Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Production of Highly Active Recombinant Dermonecrotic Toxin of Bordetella Pertussis

O. Stanek, I. Linhartova, J. Holubova, L. Bumba, Z. Gardian, A. Malandra, B. Bockova, S. Teruya, Y. Horiguchi, R. Osicka, P. Sebo

. 2020 ; 12 (9) : . [pub] 20200915

Language English Country Switzerland

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc21020126

Grant support
19-27630X Grantová Agentura České Republiky - International
19-12695S Grantová Agentura České Republiky - International
LM2018133 Ministerstvo školství, mládeže a tělovýchovy České republiky - International
LM2015062 Czech-BioImaging Ministerstvo školství, mládeže a tělovýchovy České republiky - International

Pathogenic Bordetella bacteria release a neurotropic dermonecrotic toxin (DNT) that is endocytosed into animal cells and permanently activates the Rho family GTPases by polyamination or deamidation of the glutamine residues in their switch II regions (e.g., Gln63 of RhoA). DNT was found to enable high level colonization of the nasal cavity of pigs by B. bronchiseptica and the capacity of DNT to inhibit differentiation of nasal turbinate bone osteoblasts causes atrophic rhinitis in infected pigs. However, it remains unknown whether DNT plays any role also in virulence of the human pathogen B. pertussis and in pathogenesis of the whooping cough disease. We report a procedure for purification of large amounts of LPS-free recombinant DNT that exhibits a high biological activity on cells expressing the DNT receptors Cav3.1 and Cav3.2. Electron microscopy and single particle image analysis of negatively stained preparations revealed that the DNT molecule adopts a V-shaped structure with well-resolved protein domains. These results open the way to structure-function studies on DNT and its interactions with airway epithelial layers.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21020126
003      
CZ-PrNML
005      
20210830101734.0
007      
ta
008      
210728s2020 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/toxins12090596 $2 doi
035    __
$a (PubMed)32942577
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Stanek, Ondrej $u Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic
245    10
$a Production of Highly Active Recombinant Dermonecrotic Toxin of Bordetella Pertussis / $c O. Stanek, I. Linhartova, J. Holubova, L. Bumba, Z. Gardian, A. Malandra, B. Bockova, S. Teruya, Y. Horiguchi, R. Osicka, P. Sebo
520    9_
$a Pathogenic Bordetella bacteria release a neurotropic dermonecrotic toxin (DNT) that is endocytosed into animal cells and permanently activates the Rho family GTPases by polyamination or deamidation of the glutamine residues in their switch II regions (e.g., Gln63 of RhoA). DNT was found to enable high level colonization of the nasal cavity of pigs by B. bronchiseptica and the capacity of DNT to inhibit differentiation of nasal turbinate bone osteoblasts causes atrophic rhinitis in infected pigs. However, it remains unknown whether DNT plays any role also in virulence of the human pathogen B. pertussis and in pathogenesis of the whooping cough disease. We report a procedure for purification of large amounts of LPS-free recombinant DNT that exhibits a high biological activity on cells expressing the DNT receptors Cav3.1 and Cav3.2. Electron microscopy and single particle image analysis of negatively stained preparations revealed that the DNT molecule adopts a V-shaped structure with well-resolved protein domains. These results open the way to structure-function studies on DNT and its interactions with airway epithelial layers.
650    _2
$a buňky 3T3 $7 D016475
650    _2
$a buňky A549 $7 D000072283
650    _2
$a zvířata $7 D000818
650    _2
$a novorozená zvířata $7 D000831
650    _2
$a Bordetella pertussis $x enzymologie $x genetika $x patogenita $7 D001886
650    _2
$a vápníkové kanály - typ T $x genetika $x metabolismus $7 D020747
650    _2
$a epitelové buňky $x metabolismus $x ultrastruktura $7 D004847
650    _2
$a lidé $7 D006801
650    _2
$a myši $7 D051379
650    _2
$a myši inbrední BALB C $7 D008807
650    _2
$a nekróza $7 D009336
650    _2
$a vazba proteinů $7 D011485
650    _2
$a proteinové domény $7 D000072417
650    _2
$a rekombinantní proteiny $x metabolismus $7 D011994
650    _2
$a kůže $x účinky léků $x patologie $7 D012867
650    _2
$a vztahy mezi strukturou a aktivitou $7 D013329
650    _2
$a transglutaminasy $x genetika $x metabolismus $x toxicita $x ultrastruktura $7 D011503
650    _2
$a faktory virulence rodu Bordetella $x genetika $x metabolismus $x toxicita $7 D010566
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Linhartova, Irena $u Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic
700    1_
$a Holubova, Jana $u Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic
700    1_
$a Bumba, Ladislav $u Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic
700    1_
$a Gardian, Zdenko $u Institute of Parasitology, Biology Centre, The Czech Academy of Sciences, Branišovská 31, 370 05 České Budějovice, Czech Republic
700    1_
$a Malandra, Anna $u Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic
700    1_
$a Bockova, Barbora $u Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic
700    1_
$a Teruya, Shihono $u Department of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan
700    1_
$a Horiguchi, Yasuhiko $u Department of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan
700    1_
$a Osicka, Radim $u Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic
700    1_
$a Sebo, Peter $u Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic
773    0_
$w MED00177194 $t Toxins $x 2072-6651 $g Roč. 12, č. 9 (2020)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/32942577 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20210728 $b ABA008
991    __
$a 20210830101734 $b ABA008
999    __
$a ok $b bmc $g 1690835 $s 1140572
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 12 $c 9 $e 20200915 $i 2072-6651 $m Toxins $n Toxins $x MED00177194
GRA    __
$a 19-27630X $p Grantová Agentura České Republiky $2 International
GRA    __
$a 19-12695S $p Grantová Agentura České Republiky $2 International
GRA    __
$a LM2018133 $p Ministerstvo školství, mládeže a tělovýchovy České republiky $2 International
GRA    __
$a LM2015062 Czech-BioImaging $p Ministerstvo školství, mládeže a tělovýchovy České republiky $2 International
LZP    __
$a Pubmed-20210728

Find record

Citation metrics

Archiving options