-
Something wrong with this record ?
Lactobacilli-dominated cervical microbiota in women with preterm prelabor rupture of membranes
M. Kacerovsky, L. Pliskova, R. Bolehovska, R. Gerychova, P. Janku, P. Matlak, O. Simetka, T. Faist, J. Mls, P. Vescicik, H. Zemlickova, B. Jacobsson, I. Musilova
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
NV16-28587A
MZ0
CEP Register
Digital library NLK
Full text - Article
NLK
Free Medical Journals
from 1967 to 1 year ago
ProQuest Central
from 2016-01-01 to 1 year ago
Health & Medicine (ProQuest)
from 2016-01-01 to 1 year ago
Public Health Database (ProQuest)
from 2016-01-01 to 1 year ago
- MeSH
- Amniocentesis methods MeSH
- Cervix Uteri microbiology MeSH
- Chlamydia trachomatis MeSH
- Chorioamnionitis microbiology MeSH
- Lactobacillus crispatus * MeSH
- Lactobacillus * MeSH
- Humans MeSH
- Microbiota MeSH
- Mycoplasma hominis MeSH
- Infant, Newborn MeSH
- Amniotic Fluid microbiology MeSH
- Obstetric Labor, Premature MeSH
- Fetal Membranes, Premature Rupture microbiology MeSH
- Retrospective Studies MeSH
- Pregnancy MeSH
- Ureaplasma MeSH
- Check Tag
- Humans MeSH
- Infant, Newborn MeSH
- Pregnancy MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
BACKGROUND: To determine the association between microbial invasion of the amniotic cavity (MIAC) and the presence of Lactobacillus crispatus- or Lactobacillus iners-dominated cervical microbiota in pregnancies with preterm prelabor rupture of membrane. Next, to assess the relationship between the presence of L. crispatus- or L. iners-dominated cervical microbiota and short-term neonatal morbidity. METHOD: A total of 311 women were included. Cervical samples were obtained using a Dacron polyester swab and amniotic fluid samples were obtained by transabdominal amniocentesis. Bacterial DNA, L. crispatus, and L. iners in the cervical samples were assessed by PCR. Cervical microbiota was assigned as L. crispatus- or L. iners-dominated when the relative abundance of L. crispatus or L. iners was ≥50% of the whole cervical microbiota, respectively. RESULTS: Women with MIAC showed a lower rate of L. crispatus-dominated cervical microbiota (21% vs. 39%; p = 0.003) than those without MIAC. Lactobacillus crispatus-dominated cervical microbiota was associated with a lower rate of early-onset sepsis (0% vs. 5%; p = 0.02). CONCLUSIONS: The presence of L. crispatus-dominated cervical microbiota in women with preterm prelabor rupture of membrane was associated with a lower risk of intra-amniotic complications and subsequent development of early-onset sepsis of newborns.
Biomedical Research Center University Hospital Hradec Kralove Hradec Kralove Czech Republic
Department of Obstetrics and Gynecology University Hospital Ostrava Ostrava Czech Republic
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc21020667
- 003
- CZ-PrNML
- 005
- 20210830102258.0
- 007
- ta
- 008
- 210728s2020 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1038/s41390-019-0692-1 $2 doi
- 035 __
- $a (PubMed)31791041
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Kacerovsky, Marian $u Department of Obstetrics and Gynecology, University Hospital Hradec Kralove, Charles University Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic. kacermar@fnhk.cz $u Biomedical Research Center, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic. kacermar@fnhk.cz
- 245 10
- $a Lactobacilli-dominated cervical microbiota in women with preterm prelabor rupture of membranes / $c M. Kacerovsky, L. Pliskova, R. Bolehovska, R. Gerychova, P. Janku, P. Matlak, O. Simetka, T. Faist, J. Mls, P. Vescicik, H. Zemlickova, B. Jacobsson, I. Musilova
- 520 9_
- $a BACKGROUND: To determine the association between microbial invasion of the amniotic cavity (MIAC) and the presence of Lactobacillus crispatus- or Lactobacillus iners-dominated cervical microbiota in pregnancies with preterm prelabor rupture of membrane. Next, to assess the relationship between the presence of L. crispatus- or L. iners-dominated cervical microbiota and short-term neonatal morbidity. METHOD: A total of 311 women were included. Cervical samples were obtained using a Dacron polyester swab and amniotic fluid samples were obtained by transabdominal amniocentesis. Bacterial DNA, L. crispatus, and L. iners in the cervical samples were assessed by PCR. Cervical microbiota was assigned as L. crispatus- or L. iners-dominated when the relative abundance of L. crispatus or L. iners was ≥50% of the whole cervical microbiota, respectively. RESULTS: Women with MIAC showed a lower rate of L. crispatus-dominated cervical microbiota (21% vs. 39%; p = 0.003) than those without MIAC. Lactobacillus crispatus-dominated cervical microbiota was associated with a lower rate of early-onset sepsis (0% vs. 5%; p = 0.02). CONCLUSIONS: The presence of L. crispatus-dominated cervical microbiota in women with preterm prelabor rupture of membrane was associated with a lower risk of intra-amniotic complications and subsequent development of early-onset sepsis of newborns.
- 650 _2
- $a amniocentéza $x metody $7 D000649
- 650 _2
- $a plodová voda $x mikrobiologie $7 D000653
- 650 _2
- $a cervix uteri $x mikrobiologie $7 D002584
- 650 _2
- $a Chlamydia trachomatis $7 D002692
- 650 _2
- $a chorioamnionitida $x mikrobiologie $7 D002821
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a předčasný odtok plodové vody $x mikrobiologie $7 D005322
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a novorozenec $7 D007231
- 650 12
- $a Lactobacillus $7 D007778
- 650 12
- $a Lactobacillus crispatus $7 D000070003
- 650 _2
- $a mikrobiota $7 D064307
- 650 _2
- $a Mycoplasma hominis $7 D019535
- 650 _2
- $a předčasná porodní činnost $7 D007752
- 650 _2
- $a těhotenství $7 D011247
- 650 _2
- $a retrospektivní studie $7 D012189
- 650 _2
- $a Ureaplasma $7 D014509
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Pliskova, Lenka $u Institute of Clinical Biochemistry and Diagnostics, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic
- 700 1_
- $a Bolehovska, Radka $u Institute of Clinical Biochemistry and Diagnostics, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic
- 700 1_
- $a Gerychova, Romana $u Department of Obstetrics and Gynecology, University Hospital Brno, Faculty of Medicine Masaryk University, Brno, Czech Republic
- 700 1_
- $a Janku, Petr $u Department of Obstetrics and Gynecology, University Hospital Brno, Faculty of Medicine Masaryk University, Brno, Czech Republic
- 700 1_
- $a Matlak, Petr $u Department of Obstetrics and Gynecology, University Hospital Ostrava, Ostrava, Czech Republic
- 700 1_
- $a Simetka, Ondrej $u Department of Obstetrics and Gynecology, University Hospital Ostrava, Ostrava, Czech Republic
- 700 1_
- $a Faist, Tomas $u Department of Obstetrics and Gynecology, University Hospital Hradec Kralove, Charles University Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic
- 700 1_
- $a Mls, Jan $u Department of Obstetrics and Gynecology, University Hospital Hradec Kralove, Charles University Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic
- 700 1_
- $a Vescicik, Petr $u Department of Obstetrics and Gynecology, University Hospital Hradec Kralove, Charles University Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic
- 700 1_
- $a Zemlickova, Helena $u Institute of Clinical Microbiology, University Hospital in Hradec Kralove, Faculty of Medicine Hradec Kralove, Charles University, Hradec Kralove, Czech Republic
- 700 1_
- $a Jacobsson, Bo $u Department of Obstetrics and Gynecology, Institute of Clinical Science, Sahlgrenska Academy, Gothenburg University, Gothenburg, Sweden $u Department of Obstetrics and Gynecology, Region Västra Götaland, Sahlgrenska University Hospital, Gothenburg, Sweden $u Department of Genetics and Bioinformatics, Domain of Health Data and Digitalisation, Institute of Public Health, Oslo, Norway
- 700 1_
- $a Musilova, Ivana $u Department of Obstetrics and Gynecology, University Hospital Hradec Kralove, Charles University Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic
- 773 0_
- $w MED00003741 $t Pediatric research $x 1530-0447 $g Roč. 87, č. 5 (2020), s. 952-960
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/31791041 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20210728 $b ABA008
- 991 __
- $a 20210830102258 $b ABA008
- 999 __
- $a ok $b bmc $g 1691275 $s 1141113
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2020 $b 87 $c 5 $d 952-960 $e 20191202 $i 1530-0447 $m Pediatric research $n Pediatr Res $x MED00003741
- GRA __
- $a NV16-28587A $p MZ0
- LZP __
- $a Pubmed-20210728
