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iTRAQ®-based quantitative proteomics reveals the proteomic profiling of methicillin-resistant Staphylococcus aureus-derived extracellular vesicles after exposure to imipenem
J. Wang, J. Wang, Y. Wang, P. Sun, X. Zou, L. Ren, C. Zhang, E. Liu
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
Grantová podpora
81660352
National Natural Science Foundation of China
- MeSH
- extracelulární vezikuly * MeSH
- imipenem farmakologie MeSH
- methicilin rezistentní Staphylococcus aureus * genetika MeSH
- proteomika MeSH
- stanovení celkové genové exprese MeSH
- Publikační typ
- časopisecké články MeSH
This study sought to reveal the proteomic profiling of methicillin-resistant Staphylococcus aureus (MRSA)-derived extracellular vesicles (EVs) after exposure to imipenem. The advanced isobaric tags for relative and absolute quantitation (iTRAQ®) proteomic approach were used to analyze the alterations in MRSA-derived EV protein patterns upon exposure to imipenem. A total of 1260 EV proteins were identified and quantified. Among these, 861 differentially expressed exosome proteins (P < 0.05) were found. Multivariate analysis, Gene Ontology (GO) annotation, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were used to analyze the identified proteins. Enrichment analysis of GO annotations indicated that imipenem primarily regulated the metabolic processes in MRSA. The metabolism of differentially expressed proteins was found to be the most significant in the combined analysis of the KEGG pathway analysis. Based on the results from the STRING analysis, 50S ribosomal protein L16 (RplP) and 30S ribosomal protein S8 (RpsH) were involved in the imipenem-induced MRSA-derived EVs. These results provide vital information on MRSA-derived EVs, increasing our knowledge of the proteome level changes in EVs upon exposure to imipenem. Moreover, these results pave the way for developing novel MRSA treatments.
China CDC Key Laboratory for Medical Virology Ministry of Health Beijing 102206 China
Chinese National Influenza Center National Institute for Viral Disease Control and Prevention
Pediatrics Institute Children's Hospital Chongqing Medical University Chongqing 400014 China
Citace poskytuje Crossref.org
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- $a Wang, Jichun $u Department of Respiratory Medicine, Children's Hospital of Chongqing Medical University, No.136, Zhong Shan 2nd Road, Chongqing, 400014, Yuzhong District, China $u Department of Pediatrics, Affiliated hospital of Inner Mongolia Medical University, Hohhot, 010110, Inner Mongolia, China
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- $a This study sought to reveal the proteomic profiling of methicillin-resistant Staphylococcus aureus (MRSA)-derived extracellular vesicles (EVs) after exposure to imipenem. The advanced isobaric tags for relative and absolute quantitation (iTRAQ®) proteomic approach were used to analyze the alterations in MRSA-derived EV protein patterns upon exposure to imipenem. A total of 1260 EV proteins were identified and quantified. Among these, 861 differentially expressed exosome proteins (P < 0.05) were found. Multivariate analysis, Gene Ontology (GO) annotation, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were used to analyze the identified proteins. Enrichment analysis of GO annotations indicated that imipenem primarily regulated the metabolic processes in MRSA. The metabolism of differentially expressed proteins was found to be the most significant in the combined analysis of the KEGG pathway analysis. Based on the results from the STRING analysis, 50S ribosomal protein L16 (RplP) and 30S ribosomal protein S8 (RpsH) were involved in the imipenem-induced MRSA-derived EVs. These results provide vital information on MRSA-derived EVs, increasing our knowledge of the proteome level changes in EVs upon exposure to imipenem. Moreover, these results pave the way for developing novel MRSA treatments.
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