Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

The Effect of Resorcinol on Bovine Spermatozoa Parameters in Vitro

M. Massányi, M. Halo, L. Strapáková, T. Slanina, P. Ivanič, E. Strapáková, P. Strapák, M. Halo, A. Greń, G. Formicki, P. Massányi

. 2020 ; 69 (4) : 675-686. [pub] 20200625

Language English Country Czech Republic

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc21021219

The goal of this study was to observe the effect of resorcinol on motility, viability and morphology of bovine spermatozoa. The semen was used from six randomly chosen breeding bulls. Ejaculate was diluted by different solutions of resorcinol in 1:40 ratio. Samples were divided into 7 groups with different concentrations of resorcinol (Control, RES1 - 4 mg/ml, RES2 - 2 mg/ml, RES3 - 1 mg/ml, RES4 - 0.5 mg/ml, RES5 - 0.25 mg/ml and RES6 - 0.125 mg/ml). Motility of spermatozoa was detected using CASA method at temperature of 37 °C in time periods 0, 1, 2, 3, 4 hours from the start of the experiment. Significant motility differences between all groups except control and RES6 with difference of 5.58 %, as well as between RES1 and RES2 groups with difference of 2.17 % were found. Progressive motility had the same significant differences. Spermatozoa viability (MTT test) decreased compared to control in all experimental groups during the entire duration of experiment. Observing morphologically changed spermatozoa, no significant changes were observed and a higher percentage of spermatozoa with separated flagellum in all experimental resorcinol groups compared to control were detected. Also, increased number of spermatozoa with broken flagellum, acrosomal changes and other morphological forms in the group with the highest concentration of resorcinol (RES1) were found. Results of our study clearly show negative effects on motility parameters of spermatozoa which depend on concentration, cultivation temperature and time period.

References provided by Crossref.org

Bibliography, etc.

Literatura

000      
00000naa a2200000 a 4500
001      
bmc21021219
003      
CZ-PrNML
005      
20210924100018.0
007      
ta
008      
210806s2020 xr d f 000 0|eng||
009      
AR
024    7_
$a 10.33549/physiolres.934466 $2 doi
035    __
$a (PubMed)32584138
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Massányi, Martin $7 xx0264292 $u Department of Animal Husbandry, Faculty of Agrobiology and Food Sources, Slovak University of Agriculture, Nitra, Slovak Republic
245    14
$a The Effect of Resorcinol on Bovine Spermatozoa Parameters in Vitro / $c M. Massányi, M. Halo, L. Strapáková, T. Slanina, P. Ivanič, E. Strapáková, P. Strapák, M. Halo, A. Greń, G. Formicki, P. Massányi
504    __
$a Literatura
520    9_
$a The goal of this study was to observe the effect of resorcinol on motility, viability and morphology of bovine spermatozoa. The semen was used from six randomly chosen breeding bulls. Ejaculate was diluted by different solutions of resorcinol in 1:40 ratio. Samples were divided into 7 groups with different concentrations of resorcinol (Control, RES1 - 4 mg/ml, RES2 - 2 mg/ml, RES3 - 1 mg/ml, RES4 - 0.5 mg/ml, RES5 - 0.25 mg/ml and RES6 - 0.125 mg/ml). Motility of spermatozoa was detected using CASA method at temperature of 37 °C in time periods 0, 1, 2, 3, 4 hours from the start of the experiment. Significant motility differences between all groups except control and RES6 with difference of 5.58 %, as well as between RES1 and RES2 groups with difference of 2.17 % were found. Progressive motility had the same significant differences. Spermatozoa viability (MTT test) decreased compared to control in all experimental groups during the entire duration of experiment. Observing morphologically changed spermatozoa, no significant changes were observed and a higher percentage of spermatozoa with separated flagellum in all experimental resorcinol groups compared to control were detected. Also, increased number of spermatozoa with broken flagellum, acrosomal changes and other morphological forms in the group with the highest concentration of resorcinol (RES1) were found. Results of our study clearly show negative effects on motility parameters of spermatozoa which depend on concentration, cultivation temperature and time period.
650    _2
$a zvířata $7 D000818
650    _2
$a skot $7 D002417
650    _2
$a vztah mezi dávkou a účinkem léčiva $7 D004305
650    _2
$a techniky in vitro $7 D066298
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a resorcinoly $x farmakologie $7 D012118
650    _2
$a sperma $x účinky léků $x fyziologie $7 D012661
650    _2
$a motilita spermií $x účinky léků $7 D013081
650    _2
$a spermie $x účinky léků $7 D013094
655    _2
$a časopisecké články $7 D016428
700    1_
$a Strapáková, Lucia $7 xx0264294 $u Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Slovak Republic
700    1_
$a Slanina, Tomáš, $d 1986- $7 xx0249937 $u Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Slovak Republic
700    1_
$a Ivanič, Peter $7 xx0264296 $u Slovak Biological Services, Banská Bystrica, Slovak Republic
700    1_
$a Strapáková, Eva $7 xx0264299 $u Department of Animal Genetics and Breeding Biology, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture, Nitra, Slovak Republic
700    1_
$a Strapák, Peter, $d 1965- $7 jo2016927177 $u Department of Animal Genetics and Breeding Biology, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture, Nitra, Slovak Republic
700    1_
$a Halo, Marko, $d 1967- $7 jcu2012738896 $u Department of Animal Husbandry, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture, Nitra, Slovak Republic
700    1_
$a Greń, A. $u Department of Animal Physiology, Faculty of Exact and Natural Sciences, Pedagogical University of Kraków, Poland
700    1_
$a Formicki, G. $u Department of Animal Physiology, Faculty of Exact and Natural Sciences, Pedagogical University of Kraków, Poland
700    1_
$a Massanyi, Peter, $d 1968- $7 uzp2012691017 $u Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Slovak Republic
773    0_
$w MED00003824 $t Physiological research $x 1802-9973 $g Roč. 69, č. 4 (2020), s. 675-686
856    41
$u https://pubmed.ncbi.nlm.nih.gov/32584138 $y Pubmed
910    __
$a ABA008 $b A 4120 $c 266 $y p $z 0
990    __
$a 20210806 $b ABA008
991    __
$a 20210924100016 $b ABA008
999    __
$a ok $b bmc $g 1697024 $s 1141672
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 69 $c 4 $d 675-686 $e 20200625 $i 1802-9973 $m Physiological research $n Physiol. Res. (Print) $x MED00003824
LZP    __
$b NLK118 $a Pubmed-20210806

Find record

Citation metrics

Archiving options