• Something wrong with this record ?

Mannan-Based Nanodiagnostic Agents for Targeting Sentinel Lymph Nodes and Tumors

M. Jirátová, A. Gálisová, M. Rabyk, E. Sticová, M. Hrubý, D. Jirák

. 2020 ; 26 (1) : . [pub] 20201231

Language English Country Switzerland

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc21019609

Grant support
16-30544a the Grant Agency of the Ministry of Health
IN00023001 MH CR-DRO
18-07983S the Czech Science Foundation
282216 the Charles University Grant Agency

Early detection of metastasis is crucial for successful cancer treatment. Sentinel lymph node (SLN) biopsies are used to detect possible pathways of metastasis spread. We present a unique non-invasive diagnostic alternative to biopsy along with an intraoperative imaging tool for surgery proven on an in vivo animal tumor model. Our approach is based on mannan-based copolymers synergistically targeting: (1) SLNs and macrophage-infiltrated solid tumor areas via the high-affinity DC-SIGN (dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin) receptors and (2) tumors via the enhanced permeability and retention (EPR) effect. The polymer conjugates were modified with the imaging probes for visualization with magnetic resonance (MR) and fluorescence imaging, respectively, and with poly(2-methyl-2-oxazoline) (POX) to lower unwanted accumulation in internal organs and to slow down the biodegradation rate. We demonstrated that these polymer conjugates were successfully accumulated in tumors, SLNs and other lymph nodes. Modification with POX resulted in lower accumulation not only in internal organs, but also in lymph nodes and tumors. Importantly, we have shown that mannan-based polymer carriers are non-toxic and, when applied to an in vivo murine cancer model, and offer promising potential as the versatile imaging agents.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21019609
003      
CZ-PrNML
005      
20210830101207.0
007      
ta
008      
210728s2020 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/molecules26010146 $2 doi
035    __
$a (PubMed)33396204
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Jirátová, Markéta $u Institute for Clinical and Experimental Medicine (IKEM), 140 21 Prague, Czech Republic $u Department of Physiology, Faculty of Science, Charles University, 128 00 Prague, Czech Republic
245    10
$a Mannan-Based Nanodiagnostic Agents for Targeting Sentinel Lymph Nodes and Tumors / $c M. Jirátová, A. Gálisová, M. Rabyk, E. Sticová, M. Hrubý, D. Jirák
520    9_
$a Early detection of metastasis is crucial for successful cancer treatment. Sentinel lymph node (SLN) biopsies are used to detect possible pathways of metastasis spread. We present a unique non-invasive diagnostic alternative to biopsy along with an intraoperative imaging tool for surgery proven on an in vivo animal tumor model. Our approach is based on mannan-based copolymers synergistically targeting: (1) SLNs and macrophage-infiltrated solid tumor areas via the high-affinity DC-SIGN (dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin) receptors and (2) tumors via the enhanced permeability and retention (EPR) effect. The polymer conjugates were modified with the imaging probes for visualization with magnetic resonance (MR) and fluorescence imaging, respectively, and with poly(2-methyl-2-oxazoline) (POX) to lower unwanted accumulation in internal organs and to slow down the biodegradation rate. We demonstrated that these polymer conjugates were successfully accumulated in tumors, SLNs and other lymph nodes. Modification with POX resulted in lower accumulation not only in internal organs, but also in lymph nodes and tumors. Importantly, we have shown that mannan-based polymer carriers are non-toxic and, when applied to an in vivo murine cancer model, and offer promising potential as the versatile imaging agents.
650    _2
$a zvířata $7 D000818
650    _2
$a apoptóza $7 D017209
650    _2
$a nádory prsu $x metabolismus $x patologie $7 D001943
650    _2
$a proliferace buněk $7 D049109
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a lidé $7 D006801
650    _2
$a lymfatické metastázy $7 D008207
650    _2
$a mannany $x chemie $x metabolismus $7 D008351
650    _2
$a myši $7 D051379
650    _2
$a myši inbrední BALB C $7 D008807
650    _2
$a myši inbrední C3H $7 D008809
650    _2
$a myši nahé $7 D008819
650    _2
$a nanočástice $x aplikace a dávkování $x chemie $7 D053758
650    _2
$a optické zobrazování $7 D061848
650    _2
$a sentinelová uzlina $x metabolismus $x patologie $7 D000071036
650    _2
$a nádorové buňky kultivované $7 D014407
650    _2
$a xenogenní modely - testy antitumorózní aktivity $7 D023041
655    _2
$a časopisecké články $7 D016428
700    1_
$a Gálisová, Andrea $u Institute for Clinical and Experimental Medicine (IKEM), 140 21 Prague, Czech Republic
700    1_
$a Rabyk, Maria $u Institute of Macromolecular Chemistry, Czech Academy of Sciences, 162 00 Prague, Czech Republic
700    1_
$a Sticová, Eva $u Institute for Clinical and Experimental Medicine (IKEM), 140 21 Prague, Czech Republic
700    1_
$a Hrubý, Martin $u Institute of Macromolecular Chemistry, Czech Academy of Sciences, 162 00 Prague, Czech Republic
700    1_
$a Jirák, Daniel $u Institute for Clinical and Experimental Medicine (IKEM), 140 21 Prague, Czech Republic $u Institute of Biophysics and Informatics, 1st Faculty of Medicine, Charles University, 121 08 Prague, Czech Republic
773    0_
$w MED00180394 $t Molecules (Basel, Switzerland) $x 1420-3049 $g Roč. 26, č. 1 (2020)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/33396204 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20210728 $b ABA008
991    __
$a 20210830101207 $b ABA008
999    __
$a ok $b bmc $g 1690435 $s 1140055
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 26 $c 1 $e 20201231 $i 1420-3049 $m Molecules $n Molecules $x MED00180394
GRA    __
$a 16-30544a $p the Grant Agency of the Ministry of Health
GRA    __
$a IN00023001 $p MH CR-DRO
GRA    __
$a 18-07983S $p the Czech Science Foundation
GRA    __
$a 282216 $p the Charles University Grant Agency
LZP    __
$a Pubmed-20210728
Back

Find record

Citation metrics

Archiving options