-
Je něco špatně v tomto záznamu ?
Phenotypic and Clonal Stability of Antigen-Inexperienced Memory-like T Cells across the Genetic Background, Hygienic Status, and Aging
A. Moudra, V. Niederlova, J. Novotny, L. Schmiedova, J. Kubovciak, T. Matejkova, A. Drobek, M. Pribikova, R. Stopkova, D. Cizkova, A. Neuwirth, J. Michalik, K. Krizova, T. Hudcovic, M. Kolar, H. Kozakova, J. Kreisinger, P. Stopka, O. Stepanek
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
166538
Swiss National Science Foundation - Switzerland
NLK
Free Medical Journals
od 1998 do Před 1 rokem
Freely Accessible Science Journals
od 1998-01-01 do Před 1 rokem
Open Access Digital Library
od 1998-01-01
PubMed
33858960
DOI
10.4049/jimmunol.2001028
Knihovny.cz E-zdroje
- MeSH
- antigeny genetika imunologie MeSH
- fenotyp MeSH
- imunologická paměť genetika imunologie MeSH
- klonální evoluce MeSH
- myši inbrední BALB C MeSH
- myši inbrední C57BL MeSH
- myši transgenní MeSH
- myši MeSH
- nestabilita genomu MeSH
- stárnutí genetika imunologie MeSH
- T-lymfocyty imunologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Ag-inexperienced memory-like T (AIMT) cells are functionally unique T cells, representing one of the two largest subsets of murine CD8+ T cells. However, differences between laboratory inbred strains, insufficient data from germ-free mice, a complete lack of data from feral mice, and an unclear relationship between AIMT cells formation during aging represent major barriers for better understanding of their biology. We performed a thorough characterization of AIMT cells from mice of different genetic background, age, and hygienic status by flow cytometry and multiomics approaches, including analyses of gene expression, TCR repertoire, and microbial colonization. Our data showed that AIMT cells are steadily present in mice, independent of their genetic background and hygienic status. Despite differences in their gene expression profiles, young and aged AIMT cells originate from identical clones. We identified that CD122 discriminates two major subsets of AIMT cells in a strain-independent manner. Whereas thymic CD122LOW AIMT cells (innate memory) prevail only in young animals with high thymic IL-4 production, peripheral CD122HIGH AIMT cells (virtual memory) dominate in aged mice. Cohousing with feral mice changed the bacterial colonization of laboratory strains but had only minimal effects on the CD8+ T cell compartment, including AIMT cells.
Department of Biomedicine University Hospital and University of Basel Basel Switzerland
Department of Zoology Faculty of Science BIOCEV Charles University Vestec Czech Republic
Department of Zoology Faculty of Science Charles University Prague Czech Republic
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc21025798
- 003
- CZ-PrNML
- 005
- 20211208101104.0
- 007
- ta
- 008
- 211013s2021 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.4049/jimmunol.2001028 $2 doi
- 035 __
- $a (PubMed)33858960
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Moudra, Alena $u Laboratory of Adaptive Immunity, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
- 245 10
- $a Phenotypic and Clonal Stability of Antigen-Inexperienced Memory-like T Cells across the Genetic Background, Hygienic Status, and Aging / $c A. Moudra, V. Niederlova, J. Novotny, L. Schmiedova, J. Kubovciak, T. Matejkova, A. Drobek, M. Pribikova, R. Stopkova, D. Cizkova, A. Neuwirth, J. Michalik, K. Krizova, T. Hudcovic, M. Kolar, H. Kozakova, J. Kreisinger, P. Stopka, O. Stepanek
- 520 9_
- $a Ag-inexperienced memory-like T (AIMT) cells are functionally unique T cells, representing one of the two largest subsets of murine CD8+ T cells. However, differences between laboratory inbred strains, insufficient data from germ-free mice, a complete lack of data from feral mice, and an unclear relationship between AIMT cells formation during aging represent major barriers for better understanding of their biology. We performed a thorough characterization of AIMT cells from mice of different genetic background, age, and hygienic status by flow cytometry and multiomics approaches, including analyses of gene expression, TCR repertoire, and microbial colonization. Our data showed that AIMT cells are steadily present in mice, independent of their genetic background and hygienic status. Despite differences in their gene expression profiles, young and aged AIMT cells originate from identical clones. We identified that CD122 discriminates two major subsets of AIMT cells in a strain-independent manner. Whereas thymic CD122LOW AIMT cells (innate memory) prevail only in young animals with high thymic IL-4 production, peripheral CD122HIGH AIMT cells (virtual memory) dominate in aged mice. Cohousing with feral mice changed the bacterial colonization of laboratory strains but had only minimal effects on the CD8+ T cell compartment, including AIMT cells.
- 650 _2
- $a stárnutí $x genetika $x imunologie $7 D000375
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a antigeny $x genetika $x imunologie $7 D000941
- 650 _2
- $a klonální evoluce $7 D060965
- 650 _2
- $a nestabilita genomu $7 D042822
- 650 _2
- $a imunologická paměť $x genetika $x imunologie $7 D007156
- 650 _2
- $a myši $7 D051379
- 650 _2
- $a myši inbrední BALB C $7 D008807
- 650 _2
- $a myši inbrední C57BL $7 D008810
- 650 _2
- $a myši transgenní $7 D008822
- 650 _2
- $a fenotyp $7 D010641
- 650 _2
- $a T-lymfocyty $x imunologie $7 D013601
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Niederlova, Veronika $u Laboratory of Adaptive Immunity, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
- 700 1_
- $a Novotny, Jiri $u Laboratory of Genomics and Bioinformatics, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic $u Department of Informatics and Chemistry, Faculty of Chemical Technology, University of Chemistry and Technology, Prague, Czech Republic
- 700 1_
- $a Schmiedova, Lucie $u Department of Zoology, Faculty of Science, Charles University, Prague, Czech Republic
- 700 1_
- $a Kubovciak, Jan $u Laboratory of Genomics and Bioinformatics, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic $u Department of Zoology, Faculty of Science, Charles University, Prague, Czech Republic
- 700 1_
- $a Matejkova, Tereza $u Department of Zoology, Faculty of Science, BIOCEV, Charles University, Vestec, Czech Republic
- 700 1_
- $a Drobek, Ales $u Laboratory of Adaptive Immunity, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
- 700 1_
- $a Pribikova, Michaela $u Laboratory of Adaptive Immunity, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic $u Laboratory of Immunity & Cell Communication, First Faculty of Medicine, BIOCEV, Charles University, Vestec, Czech Republic
- 700 1_
- $a Stopkova, Romana $u Department of Zoology, Faculty of Science, BIOCEV, Charles University, Vestec, Czech Republic
- 700 1_
- $a Cizkova, Dagmar $u Research Facility Studenec, Institute of Vertebrate Biology, Czech Academy of Sciences, Brno, Czech Republic
- 700 1_
- $a Neuwirth, Ales $u Laboratory of Adaptive Immunity, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
- 700 1_
- $a Michalik, Juraj $u Laboratory of Adaptive Immunity, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
- 700 1_
- $a Krizova, Katerina $u Laboratory of Adaptive Immunity, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
- 700 1_
- $a Hudcovic, Tomáš, $u Laboratory of Gnotobiology, Institute of Microbiology of the Czech Academy of Sciences, Novy Hradek, Czech Republic; and $d 1965- $7 xx0267225
- 700 1_
- $a Kolar, Michal $u Laboratory of Genomics and Bioinformatics, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic $u Department of Informatics and Chemistry, Faculty of Chemical Technology, University of Chemistry and Technology, Prague, Czech Republic
- 700 1_
- $a Kozakova, Hana $u Laboratory of Gnotobiology, Institute of Microbiology of the Czech Academy of Sciences, Novy Hradek, Czech Republic; and
- 700 1_
- $a Kreisinger, Jakub $u Department of Zoology, Faculty of Science, Charles University, Prague, Czech Republic
- 700 1_
- $a Stopka, Pavel $u Department of Zoology, Faculty of Science, BIOCEV, Charles University, Vestec, Czech Republic
- 700 1_
- $a Stepanek, Ondrej $u Laboratory of Adaptive Immunity, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic; ondrej.stepanek@img.cas.cz $u Department of Biomedicine, University Hospital and University of Basel, Basel, Switzerland
- 773 0_
- $w MED00002741 $t Journal of immunology (Baltimore, Md. : 1950) $x 1550-6606 $g Roč. 206, č. 9 (2021), s. 2109-2121
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/33858960 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20211013 $b ABA008
- 991 __
- $a 20211208101103 $b ABA008
- 999 __
- $a ok $b bmc $g 1714719 $s 1146305
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2021 $b 206 $c 9 $d 2109-2121 $e 20210415 $i 1550-6606 $m The Journal of immunology $n J Immunol $x MED00002741
- GRA __
- $a 166538 $p Swiss National Science Foundation $2 Switzerland
- LZP __
- $a Pubmed-20211013
