-
Je něco špatně v tomto záznamu ?
In vitro assessment of the impact of nickel on the viability and steroidogenesis in the human adrenocortical carcinoma (NCI-H295R) cell line
N. Lukac, Z. Forgacs, H. Duranova, T. Jambor, J. Zemanova, P. Massanyi, B. Tombarkiewicz, S. Roychoudhury, Z. Knazicka
Jazyk angličtina Země Česko
Typ dokumentu časopisecké články
Free Medical Journals od 1998
PubMed Central od 2020
ProQuest Central od 2005-01-01
Medline Complete (EBSCOhost) od 2006-01-01
Nursing & Allied Health Database (ProQuest) od 2005-01-01
Health & Medicine (ProQuest) od 2005-01-01
ROAD: Directory of Open Access Scholarly Resources od 1998
Odkazy
PubMed
32901497
DOI
10.33549/physiolres.934452
Knihovny.cz E-zdroje
- MeSH
- adrenokortikální karcinom metabolismus patologie MeSH
- adrenokortikální nádory metabolismus patologie MeSH
- endokrinní disruptory farmakologie MeSH
- estradiol metabolismus MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- nikl farmakologie MeSH
- progesteron metabolismus MeSH
- techniky in vitro MeSH
- testosteron metabolismus MeSH
- viabilita buněk MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Nickel is a ubiquitous environmental pollutant, which has various effects on reproductive endocrinology. In this study, human adrenocortical carcinoma (NCI-H295R) cell line was used as an in vitro biological model to study the effect of nickel chloride (NiCl2) on the viability and steroidogenesis. The cells were exposed to different concentrations (3.90; 7.80; 15.60; 31.20; 62.50; 125; 250 and 500 microM) of NiCl2 and compared with control group (culture medium without NiCl2). The cell viability was measured by the metabolic activity assay. Production of sexual steroid hormones was quantified by enzyme linked immunosorbent assay. Following 48 h culture of the cells in the presence of NiCl2 a dose-dependent depletion of progesterone release was observed even at the lower concentrations. In fact, lower levels of progesterone were detected in groups with higher doses (>/=125 microM) of NiCl2 (P<0.01), which also elicited cytotoxic action. A more prominent decrease in testosterone production (P<0.01) was also noted in comparison to that of progesterone. On the other hand, the release of 17beta-estradiol was substantially increased at low concentrations (3.90 to 62.50 microM) of NiCl2. The cell viability remained relatively unaltered up to 125 microM (P>0.05) and slightly decreased from 250 microM of NiCl2 (P<0.05). Our results indicate endocrine disruptive effect of NiCl2 on the release of progesterone and testosterone in the NCI-H295R cell line. Although no detrimental effect of NiCl2 (=62.50 microM) could be found on 17beta-estradiol production, its toxicity may reflect at other points of the steroidogenic pathway.
AgroBioTech Research Centre Slovak University of Agriculture in Nitra Slovak Republic
Department of Life Science and Bioinformatics Assam University Silchar India
Literatura
- 000
- 00000naa a2200000 a 4500
- 001
- bmc21028128
- 003
- CZ-PrNML
- 005
- 20211129135923.0
- 007
- ta
- 008
- 211105s2020 xr ad f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.33549/physiolres.934452 $2 doi
- 035 __
- $a (PubMed)32901497
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xr
- 100 1_
- $a Lukáč, Norbert, $d 1969- $7 xx0248559 $u Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic, AgroBioTech Research Centre, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic
- 245 10
- $a In vitro assessment of the impact of nickel on the viability and steroidogenesis in the human adrenocortical carcinoma (NCI-H295R) cell line / $c N. Lukac, Z. Forgacs, H. Duranova, T. Jambor, J. Zemanova, P. Massanyi, B. Tombarkiewicz, S. Roychoudhury, Z. Knazicka
- 504 __
- $a Literatura
- 520 9_
- $a Nickel is a ubiquitous environmental pollutant, which has various effects on reproductive endocrinology. In this study, human adrenocortical carcinoma (NCI-H295R) cell line was used as an in vitro biological model to study the effect of nickel chloride (NiCl2) on the viability and steroidogenesis. The cells were exposed to different concentrations (3.90; 7.80; 15.60; 31.20; 62.50; 125; 250 and 500 microM) of NiCl2 and compared with control group (culture medium without NiCl2). The cell viability was measured by the metabolic activity assay. Production of sexual steroid hormones was quantified by enzyme linked immunosorbent assay. Following 48 h culture of the cells in the presence of NiCl2 a dose-dependent depletion of progesterone release was observed even at the lower concentrations. In fact, lower levels of progesterone were detected in groups with higher doses (>/=125 microM) of NiCl2 (P<0.01), which also elicited cytotoxic action. A more prominent decrease in testosterone production (P<0.01) was also noted in comparison to that of progesterone. On the other hand, the release of 17beta-estradiol was substantially increased at low concentrations (3.90 to 62.50 microM) of NiCl2. The cell viability remained relatively unaltered up to 125 microM (P>0.05) and slightly decreased from 250 microM of NiCl2 (P<0.05). Our results indicate endocrine disruptive effect of NiCl2 on the release of progesterone and testosterone in the NCI-H295R cell line. Although no detrimental effect of NiCl2 (</=62.50 microM) could be found on 17beta-estradiol production, its toxicity may reflect at other points of the steroidogenic pathway.
- 650 _2
- $a adrenokortikální nádory $x metabolismus $x patologie $7 D000306
- 650 _2
- $a adrenokortikální karcinom $x metabolismus $x patologie $7 D018268
- 650 _2
- $a nádorové buněčné linie $7 D045744
- 650 _2
- $a viabilita buněk $7 D002470
- 650 _2
- $a endokrinní disruptory $x farmakologie $7 D052244
- 650 _2
- $a estradiol $x metabolismus $7 D004958
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a techniky in vitro $7 D066298
- 650 _2
- $a nikl $x farmakologie $7 D009532
- 650 _2
- $a progesteron $x metabolismus $7 D011374
- 650 _2
- $a testosteron $x metabolismus $7 D013739
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Forgacs, Z. $u Independent Researcher, Budapest, Hungary
- 700 1_
- $a Ďúranová, Hana $7 xx0266733 $u AgroBioTech Research Centre, Slovak University of Agriculture in Nitra, Slovak Republic
- 700 1_
- $a Jambor, Tomáš $7 xx0248602 $u Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic
- 700 1_
- $a Zemanová, Jiřina $7 xx0267487 $u Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic
- 700 1_
- $a Massanyi, Peter, $d 1968- $7 uzp2012691017 $u Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic
- 700 1_
- $a Tombarkiewicz, B. $u Department of Zoology and Animal Welfare, Faculty of Animal Sciences, University of Agriculture in Krakow, Krakow, Poland
- 700 1_
- $a Roychoudhury, S. $u Department of Life Science and Bioinformatics, Assam University, Silchar, India
- 700 1_
- $a Kňažická, Zuzana $7 xx0266740 $u Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic
- 773 0_
- $w MED00003824 $t Physiological research $x 1802-9973 $g Roč. 69, č. 5 (2020), s. 871-883
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/32901497 $y Pubmed
- 910 __
- $a ABA008 $b A 4120 $c 266 $y p $z 0
- 990 __
- $a 20211105 $b ABA008
- 991 __
- $a 20211123103201 $b ABA008
- 999 __
- $a ok $b bmc $g 1728737 $s 1148673
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2020 $b 69 $c 5 $d 871-883 $e 20200909 $i 1802-9973 $m Physiological research $n Physiol. Res. (Print) $x MED00003824
- LZP __
- $b NLK118 $a Pubmed-20211105