Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

The Effects of Bilirubin and Lumirubin on the Differentiation of Human Pluripotent Cell-Derived Neural Stem Cells

N. Capková, V. Pospíšilová, V. Fedorová, J. Raška, K. Pospíšilová, M. Dal Ben, A. Dvořák, J. Viktorová, D. Bohačiaková, L. Vítek

. 2021 ; 10 (10) : . [pub] 20210927

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc22001677

Grantová podpora
RVO-VFN64165/2021 Ministerstvo Zdravotnictví Ceské Republiky
NV18-07-00342 Agentura Pro Zdravotnický Výzkum České Republiky
21-01799S Grantová Agentura České Republiky
18-25429Y Grantová Agentura České Republiky
INBIO (No. CZ.02.1.01/0.0/0.0/16_026/0008451) European Regional Development Fund
National Program of Sustainability II Project no. LQ1605 Ministerstvo Školství, Mládeže a Tělovýchovy

The 'gold standard' treatment of severe neonatal jaundice is phototherapy with blue-green light, which produces more polar photo-oxidation products that are easily excreted via the bile or urine. The aim of this study was to compare the effects of bilirubin (BR) and its major photo-oxidation product lumirubin (LR) on the proliferation, differentiation, morphology, and specific gene and protein expressions of self-renewing human pluripotent stem cell-derived neural stem cells (NSC). Neither BR nor LR in biologically relevant concentrations (12.5 and 25 µmol/L) affected cell proliferation or the cell cycle phases of NSC. Although none of these pigments affected terminal differentiation to neurons and astrocytes, when compared to LR, BR exerted a dose-dependent cytotoxicity on self-renewing NSC. In contrast, LR had a substantial effect on the morphology of the NSC, inducing them to form highly polar rosette-like structures associated with the redistribution of specific cellular proteins (β-catenin/N-cadherin) responsible for membrane polarity. This observation was accompanied by lower expressions of NSC-specific proteins (such as SOX1, NR2F2, or PAX6) together with the upregulation of phospho-ERK. Collectively, the data indicated that both BR and LR affect early human neurodevelopment in vitro, which may have clinical relevance in phototherapy-treated hyperbilirubinemic neonates.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22001677
003      
CZ-PrNML
005      
20220112153655.0
007      
ta
008      
220107s2021 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/antiox10101532 $2 doi
035    __
$a (PubMed)34679668
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Capková, Nikola $u Institute of Medical Biochemistry and Laboratory Diagnostics, Faculty General Hospital and 1st Faculty of Medicine, Charles University, 110 00 Prague, Czech Republic
245    14
$a The Effects of Bilirubin and Lumirubin on the Differentiation of Human Pluripotent Cell-Derived Neural Stem Cells / $c N. Capková, V. Pospíšilová, V. Fedorová, J. Raška, K. Pospíšilová, M. Dal Ben, A. Dvořák, J. Viktorová, D. Bohačiaková, L. Vítek
520    9_
$a The 'gold standard' treatment of severe neonatal jaundice is phototherapy with blue-green light, which produces more polar photo-oxidation products that are easily excreted via the bile or urine. The aim of this study was to compare the effects of bilirubin (BR) and its major photo-oxidation product lumirubin (LR) on the proliferation, differentiation, morphology, and specific gene and protein expressions of self-renewing human pluripotent stem cell-derived neural stem cells (NSC). Neither BR nor LR in biologically relevant concentrations (12.5 and 25 µmol/L) affected cell proliferation or the cell cycle phases of NSC. Although none of these pigments affected terminal differentiation to neurons and astrocytes, when compared to LR, BR exerted a dose-dependent cytotoxicity on self-renewing NSC. In contrast, LR had a substantial effect on the morphology of the NSC, inducing them to form highly polar rosette-like structures associated with the redistribution of specific cellular proteins (β-catenin/N-cadherin) responsible for membrane polarity. This observation was accompanied by lower expressions of NSC-specific proteins (such as SOX1, NR2F2, or PAX6) together with the upregulation of phospho-ERK. Collectively, the data indicated that both BR and LR affect early human neurodevelopment in vitro, which may have clinical relevance in phototherapy-treated hyperbilirubinemic neonates.
655    _2
$a časopisecké články $7 D016428
700    1_
$a Pospíšilová, Veronika $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University, 601 77 Brno, Czech Republic
700    1_
$a Fedorová, Veronika $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University, 601 77 Brno, Czech Republic
700    1_
$a Raška, Jan $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University, 601 77 Brno, Czech Republic
700    1_
$a Pospíšilová, Kateřina $u Institute of Medical Biochemistry and Laboratory Diagnostics, Faculty General Hospital and 1st Faculty of Medicine, Charles University, 110 00 Prague, Czech Republic
700    1_
$a Dal Ben, Matteo $u Institute of Medical Biochemistry and Laboratory Diagnostics, Faculty General Hospital and 1st Faculty of Medicine, Charles University, 110 00 Prague, Czech Republic
700    1_
$a Dvořák, Aleš $u Institute of Medical Biochemistry and Laboratory Diagnostics, Faculty General Hospital and 1st Faculty of Medicine, Charles University, 110 00 Prague, Czech Republic
700    1_
$a Viktorová, Jitka $u Department of Biochemistry and Microbiology, University of Chemistry and Technology, 166 28 Prague, Czech Republic
700    1_
$a Bohačiaková, Dáša $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University, 601 77 Brno, Czech Republic $u International Clinical Research Center (ICRC), St. Anne's University Hospital, 656 91 Brno, Czech Republic
700    1_
$a Vítek, Libor $u Institute of Medical Biochemistry and Laboratory Diagnostics, Faculty General Hospital and 1st Faculty of Medicine, Charles University, 110 00 Prague, Czech Republic $u 4th Department of Internal Medicine, Faculty General Hospital and 1st Faculty of Medicine, Charles University, 110 00 Prague, Czech Republic
773    0_
$w MED00200130 $t Antioxidants (Basel, Switzerland) $x 2076-3921 $g Roč. 10, č. 10 (2021)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/34679668 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20220107 $b ABA008
991    __
$a 20220112153651 $b ABA008
999    __
$a ind $b bmc $g 1745567 $s 1152824
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 10 $c 10 $e 20210927 $i 2076-3921 $m Antioxidants $n Antioxidants $x MED00200130
GRA    __
$a RVO-VFN64165/2021 $p Ministerstvo Zdravotnictví Ceské Republiky
GRA    __
$a NV18-07-00342 $p Agentura Pro Zdravotnický Výzkum České Republiky
GRA    __
$a 21-01799S $p Grantová Agentura České Republiky
GRA    __
$a 18-25429Y $p Grantová Agentura České Republiky
GRA    __
$a INBIO (No. CZ.02.1.01/0.0/0.0/16_026/0008451) $p European Regional Development Fund
GRA    __
$a National Program of Sustainability II Project no. LQ1605 $p Ministerstvo Školství, Mládeže a Tělovýchovy
LZP    __
$a Pubmed-20220107
Zpět

Najít záznam

Citační ukazatele

Možnosti archivace