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Primary and secondary anti-viral response captured by the dynamics and phenotype of individual T cell clones
AA. Minervina, MV. Pogorelyy, EA. Komech, VK. Karnaukhov, P. Bacher, E. Rosati, A. Franke, DM. Chudakov, IZ. Mamedov, YB. Lebedev, T. Mora, AM. Walczak
Jazyk angličtina Země Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
24208
European Research Council - International
15-15-00178
Russian Science Foundation
18-29-09132
Russian Foundation for Basic Research
19-54-12011
Russian Foundation for Basic Research
Exc2167
Deutsche Forschungsgemeinschaft
4096610003
Deutsche Forschungsgemeinschaft
075-15-2019-1789
Ministry of Science and Higher Education of the Russian Federation
075-15-2019-1660
Ministry of Science and Higher Education of the Russian Federation
Free Medical Journals od 2012
PubMed Central od 2012
Europe PubMed Central od 2012
ProQuest Central od 2012-01-01
Open Access Digital Library od 2012-01-01
Open Access Digital Library od 2013-01-01
Health & Medicine (ProQuest) od 2012-01-01
ROAD: Directory of Open Access Scholarly Resources od 2012
Odkazy
PubMed
32081129
DOI
10.7554/elife.53704
Knihovny.cz E-zdroje
- MeSH
- časové faktory MeSH
- dospělí MeSH
- epitopy imunologie MeSH
- fenotyp MeSH
- imunologická paměť MeSH
- lidé MeSH
- podskupiny lymfocytů imunologie fyziologie MeSH
- receptory antigenů T-buněk genetika imunologie fyziologie MeSH
- T-lymfocyty imunologie fyziologie virologie MeSH
- transkriptom MeSH
- vakcína proti žluté zimnici imunologie farmakologie MeSH
- virus žluté zimnice imunologie MeSH
- vysoce účinné nukleotidové sekvenování MeSH
- žlutá zimnice imunologie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The diverse repertoire of T-cell receptors (TCR) plays a key role in the adaptive immune response to infections. Using TCR alpha and beta repertoire sequencing for T-cell subsets, as well as single-cell RNAseq and TCRseq, we track the concentrations and phenotypes of individual T-cell clones in response to primary and secondary yellow fever immunization - the model for acute infection in humans - showing their large diversity. We confirm the secondary response is an order of magnitude weaker, albeit ∼10 days faster than the primary one. Estimating the fraction of the T-cell response directed against the single immunodominant epitope, we identify the sequence features of TCRs that define the high precursor frequency of the two major TCR motifs specific for this particular epitope. We also show the consistency of clonal expansion dynamics between bulk alpha and beta repertoires, using a new methodology to reconstruct alpha-beta pairings from clonal trajectories.
Center of Life Sciences Skoltech Moscow Russian Federation
Institute of Clinical Molecular Biology Kiel University Kiel Germany
Institute of Immunology Kiel University Kiel Germany
Masaryk University Central European Institute of Technology Brno Czech Republic
Moscow State University Moscow Russian Federation
Shemyakin Ovchinnikov Institute of Bioorganic Chemistry Moscow Russian Federation
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