology, Prague 166 28, Czech Republic
- 700 1_
- $a Shvadchak, Volodymyr V $u Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry, Prague 166 10, Czech Republic
- 773 0_
- $w MED00193636 $t ACS chemical neuroscience $x 1948-7193 $g Roč. 12, č. 5 (2021), s. 825-830
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/33566579 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20210728 $b ABA008
- 991 __
- $a 20210830100700 $b ABA008
- 999 __
- $a ok $b bmc $g 1690021 $s 1139547
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2021 $b 12 $c 5 $d 825-830 $e 20210210 $i 1948-7193 $m ACS chemical neuroscience $n ACS Chem Neurosci $x MED00193636
- LZP __
- $a Pubmed-20210728
