INTRODUCTION: The inflammatory process in Crohn's disease (CD) is closely associated with the formation of reactive oxygen species. Antioxidant enzymes can play an important role in the outcome of CD and may influence postoperative recurrence in these patients. The aim of our study was to evaluate gene expression of intracellular antioxidant enzymes in surgically resected intestinal specimens of patients with CD, both in macroscopically normal and in inflamed tissue. METHODS: A total of 28 patients referred for elective bowel resection were enrolled in the study. Full-thickness small intestinal specimens were investigated. Gene expression of antioxidant enzymes - superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione reductase (GSR) - was evaluated both in macroscopically normal and inflamed samples. RESULTS: There were significantly lower levels of SOD1 mRNA (p = 0.007) and GSR mRNA (p = 0.027) in inflamed tissue compared to macroscopically normal areas. No significant differences were found between affected and non-affected intestinal segments in mRNA for SOD2, SOD3 and GPX. CONCLUSIONS: Our pilot data clearly showed that the gene expression of major antioxidant enzymes is not a uniform mechanism in the pathogenesis of Crohn's disease. Topically decreased gene expression of SOD1 and GSR might facilitate the segmental tissue injury caused by reactive oxygen species.

2nd Department of Internal Medicine Gastroenterology Charles University Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové Czech Republic

Department of Field Surgery Faculty of Military Health Sciences University of Defence Hradec Králové Czech Republic

Department of Medical Biochemistry Charles University Faculty of Medicine in Hradec Králové Czech Republic

Department of Surgery Charles University Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové Czech Republic

000      

00000naa a2200000 a 4500

001      

bmc21029383

003      

CZ-PrNML

005      

20211227134347.0

007      

ta

008      

211206s2021 xr d f 000 0|eng||

009      

AR

024    7_

$a 10.14712/18059694.2021.26 $2 doi

035    __

$a (PubMed)34779380

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a xr

100    1_

$a Sotona, Otakar $u Department of Field Surgery, Faculty of Military Health Sciences, University of Defence, Hradec Králové, Czech Republic. otakar.sotona@seznam.cz $u Department of Surgery, Charles University, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Czech Republic. otakar.sotona@seznam.cz $7 xx0140744

245    10

$a Gene Expression of Antioxidant Enzymes in the Resected Intestine in Crohn's Disease / $c O. Sotona, E. Peterová, J. Örhalmi, T. Dušek, A. Mrkvicová, V. Knoblochová, P. Lochman, O. Malý, J. Páral, J. Bureš

520    9_

$a INTRODUCTION: The inflammatory process in Crohn's disease (CD) is closely associated with the formation of reactive oxygen species. Antioxidant enzymes can play an important role in the outcome of CD and may influence postoperative recurrence in these patients. The aim of our study was to evaluate gene expression of intracellular antioxidant enzymes in surgically resected intestinal specimens of patients with CD, both in macroscopically normal and in inflamed tissue. METHODS: A total of 28 patients referred for elective bowel resection were enrolled in the study. Full-thickness small intestinal specimens were investigated. Gene expression of antioxidant enzymes - superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione reductase (GSR) - was evaluated both in macroscopically normal and inflamed samples. RESULTS: There were significantly lower levels of SOD1 mRNA (p = 0.007) and GSR mRNA (p = 0.027) in inflamed tissue compared to macroscopically normal areas. No significant differences were found between affected and non-affected intestinal segments in mRNA for SOD2, SOD3 and GPX. CONCLUSIONS: Our pilot data clearly showed that the gene expression of major antioxidant enzymes is not a uniform mechanism in the pathogenesis of Crohn's disease. Topically decreased gene expression of SOD1 and GSR might facilitate the segmental tissue injury caused by reactive oxygen species.

650    12

$a antioxidancia $7 D000975

650    12

$a Crohnova nemoc $x genetika $x metabolismus $7 D003424

650    12

$a exprese genu $7 D015870

650    _2

$a glutathionperoxidasa $x genetika $7 D005979

650    _2

$a lidé $7 D006801

650    _2

$a střeva $7 D007422

650    _2

$a messenger RNA $x genetika $7 D012333

650    _2

$a reaktivní formy kyslíku $7 D017382

650    _2

$a superoxiddismutasa $x genetika $7 D013482

650    12

$a superoxid dismutáza 1 $x genetika $x metabolismus $7 D000072105

655    _2

$a časopisecké články $7 D016428

700    1_

$a Peterová, Eva $u Department of Medical Biochemistry, Charles University, Faculty of Medicine in Hradec Králové, Czech Republic $7 xx0239580

700    1_

$a Örhalmi, Július $u Department of Surgery, Charles University, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Czech Republic $7 xx0087793

700    1_

$a Dušek, Tomáš, $u Department of Field Surgery, Faculty of Military Health Sciences, University of Defence, Hradec Králové, Czech Republic $u Department of Surgery, Charles University, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Czech Republic $d 1980- $7 xx0225241

700    1_

$a Mrkvicová, Alena $u Department of Medical Biochemistry, Charles University, Faculty of Medicine in Hradec Králové, Czech Republic $7 xx0267751

700    1_

$a Knoblochová, Veronika $u 2nd Department of Internal Medicine - Gastroenterology, Charles University, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Czech Republic $7 xx0252522

700    1_

$a Lochman, Petr $u Department of Field Surgery, Faculty of Military Health Sciences, University of Defence, Hradec Králové, Czech Republic $u Department of Surgery, Charles University, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Czech Republic $7 xx0092570

700    1_

$a Malý, Ondřej $u Department of Field Surgery, Faculty of Military Health Sciences, University of Defence, Hradec Králové, Czech Republic $u Department of Surgery, Charles University, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Czech Republic $7 xx0267750

700    1_

$a Páral, Jiří, $u Department of Field Surgery, Faculty of Military Health Sciences, University of Defence, Hradec Králové, Czech Republic $u Department of Surgery, Charles University, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Czech Republic $d 1963- $7 mzk2008469111

700    1_

$a Bureš, Jan, $u 2nd Department of Internal Medicine - Gastroenterology, Charles University, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Czech Republic $d 1954- $7 nlk19990073060

773    0_

$w MED00010947 $t Acta Medica (Hradec Kralove) $x 1805-9694 $g Roč. 64, č. 3 (2021), s. 153-157

856    41

$u https://pubmed.ncbi.nlm.nih.gov/34779380 $y Pubmed

910    __

$a ABA008 $b A 3077 $c 1072 $y p $z 0

990    __

$a 20211206 $b ABA008

991    __

$a 20211227134347 $b ABA008

999    __

$a ok $b bmc $g 1731383 $s 1149936

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2021 $b 64 $c 3 $d 153-157 $e 20211111 $i 1805-9694 $m Acta Medica $n Acta Med. (Hradec Král.) $x MED00010947

GRA    __

$a PROGRES Q40-15 $p Univerzita Karlova v Praze

GRA    __

$a PROGRES Q40-01 $p Univerzita Karlova v Praze

GRA    __

$a UHHK, 00179906 $p Ministerstvo Zdravotnictví Ceské Republiky

LZP    __

$b NLK138 $a Pubmed-20211206