• Je něco špatně v tomto záznamu ?

The colonisation of Czech travellers and expatriates living in the Czech Republic by colistin-resistant Enterobacteriaceae and whole genome characterisation of E. coli isolates harbouring the mcr-1 genes on a plasmid or chromosome: A cross-sectional study

M. Krutova, A. Kalova, E. Nycova, T. Gelbicova, R. Karpiskova, E. Smelikova, O. Nyc, P. Drevinek, J. Tkadlec

. 2021 ; 39 (-) : 101914. [pub] 20201113

Jazyk angličtina Země Nizozemsko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc21026316

BACKGROUND: Travellers were recognized as a risk cohort that can be colonized by mcr-1-mediated colistin-resistant Enterobacteriaceae. We aimed to investigate the carriage of mcr-mediated colistin resistance in Enterobacteriaceae in Czech travellers or expatriates residing temporarily in the Czech Republic. METHODS: Between August 2018 and September 2019, the stool samples were cultured in enrichment broth. The enriched cultures were tested for the presence of the mcr-1-8 genes and inoculated onto selective agar with colistin. Colistin-resistant Enterobacteriaceae were tested for the presence of the mcr-1-8 genes; the mcr-positive isolates were characterised by whole genome sequencing. RESULTS: From the 177 stool samples, 15 colistin-resistant Enterobacteriaceae isolates were cultured (7.9%); two of the E. coli isolates carried the mcr-1 gene (1.1%). In the E. coli multilocus sequence type (ST) 156, the mcr-1 gene was located in an ISApl1-mcr-1-orf-ISApl1 (Tn6330) and incorporated into the chromosome; in the E. coli ST23 isolate, the mcr-1 gene was harboured by the plasmid IncX4. Both of the mcr-1 positive E. coli isolates were multidrug-resistant and one isolate was an extended-spectrum β-lactamase producer (blaCTX-M-27). CONCLUSION: Patients with an international travel history should be monitored for the carriage of the mcr-1 gene in order to prevent its dissemination into healthcare settings.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21026316
003      
CZ-PrNML
005      
20211026133010.0
007      
ta
008      
211013s2021 ne f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.tmaid.2020.101914 $2 doi
035    __
$a (PubMed)33197647
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Krutova, Marcela $u Department of Medical Microbiology, Charles University, 2nd Faculty of Medicine and Motol University Hospital, Prague, Czech Republic. Electronic address: marcela.krutova@lfmotol.cuni.cz
245    14
$a The colonisation of Czech travellers and expatriates living in the Czech Republic by colistin-resistant Enterobacteriaceae and whole genome characterisation of E. coli isolates harbouring the mcr-1 genes on a plasmid or chromosome: A cross-sectional study / $c M. Krutova, A. Kalova, E. Nycova, T. Gelbicova, R. Karpiskova, E. Smelikova, O. Nyc, P. Drevinek, J. Tkadlec
520    9_
$a BACKGROUND: Travellers were recognized as a risk cohort that can be colonized by mcr-1-mediated colistin-resistant Enterobacteriaceae. We aimed to investigate the carriage of mcr-mediated colistin resistance in Enterobacteriaceae in Czech travellers or expatriates residing temporarily in the Czech Republic. METHODS: Between August 2018 and September 2019, the stool samples were cultured in enrichment broth. The enriched cultures were tested for the presence of the mcr-1-8 genes and inoculated onto selective agar with colistin. Colistin-resistant Enterobacteriaceae were tested for the presence of the mcr-1-8 genes; the mcr-positive isolates were characterised by whole genome sequencing. RESULTS: From the 177 stool samples, 15 colistin-resistant Enterobacteriaceae isolates were cultured (7.9%); two of the E. coli isolates carried the mcr-1 gene (1.1%). In the E. coli multilocus sequence type (ST) 156, the mcr-1 gene was located in an ISApl1-mcr-1-orf-ISApl1 (Tn6330) and incorporated into the chromosome; in the E. coli ST23 isolate, the mcr-1 gene was harboured by the plasmid IncX4. Both of the mcr-1 positive E. coli isolates were multidrug-resistant and one isolate was an extended-spectrum β-lactamase producer (blaCTX-M-27). CONCLUSION: Patients with an international travel history should be monitored for the carriage of the mcr-1 gene in order to prevent its dissemination into healthcare settings.
650    _2
$a antibakteriální látky $x farmakologie $7 D000900
650    _2
$a chromozomy $7 D002875
650    12
$a kolistin $x farmakologie $7 D003091
650    _2
$a průřezové studie $7 D003430
650    _2
$a bakteriální léková rezistence $x genetika $7 D024881
650    _2
$a Enterobacteriaceae $x genetika $7 D004755
650    _2
$a Escherichia coli $x genetika $7 D004926
650    12
$a proteiny z Escherichia coli $x genetika $7 D029968
650    _2
$a lidé $7 D006801
650    _2
$a mikrobiální testy citlivosti $7 D008826
650    _2
$a plazmidy $x genetika $7 D010957
651    _2
$a Česká republika $7 D018153
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Kalova, Alzbeta $u Department of Bacteriology, Veterinary Research Institute, Brno, Czech Republic; Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic
700    1_
$a Nycova, Elka $u Department of Clinical Microbiology, Bulovka Hospital, Czech Republic
700    1_
$a Gelbicova, Tereza $u Department of Bacteriology, Veterinary Research Institute, Brno, Czech Republic
700    1_
$a Karpiskova, Renata $u Department of Bacteriology, Veterinary Research Institute, Brno, Czech Republic
700    1_
$a Smelikova, Eva $u Department of Medical Microbiology, Charles University, 2nd Faculty of Medicine and Motol University Hospital, Prague, Czech Republic
700    1_
$a Nyc, Otakar $u Department of Medical Microbiology, Charles University, 2nd Faculty of Medicine and Motol University Hospital, Prague, Czech Republic
700    1_
$a Drevinek, Pavel $u Department of Medical Microbiology, Charles University, 2nd Faculty of Medicine and Motol University Hospital, Prague, Czech Republic
700    1_
$a Tkadlec, Jan $u Department of Medical Microbiology, Charles University, 2nd Faculty of Medicine and Motol University Hospital, Prague, Czech Republic
773    0_
$w MED00008669 $t Travel medicine and infectious disease $x 1873-0442 $g Roč. 39, č. - (2021), s. 101914
856    41
$u https://pubmed.ncbi.nlm.nih.gov/33197647 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20211013 $b ABA008
991    __
$a 20211026133016 $b ABA008
999    __
$a ok $b bmc $g 1715132 $s 1146823
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 39 $c - $d 101914 $e 20201113 $i 1873-0442 $m Travel medicine and infectious disease $n Travel Med Infect Dis $x MED00008669
LZP    __
$a Pubmed-20211013

Najít záznam

Citační ukazatele

Možnosti archivace