-
Something wrong with this record ?
Virulence Factor Cargo and Host Cell Interactions of Shiga Toxin-Producing Escherichia coli Outer Membrane Vesicles
M. Bielaszewska, L. Greune, A. Bauwens, P. Dersch, A. Mellmann, C. Rüter
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Bacterial Toxins metabolism MeSH
- Endothelial Cells metabolism microbiology pathology MeSH
- Escherichia coli O157 * metabolism pathogenicity MeSH
- Virulence Factors metabolism MeSH
- Humans MeSH
- Cell-Derived Microparticles metabolism MeSH
- Shiga Toxin 2 metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Outer membrane vesicles (OMVs), nanoparticles released by Shiga toxin-producing Escherichia coli (STEC), have been identified as novel efficient virulence tools of these pathogens. STEC O157 OMVs carry a cocktail of virulence factors including Shiga toxin 2a (Stx2a), cytolethal distending toxin V (CdtV), EHEC hemolysin, flagellin, and lipopolysaccharide. OMVs are taken up by human intestinal epithelial and microvascular endothelial cells, the major targets during STEC infection, and deliver the virulence factors into host cells. There the toxins separate from OMVs and are trafficked via different pathways to their target compartments, i.e., the cytosol (Stx2a-A subunit), nucleus (CdtV-B subunit), and mitochondria (EHEC hemolysin). This leads to a toxin-specific host cell injury and ultimately apoptotic cell death. Besides their cytotoxic effects, STEC OMVs trigger an inflammatory response via their lipopolysaccharide and flagellin components. In this chapter, we describe methods for the isolation and purification of STEC OMVs, for the detection of OMV-associated virulence factors, and for the analysis of OMV interactions with host cells including OMV cellular uptake and intracellular trafficking of OMVs and OMV-delivered toxins.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc21019578
- 003
- CZ-PrNML
- 005
- 20210830101155.0
- 007
- ta
- 008
- 210728s2021 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/978-1-0716-1339-9_8 $2 doi
- 035 __
- $a (PubMed)33704754
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Bielaszewska, Martina $u National Reference Laboratory for E. coli and Shigellae, National Institute of Public Health, Prague, Czech Republic. martina.bielaszewska@szu.cz $u Institute for Hygiene, University of Münster, Münster, Germany. martina.bielaszewska@szu.cz
- 245 10
- $a Virulence Factor Cargo and Host Cell Interactions of Shiga Toxin-Producing Escherichia coli Outer Membrane Vesicles / $c M. Bielaszewska, L. Greune, A. Bauwens, P. Dersch, A. Mellmann, C. Rüter
- 520 9_
- $a Outer membrane vesicles (OMVs), nanoparticles released by Shiga toxin-producing Escherichia coli (STEC), have been identified as novel efficient virulence tools of these pathogens. STEC O157 OMVs carry a cocktail of virulence factors including Shiga toxin 2a (Stx2a), cytolethal distending toxin V (CdtV), EHEC hemolysin, flagellin, and lipopolysaccharide. OMVs are taken up by human intestinal epithelial and microvascular endothelial cells, the major targets during STEC infection, and deliver the virulence factors into host cells. There the toxins separate from OMVs and are trafficked via different pathways to their target compartments, i.e., the cytosol (Stx2a-A subunit), nucleus (CdtV-B subunit), and mitochondria (EHEC hemolysin). This leads to a toxin-specific host cell injury and ultimately apoptotic cell death. Besides their cytotoxic effects, STEC OMVs trigger an inflammatory response via their lipopolysaccharide and flagellin components. In this chapter, we describe methods for the isolation and purification of STEC OMVs, for the detection of OMV-associated virulence factors, and for the analysis of OMV interactions with host cells including OMV cellular uptake and intracellular trafficking of OMVs and OMV-delivered toxins.
- 650 _2
- $a bakteriální toxiny $x metabolismus $7 D001427
- 650 _2
- $a mikropartikule $x metabolismus $7 D055252
- 650 _2
- $a endoteliální buňky $x metabolismus $x mikrobiologie $x patologie $7 D042783
- 650 12
- $a Escherichia coli O157 $x metabolismus $x patogenita $7 D019453
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a shiga toxin 2 $x metabolismus $7 D022641
- 650 _2
- $a faktory virulence $x metabolismus $7 D037521
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Greune, Lilo $u Institute for Infectiology, Center for Molecular Biology of Inflammation (ZMBE), University of Münster, Münster, Germany
- 700 1_
- $a Bauwens, Andreas $u Institute for Hygiene, University of Münster, Münster, Germany
- 700 1_
- $a Dersch, Petra $u Institute for Infectiology, Center for Molecular Biology of Inflammation (ZMBE), University of Münster, Münster, Germany
- 700 1_
- $a Mellmann, Alexander $u Institute for Hygiene, University of Münster, Münster, Germany
- 700 1_
- $a Rüter, Christian $u Institute for Infectiology, Center for Molecular Biology of Inflammation (ZMBE), University of Münster, Münster, Germany
- 773 0_
- $w MED00180389 $t Methods in molecular biology (Clifton, N.J.) $x 1940-6029 $g Roč. 2291, č. - (2021), s. 177-205
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/33704754 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20210728 $b ABA008
- 991 __
- $a 20210830101155 $b ABA008
- 999 __
- $a ok $b bmc $g 1690418 $s 1140024
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2021 $b 2291 $c - $d 177-205 $e - $i 1940-6029 $m Methods in molecular biology $n Methods Mol Biol $x MED00180389
- LZP __
- $a Pubmed-20210728
