A complex evaluation of agonist bias at G-protein coupled receptors at the level of G-protein classes and isoforms including non-preferential ones is essential for advanced agonist screening and drug development. Molecular crosstalk in downstream signaling and a lack of sufficiently sensitive and selective methods to study direct coupling with G-protein of interest complicates this analysis. We performed binding and functional analysis of 11 structurally different agonists on prepared fusion proteins of individual subtypes of muscarinic receptors and non-canonical promiscuous α-subunit of G16 protein to study agonist bias. We have demonstrated that fusion of muscarinic receptors with Gα16 limits access of other competitive Gα subunits to the receptor, and thus enables us to study activation of Gα16 mediated pathway more specifically. Our data demonstrated agonist-specific activation of G16 pathway among individual subtypes of muscarinic receptors and revealed signaling bias of oxotremorine towards Gα16 pathway at the M2 receptor and at the same time impaired Gα16 signaling of iperoxo at M5 receptors. Our data have shown that fusion proteins of muscarinic receptors with α-subunit of G-proteins can serve as a suitable tool for studying agonist bias, especially at non-preferential pathways.

Department of Neurochemistry Institute of Physiology Czech Academy of Sciences 14220 Prague Czech Republic

Department of Physical Sciences Barry University Miami Shores FL 33161 USA

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$a Randáková, Alena $u Department of Neurochemistry, Institute of Physiology Czech Academy of Sciences, 14220 Prague, Czech Republic

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$a Nelic, Dominik $u Department of Neurochemistry, Institute of Physiology Czech Academy of Sciences, 14220 Prague, Czech Republic

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$a Hochmalová, Martina $u Department of Neurochemistry, Institute of Physiology Czech Academy of Sciences, 14220 Prague, Czech Republic

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$a Mulenga, Mutale Jane $u Department of Neurochemistry, Institute of Physiology Czech Academy of Sciences, 14220 Prague, Czech Republic

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$a Boulos, John $u Department of Physical Sciences, Barry University, Miami Shores, FL 33161, USA

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$a Jakubík, Jan $u Department of Neurochemistry, Institute of Physiology Czech Academy of Sciences, 14220 Prague, Czech Republic

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