• Je něco špatně v tomto záznamu ?

Small RNA Sequencing Identifies PIWI-Interacting RNAs Deregulated in Glioblastoma-piR-9491 and piR-12488 Reduce Tumor Cell Colonies In Vitro

M. Bartos, F. Siegl, A. Kopkova, L. Radova, J. Oppelt, M. Vecera, T. Kazda, R. Jancalek, M. Hendrych, M. Hermanova, P. Kasparova, Z. Pleskacova, V. Vybihal, P. Fadrus, M. Smrcka, R. Lakomy, R. Lipina, T. Cesak, O. Slaby, J. Sana

. 2021 ; 11 (-) : 707017. [pub] 20210813

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc21024159

Glioblastoma (GBM) is the most frequently occurring primary malignant brain tumor of astrocytic origin. To change poor prognosis, it is necessary to deeply understand the molecular mechanisms of gliomagenesis and identify new potential biomarkers and therapeutic targets. PIWI-interacting RNAs (piRNAs) help in maintaining genome stability, and their deregulation has already been observed in many tumors. Recent studies suggest that these molecules could also play an important role in the glioma biology. To determine GBM-associated piRNAs, we performed small RNA sequencing analysis in the discovery set of 19 GBM and 11 non-tumor brain samples followed by TaqMan qRT-PCR analyses in the independent set of 77 GBM and 23 non-tumor patients. Obtained data were subsequently bioinformatically analyzed. Small RNA sequencing revealed 58 significantly deregulated piRNA molecules in GBM samples in comparison with non-tumor brain tissues. Deregulation of piR-1849, piR-9491, piR-12487, and piR-12488 was successfully confirmed in the independent groups of patients and controls (all p < 0.0001), and piR-9491 and piR-12488 reduced GBM cells' ability to form colonies in vitro. In addition, piR-23231 was significantly associated with the overall survival of the GBM patients treated with Stupp regimen (p = 0.007). Our results suggest that piRNAs could be a novel promising diagnostic and prognostic biomarker in GBM potentially playing important roles in gliomagenesis.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21024159
003      
CZ-PrNML
005      
20211013134033.0
007      
ta
008      
211006s2021 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3389/fonc.2021.707017 $2 doi
035    __
$a (PubMed)34485142
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Bartos, Michael $u Department of Neurosurgery, University Hospital Hradec Kralove, Hradec Kralove, Czechia
245    10
$a Small RNA Sequencing Identifies PIWI-Interacting RNAs Deregulated in Glioblastoma-piR-9491 and piR-12488 Reduce Tumor Cell Colonies In Vitro / $c M. Bartos, F. Siegl, A. Kopkova, L. Radova, J. Oppelt, M. Vecera, T. Kazda, R. Jancalek, M. Hendrych, M. Hermanova, P. Kasparova, Z. Pleskacova, V. Vybihal, P. Fadrus, M. Smrcka, R. Lakomy, R. Lipina, T. Cesak, O. Slaby, J. Sana
520    9_
$a Glioblastoma (GBM) is the most frequently occurring primary malignant brain tumor of astrocytic origin. To change poor prognosis, it is necessary to deeply understand the molecular mechanisms of gliomagenesis and identify new potential biomarkers and therapeutic targets. PIWI-interacting RNAs (piRNAs) help in maintaining genome stability, and their deregulation has already been observed in many tumors. Recent studies suggest that these molecules could also play an important role in the glioma biology. To determine GBM-associated piRNAs, we performed small RNA sequencing analysis in the discovery set of 19 GBM and 11 non-tumor brain samples followed by TaqMan qRT-PCR analyses in the independent set of 77 GBM and 23 non-tumor patients. Obtained data were subsequently bioinformatically analyzed. Small RNA sequencing revealed 58 significantly deregulated piRNA molecules in GBM samples in comparison with non-tumor brain tissues. Deregulation of piR-1849, piR-9491, piR-12487, and piR-12488 was successfully confirmed in the independent groups of patients and controls (all p < 0.0001), and piR-9491 and piR-12488 reduced GBM cells' ability to form colonies in vitro. In addition, piR-23231 was significantly associated with the overall survival of the GBM patients treated with Stupp regimen (p = 0.007). Our results suggest that piRNAs could be a novel promising diagnostic and prognostic biomarker in GBM potentially playing important roles in gliomagenesis.
655    _2
$a časopisecké články $7 D016428
700    1_
$a Siegl, Frantisek $u Central European Institute of Technology, Masaryk University, Brno, Czechia
700    1_
$a Kopkova, Alena $u Central European Institute of Technology, Masaryk University, Brno, Czechia
700    1_
$a Radova, Lenka $u Central European Institute of Technology, Masaryk University, Brno, Czechia
700    1_
$a Oppelt, Jan $u Central European Institute of Technology, Masaryk University, Brno, Czechia
700    1_
$a Vecera, Marek $u Central European Institute of Technology, Masaryk University, Brno, Czechia
700    1_
$a Kazda, Tomas $u Department of Radiation Oncology, Masaryk Memorial Cancer Institute and Medical Faculty, Masaryk University, Brno, Czechia
700    1_
$a Jancalek, Radim $u Department of Neurosurgery, St. Anne's University Hospital and Medical Faculty, Masaryk University, Brno, Czechia
700    1_
$a Hendrych, Michal $u 1st Department of Pathology, St. Anne's University Hospital and Medical Faculty, Masaryk University, Brno, Czechia
700    1_
$a Hermanova, Marketa $u 1st Department of Pathology, St. Anne's University Hospital and Medical Faculty, Masaryk University, Brno, Czechia
700    1_
$a Kasparova, Petra $u The Fingerland Department of Pathology, University Hospital Hradec Kralove, Hradec Kralove, Czechia
700    1_
$a Pleskacova, Zuzana $u Department of Oncology and Radiotherapy, University Hospital Hradec Kralove, Hradec Kralove, Czechia
700    1_
$a Vybihal, Vaclav $u Department of Neurosurgery, University Hospital Brno, Brno, Czechia
700    1_
$a Fadrus, Pavel $u Department of Neurosurgery, University Hospital Brno, Brno, Czechia
700    1_
$a Smrcka, Martin $u Department of Neurosurgery, University Hospital Brno, Brno, Czechia
700    1_
$a Lakomy, Radek $u Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Brno, Czechia
700    1_
$a Lipina, Radim $u Department of Neurosurgery, University Hospital Ostrava, Ostrava, Czechia
700    1_
$a Cesak, Tomas $u Department of Neurosurgery, University Hospital Hradec Kralove, Hradec Kralove, Czechia
700    1_
$a Slaby, Ondrej $u Central European Institute of Technology, Masaryk University, Brno, Czechia $u Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Brno, Czechia $u Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czechia
700    1_
$a Sana, Jiri $u Central European Institute of Technology, Masaryk University, Brno, Czechia $u Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Brno, Czechia $u Department of Pathology, University Hospital Brno, Brno, Czechia
773    0_
$w MED00182989 $t Frontiers in oncology $x 2234-943X $g Roč. 11, č. - (2021), s. 707017
856    41
$u https://pubmed.ncbi.nlm.nih.gov/34485142 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20211006 $b ABA008
991    __
$a 20211013134030 $b ABA008
999    __
$a ind $b bmc $g 1708208 $s 1144656
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 11 $c - $d 707017 $e 20210813 $i 2234-943X $m Frontiers in oncology $n Front Oncol $x MED00182989
LZP    __
$a Pubmed-20211006

Najít záznam

Citační ukazatele

Možnosti archivace