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Příručka odpovídá na časté otázky, které se týkají příznaků a léčby endometriózy a neplodnosti žen. Určeno široké veřejnosti, ženám.
- MeSH
- endometrióza * diagnóza terapie MeSH
- fyzioterapie (techniky) MeSH
- ženská infertilita diagnóza terapie MeSH
- Check Tag
- ženské pohlaví MeSH
- Publikační typ
- populární práce MeSH
- příručky MeSH
- Konspekt
- Gynekologie. Porodnictví
- NLK Obory
- gynekologie a porodnictví
- zdravotní výchova
- NLK Publikační typ
- otázky a odpovědi
PRDM9-mediated reproductive isolation was first described in the progeny of Mus musculus musculus (MUS) PWD/Ph and Mus musculus domesticus (DOM) C57BL/6J inbred strains. These male F1 hybrids fail to complete chromosome synapsis and arrest meiosis at prophase I, due to incompatibilities between the Prdm9 gene and hybrid sterility locus Hstx2. We identified 14 alleles of Prdm9 in exon 12, encoding the DNA-binding domain of the PRDM9 protein in outcrossed wild mouse populations from Europe, Asia, and the Middle East, 8 of which are novel. The same allele was found in all mice bearing introgressed t-haplotypes encompassing Prdm9. We asked whether 7 novel Prdm9 alleles in MUS populations and the t-haplotype allele in 1 MUS and 3 DOM populations induce Prdm9-mediated reproductive isolation. The results show that only combinations of the dom2 allele of DOM origin and the MUS msc1 allele ensure complete infertility of intersubspecific hybrids in outcrossed wild populations and inbred mouse strains examined so far. The results further indicate that MUS mice may share the erasure of PRDM9msc1 binding motifs in populations with different Prdm9 alleles, which implies that erased PRDM9 binding motifs may be uncoupled from their corresponding Prdm9 alleles at the population level. Our data corroborate the model of Prdm9-mediated hybrid sterility beyond inbred strains of mice and suggest that sterility alleles of Prdm9 may be rare.
The quandary known as the Intracytoplasmic Sperm Injection (ICSI) paradox is found at the juncture of Assisted Reproductive Technology (ART) and 'andrological ignorance' - a term coined to denote the undervalued treatment and comprehension of male infertility. The prevalent use of ICSI as a solution for severe male infertility, despite its potential to propagate genetically defective sperm, consequently posing a threat to progeny health, illuminates this paradox. We posit that the meteoric rise in Industrial Revolution 4.0 (IR 4.0) and Artificial Intelligence (AI) technologies holds the potential for a transformative shift in addressing male infertility, specifically by mitigating the limitations engendered by 'andrological ignorance.' We advocate for the urgent need to transcend andrological ignorance, envisaging AI as a cornerstone in the precise diagnosis and treatment of the root causes of male infertility. This approach also incorporates the identification of potential genetic defects in descendants, the establishment of knowledge platforms dedicated to male reproductive health, and the optimization of therapeutic outcomes. Our hypothesis suggests that the assimilation of AI could streamline ICSI implementation, leading to an overall enhancement in the realm of male fertility treatments. However, it is essential to conduct further investigations to substantiate the efficacy of AI applications in a clinical setting. This article emphasizes the significance of harnessing AI technologies to optimize patient outcomes in the fast-paced domain of reproductive medicine, thereby fostering the well-being of upcoming generations.
- MeSH
- asistovaná reprodukce MeSH
- intracytoplazmatické injekce spermie * MeSH
- lidé MeSH
- mužská infertilita * diagnóza genetika terapie MeSH
- sperma MeSH
- umělá inteligence MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- dopisy MeSH
Asistovaná reprodukcia je pomerne rýchlo sa rozvíjajúci odbor medicíny, ktorý poskytuje liečbu neplodným párom. Od narodenia prvého dieťaťa zo „skúmavky“ uplynulo viac než 45 rokov, za ktoré obdobie boli vyvinuté rôzne metodiky za účelom zvýšenia úspešnosti liečby. Samotná spontánna koncepcia je podmienená mnohými faktormi, o ktorých máme aj v súčasnosti pomerne málo informácií. Vývoj asistovanej reprodukcie postupne odhaľuje jednotlivé významné procesy a stavy ktoré ovplyvňujú vznik a vývoj úspešnej tehotnosti. Diabetes mellitus a s ním spojené metabolické poruchy majú negatívny vplyv na kvalitu pohlavných buniek, fertilizáciu, nidáciu a vývoj tehotnosti. Identifikácia a úspešná liečba diabetu má preto dôležitý význam pred zahájením liečby neplodnosti.
Assisted reproduction is a relatively rapidly developing field of medicine focused on the treatment of infertile couples. More than 45 years have passed since the birth of the first “test- tube baby”. Since that time various methods were developed to increase the success rate of the treatment. Spontaneous conception itself is conditioned by many factors, about which we still have relatively little information. The development of assisted reproduction techniques gradually reveals important processes and conditions that influence the development of a successful pregnancy. Diabetes mellitus and associated metabolic disorders have a negative impact on the quality of gametes, fertilization as well as the development of pregnancy. Identification and successful treatment of diabetes plays a key role before starting infertility treatment.
- MeSH
- asistovaná reprodukce MeSH
- diabetes mellitus MeSH
- erektilní dysfunkce etiologie terapie MeSH
- fertilizace in vitro metody MeSH
- komplikace diabetu * prevence a kontrola MeSH
- lidé MeSH
- menstruační cyklus MeSH
- mužská infertilita diagnóza etiologie terapie MeSH
- oocyty MeSH
- spermatogeneze MeSH
- syndrom polycystických ovarií diagnóza komplikace terapie MeSH
- ženská infertilita diagnóza etiologie terapie MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
We established efficient first trimester prediction models for small-for-gestational age (SGA) and fetal growth restriction (FGR) without the presence of preeclampsia (PE) regardless of the gestational age of the onset of the disease [early FGR occurring before 32 gestational week or late FGR occurring after 32 gestational week]. The retrospective study was performed on singleton Caucasian pregnancies (n = 6440) during the period 11/2012-3/2020. Finally, 4469 out of 6440 pregnancies had complete medical records since they delivered in the Institute for the Care of Mother and Child, Prague, Czech Republic. The study included all cases diagnosed with SGA (n = 37) or FGR (n = 82) without PE, and 80 selected normal pregnancies. Four microRNAs (miR-1-3p, miR-20a-5p, miR-146a-5p, and miR-181a-5p) identified 75.68 % SGA cases at 10.0 % false positive rate (FPR). Eight microRNAs (miR-1-3p, miR-20a-5p, miR-20b-5p, miR-126-3p, miR-130b-3p, miR-146a-5p, miR-181a-5p, and miR-499a-5p) identified 83.80 % SGA cases at 10.0 % FPR. The prediction model for SGA based on microRNAs was further improved via implementation of maternal clinical characteristics [maternal age and BMI, an infertility treatment by assisted reproductive technology (ART), first trimester screening for PE and/or FGR and for spontaneous preterm, both by FMF algorithm]. Then 81.08 % and 89.19 % pregnancies developing SGA were identified at 10.0 % FPR in case of utilization of 4 microRNA and 8 microRNA biomarkers. Simplified prediction model for SGA based on limited number of maternal clinical characteristics (maternal age and BMI, an infertility treatment by ART, and 4 microRNAs) does not improve the detection rate of SGA (70.27 % SGA cases at 10.0 % FPR) when compared with prediction model for SGA based just on the expression profile of 4 or 8 microRNAs biomarkers. Seven microRNAs only (miR-16-5p, miR-20a-5p, miR-145-5p, miR-146a-5p, miR-181a-5p, miR-342-3p, and miR-574-3p) identified 42.68 % FGR cases at 10.0 % FPR (AUC 0.725). However, the combination of 10 microRNAs only (miR-16-5p, miR-20a-5p, miR-100-5p, miR-143-3p, miR-145-5p, miR-146a-5p, miR-181a-5p, miR-195-5p, miR-342-3p, and miR-574-3p) reached a higher discrimination power (AUC 0.774). It identified 40.24 % FGR cases at 10.0 % FPR. The prediction model for any subtype of FGR based on microRNAs was further improved via implementation of maternal clinical characteristics [maternal age and BMI, an infertility treatment by ART, the parity (nulliparity), the occurrence of SGA or FGR in previous gestation, and the occurrence of any autoimmune disorder, and the presence of chronic hypertension]. Then 64.63 % and 65.85 % pregnancies destinated to develop FGR were identified at 10.0 % FPR in case of utilization of 7 microRNA biomarkers or 10 microRNA biomarkers. When other clinical variables next to those ones mentioned above such as first trimester screening for PE and/or FGR and for spontaneous preterm, both by FMF algorithm, were added to the prediction model for FGR, the detection power was even increased to 74.39 % cases and 78.05 % cases at 10.0 % FPR.
- MeSH
- biologické markery MeSH
- dítě MeSH
- gestační stáří MeSH
- infertilita * MeSH
- kojenec MeSH
- lidé MeSH
- mikro RNA * genetika metabolismus MeSH
- novorozenec MeSH
- plod metabolismus MeSH
- preeklampsie * genetika MeSH
- první trimestr těhotenství MeSH
- retrospektivní studie MeSH
- růstová retardace plodu genetika diagnóza MeSH
- těhotenství MeSH
- Check Tag
- dítě MeSH
- kojenec MeSH
- lidé MeSH
- novorozenec MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- antibakteriální látky terapeutické užití MeSH
- aplikace inhalační MeSH
- cilie patologie MeSH
- dechová cvičení metody MeSH
- diferenciální diagnóza MeSH
- elektronová mikroskopie metody MeSH
- genetické testování metody MeSH
- infekce dýchací soustavy etiologie MeSH
- infertilita etiologie MeSH
- lidé MeSH
- oxid dusnatý analýza MeSH
- poruchy ciliární motility * diagnóza patologie terapie MeSH
- situs inversus etiologie MeSH
- videomikroskopie metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
Asistovaná reprodukce je nedílnou součástí léčby neplodnosti. Základní metodou je mimotělní oplození (IVF – fertilizace in vitro), běžně se používá i intracytoplazmatická injekce spermie do oocytu, kryokonzervace spermií, oocytů a embryí. Podíl embryí preimplantačně geneticky testovaných (PGT) na chromozomální aneuploidie nebo vady konkrétních genů stoupá. Významné je i použití darovaných oocytů, zejména z důvodu vyčerpání ovariální rezervy u žen starších 40 let. Efektivita léčby je vždy velmi zásadně závislá na věku ženy. Léčba asistovanou reprodukci je v Česku velmi dobře dostupná, stejně tak i záchrana plodnosti zmražením spermií/oocytů.
Assisted reproduction is an integral part of infertility treatment. The basic method is in vitro fertilisation (IVF), and intracytoplasmic sperm injection into the oocyte, cryopreservation of sperm, oocytes and embryos is also commonly used. The proportion of embryos tested with preimplantation genetic testing (PGT) for chromosomal aneuploidy or defects in specific genes is increasing. The use of donated oocytes is also significant, particularly because of the depletion of ovarian reserve in women over 40 years of age. The effectiveness of treatment is always very fundamentally dependent on the age of the woman. Assisted reproductive treatment is very well available in the Czech Republic, as is fertility preservation by sperm/oocyte freezing.
- MeSH
- asistovaná reprodukce * ekonomika zákonodárství a právo MeSH
- infertilita terapie MeSH
- lidé MeSH
- preimplantační diagnóza MeSH
- registrace MeSH
- věkové faktory MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- přehledy MeSH
- Geografické názvy
- Česká republika MeSH
Folitropin delta je moderní rekombinantní gonadotropin produkovaný na lidských buněčných liniích PER.C6 vykazující shodný glykosylační profil s lidským folikulostimulačním hormonem v porovnání s rekombinantními gonadotropiny produkovanými na zvířecích buněčných liniích. Cílem tohoto článku je podat shrnutí dosud publikovaných dat.
New recombinant gonadotropin-follitropin delta produced on human cell lines PER.C6 shows the same glycosylation profiles as human follicle stimulating hormone in comparison with recombinant gonadotropins produced on animal cell lines. The aim of the article is to summarize published data.
- MeSH
- infertilita * etiologie prevence a kontrola MeSH
- lidé MeSH
- nemoci dělohy komplikace patologie MeSH
- nemoci ovaria etiologie komplikace MeSH
- nemoci vejcovodů diagnóza komplikace MeSH
- reprodukční zdraví * MeSH
- rizikové faktory MeSH
- spermatogeneze MeSH
- věkové faktory MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
