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6S-Like scr3559 RNA Affects Development and Antibiotic Production in Streptomyces coelicolor

J. Bobek, A. Mikulová, D. Šetinová, M. Elliot, M. Čihák

. 2021 ; 9 (10) : . [pub] 20210922

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc22001713

Grantová podpora
UJEP-SGS-2020-53-002-2 Jan Evangelista Purkyně University in Ústí nad Labem
PROGRES Q25/LF1 Charles University
SVV260520 Charles University
04681 Natural Sciences and Engineering Research Council

Regulatory RNAs control a number of physiological processes in bacterial cells. Here we report on a 6S-like RNA transcript (scr3559) that affects both development and antibiotic production in Streptomyces coelicolor. Its expression is enhanced during the transition to stationary phase. Strains that over-expressed the scr3559 gene region exhibited a shortened exponential growth phase in comparison with a control strain; accelerated aerial mycelium formation and spore maturation; alongside an elevated production of actinorhodin and undecylprodigiosin. These observations were supported by LC-MS analyses of other produced metabolites, including: germicidins, desferrioxamines, and coelimycin. A subsequent microarray differential analysis revealed increased expression of genes associated with the described morphological and physiological changes. Structural and functional similarities between the scr3559 transcript and 6S RNA, and its possible employment in regulating secondary metabolite production are discussed.

Citace poskytuje Crossref.org

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