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Global and Site-Specific Effect of Phosphorylation on Protein Turnover

C. Wu, Q. Ba, D. Lu, W. Li, B. Salovska, P. Hou, T. Mueller, G. Rosenberger, E. Gao, Y. Di, H. Zhou, EF. Fornasiero, Y. Liu

. 2021 ; 56 (1) : 111-124.e6. [pub] 20201124

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc21019456

Grantová podpora
R01 GM137031 NIGMS NIH HHS - United States

E-zdroje Online Plný text

NLK Cell Press Free Archives od 2001-07-01 do Před 1 rokem
Free Medical Journals od 2001 do Před 1 rokem
Elsevier Open Access Journals od 2001-07-01 do 2023-06-19
Elsevier Open Archive Journals od 2001-07-01 do Před 1 rokem

Odkazy

PubMed 33238149
DOI 10.1016/j.devcel.2020.10.025
Knihovny.cz E-zdroje

To date, the effects of specific modification types and sites on protein lifetime have not been systematically illustrated. Here, we describe a proteomic method, DeltaSILAC, to quantitatively assess the impact of site-specific phosphorylation on the turnover of thousands of proteins in live cells. Based on the accurate and reproducible mass spectrometry-based method, a pulse labeling approach using stable isotope-labeled amino acids in cells (pSILAC), phosphoproteomics, and a unique peptide-level matching strategy, our DeltaSILAC profiling revealed a global, unexpected delaying effect of many phosphosites on protein turnover. We further found that phosphorylated sites accelerating protein turnover are functionally selected for cell fitness, enriched in Cyclin-dependent kinase substrates, and evolutionarily conserved, whereas the glutamic acids surrounding phosphosites significantly delay protein turnover. Our method represents a generalizable approach and provides a rich resource for prioritizing the effects of phosphorylation sites on protein lifetime in the context of cell signaling and disease biology.

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