-
Je něco špatně v tomto záznamu ?
Restriction endonuclease T.Smu451I with new cleavage specificity-neoschizomer of T.AsuI
SR. Ra, MS. Kim, TR. Kim, GS. Yom, CI. Paek, KC. Ri
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
- MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- gelová chromatografie MeSH
- molekulová hmotnost MeSH
- restrikční endonukleasy typu II * chemie izolace a purifikace metabolismus MeSH
- Sphingobacterium * enzymologie MeSH
- substrátová specifita MeSH
- Publikační typ
- časopisecké články MeSH
A specific type II restriction endonuclease T.Smu451I has been purified to electrophoretic homogeneity from the frozen cells of soil bacterium Sphingobacterium multivorum 451 (formerly Flavobacterium multivorum 451), using ultrasonic grinding, nucleic acid removal by streptomycin sulfate, protein precipitation by ammonium sulfate and phosphocellulose P-11, DEAE-Cellulose DE-52, Hepharin-Sepharose CL-6B chromatography, and elucidated several characteristics of T.Smu451I. The molecular weight of the enzyme determined by gel filtration and SDS-polyacrylamide gel electrophoresis was calculated to be 45,000 ± 2000 D (dimer) and 23,000 ± 1000 D (monomer), respectively. The isoelectric point (pI) of T.Smu451I is 5.4. T.Smu451I recognizes pentanucleotide palindromic sequences 5'-GGNC↓C-3' and cleaves between C and C in position shown by arrow to produce 3'-cohesive terminus of trinucleotide. Therefore, T.Smu451I is a neoschizomer of T.AsuI.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc21028079
- 003
- CZ-PrNML
- 005
- 20211105134020.0
- 007
- ta
- 008
- 211105s2021 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/s12223-021-00874-0 $2 doi
- 035 __
- $a (PubMed)33950513
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Ra, Sung-Ryong $u Research Institute of Biotechnology, Life Science Faculty, Kim Il Sung University, Pyongyang, Democratic People's Republic of Korea. life1@ryongnamsan.edu.kp
- 245 10
- $a Restriction endonuclease T.Smu451I with new cleavage specificity-neoschizomer of T.AsuI / $c SR. Ra, MS. Kim, TR. Kim, GS. Yom, CI. Paek, KC. Ri
- 520 9_
- $a A specific type II restriction endonuclease T.Smu451I has been purified to electrophoretic homogeneity from the frozen cells of soil bacterium Sphingobacterium multivorum 451 (formerly Flavobacterium multivorum 451), using ultrasonic grinding, nucleic acid removal by streptomycin sulfate, protein precipitation by ammonium sulfate and phosphocellulose P-11, DEAE-Cellulose DE-52, Hepharin-Sepharose CL-6B chromatography, and elucidated several characteristics of T.Smu451I. The molecular weight of the enzyme determined by gel filtration and SDS-polyacrylamide gel electrophoresis was calculated to be 45,000 ± 2000 D (dimer) and 23,000 ± 1000 D (monomer), respectively. The isoelectric point (pI) of T.Smu451I is 5.4. T.Smu451I recognizes pentanucleotide palindromic sequences 5'-GGNC↓C-3' and cleaves between C and C in position shown by arrow to produce 3'-cohesive terminus of trinucleotide. Therefore, T.Smu451I is a neoschizomer of T.AsuI.
- 650 _2
- $a gelová chromatografie $7 D002850
- 650 12
- $a restrikční endonukleasy typu II $x chemie $x izolace a purifikace $x metabolismus $7 D015252
- 650 _2
- $a elektroforéza v polyakrylamidovém gelu $7 D004591
- 650 _2
- $a molekulová hmotnost $7 D008970
- 650 12
- $a Sphingobacterium $x enzymologie $7 D045249
- 650 _2
- $a substrátová specifita $7 D013379
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Kim, Myong-Suk $u Research Institute of Biotechnology, Life Science Faculty, Kim Il Sung University, Pyongyang, Democratic People's Republic of Korea
- 700 1_
- $a Kim, Tong-Ryul $u Research Institute of Biotechnology, Life Science Faculty, Kim Il Sung University, Pyongyang, Democratic People's Republic of Korea
- 700 1_
- $a Yom, Gi-Su $u Research Institute of Biotechnology, Life Science Faculty, Kim Il Sung University, Pyongyang, Democratic People's Republic of Korea
- 700 1_
- $a Paek, Chon-Il $u Research Institute of Biotechnology, Life Science Faculty, Kim Il Sung University, Pyongyang, Democratic People's Republic of Korea
- 700 1_
- $a Ri, Kum-Chol $u Korean Center for Culture Collection, Pyongyang, Democratic People's Republic of Korea
- 773 0_
- $w MED00011005 $t Folia microbiologica $x 1874-9356 $g Roč. 66, č. 4 (2021), s. 651-657
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/33950513 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20211105 $b ABA008
- 991 __
- $a 20211105134025 $b ABA008
- 999 __
- $a ok $b bmc $g 1719457 $s 1148624
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2021 $b 66 $c 4 $d 651-657 $e 20210505 $i 1874-9356 $m Folia microbiologica $n Folia microbiol. (Prague) $x MED00011005
- LZP __
- $a Pubmed-20211105
