• Je něco špatně v tomto záznamu ?

Structural basis for +1 ribosomal frameshifting during EF-G-catalyzed translocation

G. Demo, HB. Gamper, AB. Loveland, I. Masuda, CE. Carbone, E. Svidritskiy, YM. Hou, AA. Korostelev

. 2021 ; 12 (1) : 4644. [pub] 20210730

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc21025287

Grantová podpora
R01 AI139202 NIAID NIH HHS - United States
R35 GM127094 NIGMS NIH HHS - United States
R35 GM134931 NIGMS NIH HHS - United States

Frameshifting of mRNA during translation provides a strategy to expand the coding repertoire of cells and viruses. How and where in the elongation cycle +1-frameshifting occurs remains poorly understood. We describe seven ~3.5-Å-resolution cryo-EM structures of 70S ribosome complexes, allowing visualization of elongation and translocation by the GTPase elongation factor G (EF-G). Four structures with a + 1-frameshifting-prone mRNA reveal that frameshifting takes place during translocation of tRNA and mRNA. Prior to EF-G binding, the pre-translocation complex features an in-frame tRNA-mRNA pairing in the A site. In the partially translocated structure with EF-G•GDPCP, the tRNA shifts to the +1-frame near the P site, rendering the freed mRNA base to bulge between the P and E sites and to stack on the 16S rRNA nucleotide G926. The ribosome remains frameshifted in the nearly post-translocation state. Our findings demonstrate that the ribosome and EF-G cooperate to induce +1 frameshifting during tRNA-mRNA translocation.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21025287
003      
CZ-PrNML
005      
20211026133946.0
007      
ta
008      
211013s2021 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1038/s41467-021-24911-1 $2 doi
035    __
$a (PubMed)34330903
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Demo, Gabriel $u RNA Therapeutics Institute, Department of Biochemistry and Molecular Pharmacology, UMass Medical School, Worcester, MA, USA $u Central European Institute of Technology, Masaryk University, Brno, Czech Republic
245    10
$a Structural basis for +1 ribosomal frameshifting during EF-G-catalyzed translocation / $c G. Demo, HB. Gamper, AB. Loveland, I. Masuda, CE. Carbone, E. Svidritskiy, YM. Hou, AA. Korostelev
520    9_
$a Frameshifting of mRNA during translation provides a strategy to expand the coding repertoire of cells and viruses. How and where in the elongation cycle +1-frameshifting occurs remains poorly understood. We describe seven ~3.5-Å-resolution cryo-EM structures of 70S ribosome complexes, allowing visualization of elongation and translocation by the GTPase elongation factor G (EF-G). Four structures with a + 1-frameshifting-prone mRNA reveal that frameshifting takes place during translocation of tRNA and mRNA. Prior to EF-G binding, the pre-translocation complex features an in-frame tRNA-mRNA pairing in the A site. In the partially translocated structure with EF-G•GDPCP, the tRNA shifts to the +1-frame near the P site, rendering the freed mRNA base to bulge between the P and E sites and to stack on the 16S rRNA nucleotide G926. The ribosome remains frameshifted in the nearly post-translocation state. Our findings demonstrate that the ribosome and EF-G cooperate to induce +1 frameshifting during tRNA-mRNA translocation.
650    _2
$a biokatalýza $7 D055162
650    _2
$a elektronová kryomikroskopie $7 D020285
650    _2
$a Escherichia coli $x genetika $x metabolismus $7 D004926
650    _2
$a proteiny z Escherichia coli $x chemie $x genetika $x metabolismus $7 D029968
650    _2
$a posun čtecího rámce na ribozómech $x genetika $7 D018965
650    _2
$a molekulární modely $7 D008958
650    _2
$a konformace nukleové kyseliny $7 D009690
650    _2
$a elongace translace peptidového řetězce $x genetika $7 D010441
650    _2
$a elongační faktor G $x chemie $x genetika $x metabolismus $7 D020653
650    _2
$a konformace proteinů $7 D011487
650    _2
$a messenger RNA $x chemie $x genetika $x metabolismus $7 D012333
650    _2
$a RNA ribozomální 16S $x chemie $x genetika $x metabolismus $7 D012336
650    _2
$a RNA transferová $x chemie $x genetika $x metabolismus $7 D012343
650    _2
$a ribozomy $x genetika $x metabolismus $x ultrastruktura $7 D012270
650    _2
$a tRNA-methyltransferasy $x genetika $x metabolismus $7 D012359
655    _2
$a časopisecké články $7 D016428
655    _2
$a Research Support, N.I.H., Extramural $7 D052061
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Gamper, Howard B $u Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA, USA
700    1_
$a Loveland, Anna B $u RNA Therapeutics Institute, Department of Biochemistry and Molecular Pharmacology, UMass Medical School, Worcester, MA, USA
700    1_
$a Masuda, Isao $u Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA, USA
700    1_
$a Carbone, Christine E $u RNA Therapeutics Institute, Department of Biochemistry and Molecular Pharmacology, UMass Medical School, Worcester, MA, USA
700    1_
$a Svidritskiy, Egor $u RNA Therapeutics Institute, Department of Biochemistry and Molecular Pharmacology, UMass Medical School, Worcester, MA, USA
700    1_
$a Hou, Ya-Ming $u Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA, USA. Ya-Ming.Hou@jefferson.edu
700    1_
$a Korostelev, Andrei A $u RNA Therapeutics Institute, Department of Biochemistry and Molecular Pharmacology, UMass Medical School, Worcester, MA, USA. Andrei.Korostelev@umassmed.edu
773    0_
$w MED00184850 $t Nature communications $x 2041-1723 $g Roč. 12, č. 1 (2021), s. 4644
856    41
$u https://pubmed.ncbi.nlm.nih.gov/34330903 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20211013 $b ABA008
991    __
$a 20211026133952 $b ABA008
999    __
$a ok $b bmc $g 1714372 $s 1145794
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 12 $c 1 $d 4644 $e 20210730 $i 2041-1723 $m Nature communications $n Nat Commun $x MED00184850
GRA    __
$a R01 AI139202 $p NIAID NIH HHS $2 United States
GRA    __
$a R35 GM127094 $p NIGMS NIH HHS $2 United States
GRA    __
$a R35 GM134931 $p NIGMS NIH HHS $2 United States
LZP    __
$a Pubmed-20211013

Najít záznam

Citační ukazatele

Možnosti archivace