• Something wrong with this record ?

Prdm9 Intersubspecific Interactions in Hybrid Male Sterility of House Mouse

A. Mukaj, J. Piálek, V. Fotopulosova, AP. Morgan, L. Odenthal-Hesse, ED. Parvanov, J. Forejt

. 2020 ; 37 (12) : 3423-3438. [pub] 20201216

Language English Country United States

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc21019638

The classical definition posits hybrid sterility as a phenomenon when two parental taxa each of which is fertile produce a hybrid that is sterile. The first hybrid sterility gene in vertebrates, Prdm9, coding for a histone methyltransferase, was identified in crosses between two laboratory mouse strains derived from Mus mus musculus and M. m. domesticus subspecies. The unique function of PRDM9 protein in the initiation of meiotic recombination led to the discovery of the basic molecular mechanism of hybrid sterility in laboratory crosses. However, the role of this protein as a component of reproductive barrier outside the laboratory model remained unclear. Here, we show that the Prdm9 allelic incompatibilities represent the primary cause of reduced fertility in intersubspecific hybrids between M. m. musculus and M. m. domesticus including 16 musculus and domesticus wild-derived strains. Disruption of fertility phenotypes correlated with the rate of failure of synapsis between homologous chromosomes in meiosis I and with early meiotic arrest. All phenotypes were restored to normal when the domesticus Prdm9dom2 allele was substituted with the Prdm9dom2H humanized variant. To conclude, our data show for the first time the male infertility of wild-derived musculus and domesticus subspecies F1 hybrids controlled by Prdm9 as the major hybrid sterility gene. The impairment of fertility surrogates, testes weight and sperm count, correlated with increasing difficulties of meiotic synapsis of homologous chromosomes and with meiotic arrest, which we suppose reflect the increasing asymmetry of PRDM9-dependent DNA double-strand breaks.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21019638
003      
CZ-PrNML
005      
20210830101225.0
007      
ta
008      
210728s2020 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1093/molbev/msaa167 $2 doi
035    __
$a (PubMed)32642764
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Mukaj, Amisa $u Department of Mouse Molecular Genetics, Institute of Molecular Genetics of the Czech Academy of Science, Vestec, Czech Republic
245    10
$a Prdm9 Intersubspecific Interactions in Hybrid Male Sterility of House Mouse / $c A. Mukaj, J. Piálek, V. Fotopulosova, AP. Morgan, L. Odenthal-Hesse, ED. Parvanov, J. Forejt
520    9_
$a The classical definition posits hybrid sterility as a phenomenon when two parental taxa each of which is fertile produce a hybrid that is sterile. The first hybrid sterility gene in vertebrates, Prdm9, coding for a histone methyltransferase, was identified in crosses between two laboratory mouse strains derived from Mus mus musculus and M. m. domesticus subspecies. The unique function of PRDM9 protein in the initiation of meiotic recombination led to the discovery of the basic molecular mechanism of hybrid sterility in laboratory crosses. However, the role of this protein as a component of reproductive barrier outside the laboratory model remained unclear. Here, we show that the Prdm9 allelic incompatibilities represent the primary cause of reduced fertility in intersubspecific hybrids between M. m. musculus and M. m. domesticus including 16 musculus and domesticus wild-derived strains. Disruption of fertility phenotypes correlated with the rate of failure of synapsis between homologous chromosomes in meiosis I and with early meiotic arrest. All phenotypes were restored to normal when the domesticus Prdm9dom2 allele was substituted with the Prdm9dom2H humanized variant. To conclude, our data show for the first time the male infertility of wild-derived musculus and domesticus subspecies F1 hybrids controlled by Prdm9 as the major hybrid sterility gene. The impairment of fertility surrogates, testes weight and sperm count, correlated with increasing difficulties of meiotic synapsis of homologous chromosomes and with meiotic arrest, which we suppose reflect the increasing asymmetry of PRDM9-dependent DNA double-strand breaks.
650    _2
$a zvířata $7 D000818
650    _2
$a ženské pohlaví $7 D005260
650    12
$a genová introgrese $7 D000079425
650    _2
$a histonlysin-N-methyltransferasa $x genetika $7 D011495
650    _2
$a infertilita $x genetika $7 D007246
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a meióza $7 D008540
650    _2
$a myši $x genetika $7 D051379
650    _2
$a fylogeografie $7 D058974
650    12
$a reprodukční izolace $7 D060047
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Piálek, Jaroslav $u Research Facility Studenec, Institute of Vertebrate Biology of the Czech Academy of Sciences, Brno, Czech Republic
700    1_
$a Fotopulosova, Vladana $u Department of Mouse Molecular Genetics, Institute of Molecular Genetics of the Czech Academy of Science, Vestec, Czech Republic
700    1_
$a Morgan, Andrew Parker $u Department of Pediatrics, Duke University Hospital, Durham, NC
700    1_
$a Odenthal-Hesse, Linda $u Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Ploen, Germany
700    1_
$a Parvanov, Emil D $u Department of Mouse Molecular Genetics, Institute of Molecular Genetics of the Czech Academy of Science, Vestec, Czech Republic
700    1_
$a Forejt, Jiri $u Department of Mouse Molecular Genetics, Institute of Molecular Genetics of the Czech Academy of Science, Vestec, Czech Republic
773    0_
$w MED00006601 $t Molecular biology and evolution $x 1537-1719 $g Roč. 37, č. 12 (2020), s. 3423-3438
856    41
$u https://pubmed.ncbi.nlm.nih.gov/32642764 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20210728 $b ABA008
991    __
$a 20210830101225 $b ABA008
999    __
$a ok $b bmc $g 1690453 $s 1140084
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 37 $c 12 $d 3423-3438 $e 20201216 $i 1537-1719 $m Molecular biology and evolution $n Mol Biol Evol $x MED00006601
LZP    __
$a Pubmed-20210728

Find record

Citation metrics

Archiving options